MACHEREY-NAGEL – Products for thin layer chromatography (TLC/HPTLC)

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MACHEREY-NAGEL and Chromatography

We provide optimal and reliable solutions for your laboratory work in sample preparation, method development and routine analysis.

About Us

MACHEREY-NAGEL Chromatography – complete solutions for your analysis

From a pioneer in chromatography to a full-range supplier of laboratory consumables, we supply laboratories around the world with HPLC, GC and SPE columns, TLC/HPTLC plates, autosampler vials, closures and more.

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MACHEREY-NAGEL offers a versatile range of ready-to-use TLC and HPTLC layers, which are the result of 50 years of continuous research and development in thin layer chromatography.

The comprehensive range of TLC products from MACHEREY-NAGEL includes:


TLC/HPTLC glass plates

ADAMANT, SIL G or SIL HD are silica layers on glass backing. It’s hard to imagine a laboratory without our reliable TLC and HPTLC glass plates.


ALUGRAM and POLYGRAM TLC/HPTLC sheets are easy to handle, lightweight and flexible.

High performance silica on ALUGRAM Xtra sheets provides outstanding wettability for precise colourisation results. Moreover these TLC sheets are easy to cut with scissors.



In addition to high quality TLC plates and sheets, we offer staining reagents and developing chambers, as well as introductory sets for educational purposes.

TLC Backings & Supports
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TLC/HPTLC plates and sheets consist of an adsorbent stationary phase on a backing support.

MACHEREY-NAGEL offers three different backings:

  • Glass plates
  • Aluminium sheets
  • Polyester sheets

Properties of TLC backings

Glass Plates

  • Thickness (approx.): 1.3mm
  • Weight and storage requirements: high
  • Torsional strength: ideal
  • Temperature stability: high
  • Susceptible to breakage: yes
  • Can be cut with scissors: no
  • Chemical resistance against solvents: high
  • Chemical resistance against mineral acids and concentrated ammonia: high
  • Suitability for aqueous detection reagents: depends on phases (layer)

Glass plates are robust, heat proof and chemically resistant to all common mobile phases and visualisation reagents. They are the traditional option in TLC/HPTLC and are widely used in the pharmaceutical sector and other laboratory environments.

Aluminium Sheets - ALUGRAM Xtra

  • Thickness (approx.): 0.15mm
  • Weight and storage requirements: low
  • Torsional strength: relatively high
  • Temperature stability: high
  • Susceptible to breakage: no
  • Can be cut with scissors: yes
  • Chemical resistance against solvents: high
  • Chemical resistance against mineral acids and concentrated ammonia: low
  • Suitability for aqueous detection reagents: ALUGRAM: low to moderate, ALUGRAM Xtra: moderate to high

Aluminium sheets are easy to handle, lightweight and flexible. High performance silica on ALUGRAM Xtra sheets provides outstanding wettability for precise colourisation results, even with 100% aqueous detection reagents. Moreover, ALUGRAM Xtra sheets are easy to cut with scissors. No flaking of silica occurs!

Polyester Sheets – POLYGRAM

  • Thickness (approx.): 0.2mm
  • Weight and storage requirements: low
  • Torsional strength: low
  • Temperature stability: maximum 185°C
  • Susceptible to breakage: no
  • Can be cut with scissors: yes
  • Chemical resistance against solvents: high
  • Chemical resistance against mineral acids and concentrated ammonia: high
  • Suitability for aqueous detection reagents: good

Polyester sheets are easy to handle, lightweight and flexible, and can also be cut with scissors. Developed POLYGRAM sheets can also be stored for documentation in laboratory notebooks.


Features of modern TLC/HPTLC

The success of thin layer chromatography as a highly efficient microanalytical separation method is based on a large number of advantageous properties:

  • High sample throughput in a short time
  • Suitable for screening tests
  • Pilot procedure for HPLC and Flash chromatography
  • After separation the analytical information can be stored for a longer period of time (the TLC ready-to-use layer acts as storage medium for data)
  • Separated substances can be subjected to subsequent analytical procedures (e.g., IR, MS) at a later date
  • Rapid and cost-efficient optimisation of the separation due to easy change of mobile and stationary phase
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'TLC' and 'HPTLC' are both widely used terms in laboratory environments, but what is the difference? At MACHEREY-NAGEL, we define 'HPTLC' where at least one of the following criteria is being fulfilled:

  • Layers with particle size 2–10 μm
  • Layers prefixed with 'Nano'
  • Modified layers

Narrow fractionation of HPTLC silica particles allows:

  • Sharper separations
  • Shorter developing times
  • Shorter migration distances
  • Smaller samples needed
  • Increased detection sensitivity (compared to TLC)

Macherey-Nagel™ Chromafix™ PS-OH- cartridges

CHROMAFIX 200-PS-OH- Füllmenge: 200 mg, (VE=50Stck.)

Macherey-Nagel™ pH-Fix Test Strips in PT Tubes

Provides quick and easy pH value testing at the point of interest. Macherey Nagel™ pH-Fix Test Strips in PT Tubes don't need any calibration, set-up or maintenance.
pH-Fix Indikatorstäbchen pH 0 - 14 Dosen à 100Teststäbchen

Macherey-Nagel™ Chromabond™ Leur Fitting

X12 CHROMABOND Luer fitting with lid male for Vacuum manifold

Macherey-Nagel™ Type N11 Clear With Label and Scale Snap Cap Vials

Schnappringgläser N 11-1, farblos mit Be-schriftungsfeld,breite Öffnung(VE=100St.)

Macherey-Nagel™ Nanaocolor™ Standard Test

Detect the least amounts of detectable substances. Macherey-Nagel™ Nanocolor™ Standard Tests are convenient reagent kits that use rectangular cuvettes with a 50mm optical path for photometric analysis. The use of a large sample volume guarantees safe and reproducible results. NANOCOLOR Mangan Rechteckküvettentest (ca. 250Bestimmungen)

Macherey-Nagel™ Glass Laboratory Sprayer

Used as an accessory in thin layer chromatography applications. Macherey-Nagel™ Glass Laboratory Sprayer includes a 100mL, clear glass conical flask with a sprayer-stopper assembly and rubber bulb/tubing. Glass from laboratory sprayer with rubber bulb

Macherey-Nagel™ Chromafix™ PS-Ag+ cartridges

CHROMAFIX Clean-up Kartuschen für Ionen-chromatographie 400-PS-AG, (VE=50St)

Macherey-Nagel™ pH Paper

Measure pH simply and easily. Macherey-Nagel™ pH paper comes in a circular format with a wide variety of pH ranges. 5M PH PAPER 4,0-7,0(VE=1 Rolle a 5m)

Macherey-Nagel™ TLC Simultaneous Developing Chamber

Develop multiple TLC plates simultaneously. TLC Simultaneous Developing Chambers  come with a choice of room for either 2 or 5 plates. TLC-Separation-Chamber for 2 simultaneous separations, 10 x 10 cm plates

Macherey-Nagel™ Crimp Neck Vials, N20, Clear

Store material for use in chromatography. Macherey-Nagel Crimp Neck Vials, N20, Clear are hydrolytic glass vials with 20mm crimp neck closures. Vials are manufactured under strict hygienic conditions to ensure high quality for a safe analysis. Available in a range of shapes and sizes. Probengläser N 20-10 HS m. abgerundetemBoden/konischem Bördelrand (VE=100Stck.)

Macherey-Nagel™ Folded MN 514 Filter Paper

x100 filter papers folded MN 5141/4 32 cm diameter, 100 pcs.

Macherey-Nagel™ N9 Polypropylene Blue Screw Caps

Seal vials with screw caps with a secure septum to receive needles. The Macherey-NagelTM N9 pollypropylene blue screw caps come with a choice of septa. X100 N 9 PP screw cap, blue, center hole,Silicone white/PTFE red, Hardness 45° shore A

Macherey-Nagel™ Chromafil™ Multi 96 Filter Plates

Chromab. Multi 96,RC-Filter,Monob,0,45µm

Macherey-Nagel™ Insert for Small Opening Vials, With Plastic Spring

Use with N8 Screw Neck, N11 Crimp Neck or other small neck vials. Macherey-Nagel™ Insert for Small Opening Vials, With Plastic Spring are 0.1mL glass inserts with a plastic spring that acts as a shock absorber for analyzing micro-samples using an autosampler. Mikroeinsatz 0,25ml für N8-1 Probengläser mont.PP-Feder, 5 x 29mm, VE=100 Stück

Macherey-Nagel™ Chromafix™ PS-Ag+ SPE Cartridge

CHROMAFIX 200-PS-Ag+ Füllmenge: 200 mg, (VE=50Stck.)

Macherey Nagel Nanocolor Tube Test

Fast, easy-to-use, bar-coded, predosed test tubes for testing a variety of water and wastewater analysis parameters. NANO BARCODE TEST NONIONI C SURFACTANTS

Macherey-Nagel™ Optima™ 1 Capillary Column for GC

Capillary column OPTIMA-1 ID: 0.25 mm, film thickness: 0.25 µm length: 25 mm,

Macherey-Nagel™ Screw Neck Vial, N9, Clear

Store liquid samples for use in chromatography. Macherey-Nagel™ Screw Neck Vial, N9, Clear are hydrolytic glass vials with wide openings and screw cap closures with a 9mm short screw thread. Vials are manufactured under strict hygienic conditions to ensure high quality for a safe analysis. Probgl N 9-1, farblos, Schraubge. / 100 (VE= 100PCE )

Macherey-Nagel™ MN 616 Grade Filter Papers, Folded

Particularly suited for general laboratory filtrations. Macherey-Nagel™ MN 616 Grade Filter Papers, Folded is a smooth qualitative paper with medium fast filtration speed. The average ash content is about 0.1%, and the amount of α-cellulose is about 95%. X100 FOLDED FILTER MN616 185MM

Macherey-Nagel™ Glass N11-1/N8-1 Small Opening Micro Insert

Mikroeinsätze für Probengläser N11-1/N8-1 farblos, stark verjüngt,0,2ml, (VE=100St)

Macherey-Nagel™ pH Indicator Paper Refill

Provides optimal quality and reliable readings. Macherey Nagel™ pH Indicator Paper Refills are supplied in plastic reels that ensure long-term stability and protection against many external influences. X3 REFILL 5M 1-14 PH

Macherey-Nagel™ Chromafil™ Polyvinylidene Difluoride Syringe Filters

For aqueous samples, water-soluble oligomers and polymers like proteins.   Macherey Nagel™ Chromafil™ Polyvinylidene Difluoride Syringe Filters are labeled for method validation and certification. X400 CHROMAFIL Xtra PVDF Einmalfilter 0,45µm,25mmBigBox VE=400 Stück

Macherey-Nagel™ Quantofix™ Peroxide 25 Semi-quantitative Test Strips

Ideal to test low levels of peroxide in solutions.  Macherey Nagel™ Quantofix™ Peroxide 25 Semi-quantitative Test Strips use an easy dip-and-read procedure and provide reliable results within 15 seconds. X100 Test sticks QUANTOFIX Peroxide 25 semi,

Macherey-Nagel™ Chromafil™ Xtra PES Polyethersulfone Syringe Filters

Labeled for method validation. Macherey-Nagel™ Chromafil™ Xtra PES Syringe Filters are hydrophilic membranes with very low adsorption and good stability with acids and bases. It is ideal for aqueous and slightly organic liquids, especially with pharmaceuticals and proteins. CHROMAFIL Xtra disposable filter PES-500/25membrane material: polyether sulfone pore dia.:

Macherey-Nagel™ Chromofil™ Glass Fiber Syringe Filter

Chromafil Einmalfilter zur KlarfiltrationGF-100/15 MS,Poren D=1,0µm, Membran D=15mm

Macherey-Nagel™ Visocolor™ ECO Test Kit Refill Pack

Replace consumed chemicals from test kits. Macherey-Nagel™ Visocolor™ ECO Test Kit Refill Packs are business-priced reagent sets used with ECO test kits. The kits use precisely-dosed single reagents, and results are evaluated visually with high-quality color comparison cards. REFILL CHROMIUM VINachfüllpackung (ca. 140 Bestimmungen)

Macherey-Nagel™ Chromafil™ Xtra PTFE Syringe Filters

Filter nonpolar liquids and gases. Chromafil™ Xtra PTFE Syringe Filters with polytetrafluoroethylene hydrophobic membrane. X400 Chromafil Xtra PTFE-45/25, BigBox

Macherey-Nagel™ Nucleodur™ 100-3 C18 ec HPLC Columns

EC HPLC column EC 50/3 NUCLEODUR 100-3 C18 ec length: 50 mm, ID: 3 mm

Macherey-Nagel™ Nucleosil™ 100-5 C8 HPLC Column

Use nonpolar phases for RP chromatography. Nucleosil™ 100-5 C8 HPLC Columns are not endcapped and come in a variety of diameters and lengths. EC HPLC column EC 125/4.6 NUCLEOSIL 100-5 C8 length: 125 mm, ID: 4.6 mm