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Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up, kit for gel extraction or PCR clean up
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Antal reaktioner:
240 forberedelser
50 forberedelser
Pakningsstørrelse:
240 forberedelser
50 reaktioner
Beskrivelse
NucleoSpin™ Gel and PCR Clean-up is designed for fast purification of PCR products, such as DNA from enzymatic reactions, as well as the extraction of DNA fragments from TAE or TBE agarose gels.
- Two applications in one kit—One buffer to process both sample types, with only one loading step
- High recovery for fragments down to 50 bp—Average recovery from 60–90%
- Minimized elution volume of 15μL—Highly concentrated DNA
- Binding buffer with pH indicator—Ensures optimal binding conditions
- Separate buffers for single stranded DNA and RNA or for SDS-containing samples available separately
- Suitable for all kinds of agarose gels and a variety of buffer systems (e.g. TAE, TBE)
- Complete removal of contaminations: nucleotides, primers, enzymes, mineral oil, PCR additives, detergents, dyes
- Applications: purification of PCR products, extraction of DNA from agarose or polyacrylamide gels, and downstream applications (cloning, sequencing, PCR, restriction analysis)
Kit contents: NucleoSpin™ Gel and PCR Clean-up columns, collection tubes (2mL), binding buffer with pH indicator, wash buffer and elution buffer
Tekniske data
Tekniske data
| Farve | pH Indicator: Yellow = correct conditions Green = pH slightly high (adjustment recommended) Blue = pH too high (adjustment necessary) |
| Format | Mini spinsøjle |
| Isoleringsteknologi | Silica membranteknologi |
| Effektivitet | Typical recovery: 70–95% |
| Elueringsvolumen | Elution volume: 30–100μL |
| Til brug med (udstyr) | QIAcube™ compatible TAE or TBE agarose gels, PCR |
| Inkluderer | Binding Buffer NTI (2 x 75mL), Wash Buffer NT3 Concentrate (60mL), Elution Buffer NE (125mL), NucleoSpin Gel and PCR Clean-up Columns (4 x 60) |
| Antal reaktioner | 240 forberedelser |
| Mængde | 1 sæt |
| Renhed | A260/A280: 1.8–1.9 |
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Sikkerhed og håndtering
Produktidentifikator
- NucleoSpin Gel and PCR Clean-up
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