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Cytiva HiTrap™ IEX HP Prepacked Columns
Use for high-resolution, small-scale protein purification. Cytiva Life Sciences™ HiTrap™ IEX HP Prepacked Columns are prepacked, ready-to-use Sepharose™ High Performance strong cation exchange columns.
Cytiva HiTrap™ Protein A HP Prepacked Columns
Use for purification and isolation of monoclonal and polyclonal IgG from ascites, serum and cell culture supernatants. Cytiva Life Sciences™ HiTrap™ Protein A HP Prepacked Columns are specially designed, together with the medium to provide fast, simple and easy separations in a convenient format.
Content And Storage | 4°C to 8°C, 20% Ethanol |
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For Use With (Equipment) | For syringe, peristaltic pump or a chromatography system |
Binding Capacity | 20 mg/mL IgG Medium |
For Use With (Application) | Chromatography System, Manual |
Cytiva HiTrap™ Q Sepharose Fast Flow IEX Columns
Use for small-scale protein purifications. Cytiva Life Sciences™ HiTrap™ Q Sepharose Fast Flow IEX Columns are strong anion exchangers for rapid separations either alone or connected in series.
Content And Storage | 4°C to 30°C in 20% Ethanol |
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Description | HiTrap Q FF |
Particle Size | 75μm |
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Buffer | Aqueous Buffer |
Ligand Type | N-benzyl-n-methyl Ethanolamine |
Height (Metric) | 25mm |
Content And Storage | 4° to 30°C |
pH Range | 3 to 12 |
Product Type | HiTrap Capto adhere column |
Column Type | Affinity Column |
Material | Polypropylene |
For Use With (Application) | Easy screening and convenient process development |
Support Type | Highly Cross-linked Agarose |
Height (English) | 0.98 in. |
Cytiva HiTrap™ Heparin HP Columns
Purify DNA-binding proteins, coagulation factors, lipoproteins and protein synthesis factors. Cytiva Life Sciences™ HiTrap™ Heparin HP Columns are prepacked with Heparin Sepharose™ High Performance for high-resolution purification.
Particle Size | 34 μm |
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Ligand Type | 10mg Heparin/mL medium |
Height (Metric) | 2.5cm |
Content And Storage | 4° to 30°C |
pH Range | 5 to 10 |
Product Type | Heparin HP Column |
For Use With (Equipment) | Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system |
Sample Type | DNA-binding Proteins, coagulation facto rs, Lipoproteins and Protein synthesis facto rs |
Material | Polypropylene |
For Use With (Application) | Purification of Antithrombin III, coagulation facto rs and other Plasma Proteins |
Support Type | 6% Highly Cross-linked Agarose |
Max. Pressure | 42psi |
Height (English) | 0.98 in. |
Cytiva HiTrap IXSelect
HiTrap IXSelect columns are prepacked with IXSelect resin for screening and small-scale protein purifications using affinity chromatography.
Content And Storage | 2°C to 8°C |
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Cytiva HiTrap™ Desalting Prepacked Columns
For rapid convenient desalting and buffer exchange. HiTrap™ Desalting Prepacked Columns are prepacked, ready to use column for group separation between high and low molecular weight substances.
Disposable | No |
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Particle Size | >20 μm (dry), 15 to 88 μm (wet) |
Content And Storage | 4°C to 30°C in 20% Ethanol |
Diameter (English) Inner Column | 0.62 in. |
Description | HiTrap Desalting are ready-to-use 5 mL columns prepacked with Sephadex G-25 Superfine resin for fast and convenient desalting and buffer exchange. |
Volume (Metric) Bed | 5 mL |
For Use With (Application) | For rapid convenient desalting and buffer exchange |
Diameter (Metric) Inner Column | 16 mm |
Cytiva HiTrap™ GCSFSelect
Prepacked columns specifically designed for the purification of recombinant Granulocyte-colony stimulating factor (G-CSF). Based on a highly rigid agarose base matrix that allows for high flow rates at large production scales.
Particle Size | 75 μm |
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Ligand Type | Single-Chain Antibody Fragment Directed Against G-CSF |
Type | Column |
For Use With | Purification of Recombinant Granulocyte-Colony Stimulating Factor (G-CSF) |
pH | 2 to 12 |
Quantity | 5/Pk. |
Purification Target | Granulocyte-Colony Stimulating Factor (G-CSF) |
Cytiva HiTrap Capto Butyl
Capto Phenyl (High sub) and Capto Butyl are hydrophobic interaction chromatography (HIC) resins used in the capture and intermediate stages of protein purification
Height (Metric) | 25 mm |
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Flow Rate | < 600 cm/h |
Storage Requirements | 4 to 30 °C, 20% Ethanol |
For Use With (Application) | Hydrophobic Interaction Chromatography |
Cytiva HiTrap™ Butyl HP Columns
Prepacked columns for convenient and fast separations. Cytiva HiTrap™ Butyl HP Columns are used for small-scale protein purification as well as for screening of binding and elution conditions.
Particle Size | 24 to 44 μm |
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Ligand Type | Butyl |
Height (Metric) | 30 mm |
pH Range | 3 to 13 |
Product Type | HiTrap Butyl HP |
Material | Polypropylene |
Storage Requirements | 4°C to 30°C, 20% Ethanol |
For Use With (Application) | For Screening of Selectivity, Binding and Elution Conditions, as Well as Small Scale Purifications |
Support Type | Cross-linked Agarose |
Media Type | Butyl Sepharose |
Max. Pressure | 5 bar [0.5 MPa] (70 psi) |
Height (English) | 1.181 in. |
Cytiva HiTrap Capto MMC Columns
HiTrap Capto MMC is prepacked with Capto MMC, a multimodal BioProcess ion exchange medium. The multimodal functionality gives a different selectivity compared with traditional ion exchangers and it binds proteins at high or low ionic strength.
Particle Size | 75μm |
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Buffer | Aqueous Buffer |
Height (Metric) | 25mm |
Content And Storage | 4° to 30°C |
pH Range | 2 to 12 |
Product Type | HiTrap Capto MMC column |
Column Type | Affinity Column |
Material | Polypropylene |
For Use With (Application) | Bind Proteins at the conductivity of the feed material and to solve specific purification challenges |
Support Type | Highly Cross-linked Agarose |
Height (English) | 0.98 in. |
Cytiva HiTrap™ Streptavidin HP Column
Use for fast and easy preparative purifications either alone or connected in series. HiTrap™ Streptavidin HP Column are convenient and reliable prepacked columns.
Cytiva HiTrap™ NHS-Activated HP Columns
Couple ligands containing primary amines easily. Cytiva Life Sciences™ HiTrap™ NHS-Activated HP Columns are prepacked with NHS activated Sepharose™ High Performance.
Particle Size | 34 μm |
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Ligand Type | 10μM NHS groups/mL medium |
Height (Metric) | 2.5cm |
Content And Storage | 4° to 8°C |
pH Range | 3 to 12 |
Product Type | NHS-Activated HP Column |
For Use With (Equipment) | Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system |
Material | Polypropylene |
For Use With (Application) | Preparative affinity chromato graphy, easy coupling of ligands containing primary Amines |
Support Type | 6% Highly Cross-linked Agarose |
Max. Pressure | 42psi |
Height (English) | 0.98 in. |
Cytiva HiTrap™ Phenyl HP Columns
Prepacked columns for convenient and fast separations. Cytiva Lifescience™ HiTrap™ Phenyl HP Columns are designed for use with a syringe, laboratory pump or chromatography system.
Particle Size | 24 to 44 μm |
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Ligand Type | Phenyl |
pH Range | 3 to 13 |
Product Type | HiTrap Phenyl HP |
Material | Polypropylene |
Storage Requirements | 4°C to 30°C, 20% Ethanol |
For Use With (Application) | For Screening of Selectivity, Binding and Elution Conditions, as Well as Small Scale Purifications |
Support Type | 6% Cross-linked Agarose |
Media Type | Phenyl Sepharose HP |
Max. Pressure | 5 bar [0.5 MPa] (70 psi) |