Cell Function Reagents and Kits
Cell Function Reagents and Kits
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Invitrogen™ LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells
Useful with a variety of fluorescence-detection methodologies
Invitrogen™ alamarBlue™ and alamarBlue™ HS Cell Viability Reagents
The alamarBlue cell viability reagents are ready-to-use resazurin-based solutions that function as cell health indicators by using the reducing power of living cells to quantitatively measure viability.
| Shipping Condition | Wet Ice |
|---|---|
| Content And Storage | Store in refrigerator at 2°C to 8°C Protect from light. |
| Form | Liquid |
| Product Type | Cell Viability Reagent |
| Emission | 590 |
| Concentration | 10X |
| For Use With (Application) | Viability Assay |
| Product Line | alamarBlue |
| Detection Method | Colorimetric, Fluorescence |
Invitrogen™ CellTrace™ Violet Cell Proliferation Kit, for flow cytometry
CellTrace™ Violet Cell Proliferation Kit, for flow cytometry
| Shipping Condition | Room Temperature |
|---|---|
| Format | Tube(s) |
| Product Type | Cell Proliferation Kit |
| For Use With (Equipment) | Flow Cytometer |
| Solubility | DMSO (Dimethylsulfoxide) |
| Emission | 405/450 |
| For Use With (Application) | Proliferation Assay |
| Product Line | CellTrace |
| Detection Method | Fluorescence |
Invitrogen™ Amplex™ Red Hydrogen Peroxide/Peroxidase Assay Kit
Sensitive, one-step assay to detect hydrogen peroxide (H2O2) or peroxidase activity
Invitrogen™ Image-iT™ TMRM Reagent (mitochondrial membrane potential indicator)
Healthy mitochondrial membranes maintain a difference in electrical potential between the interior and exterior of the organelle, referred to as a membrane potential.
Invitrogen™ Image-iT™ Cell Painting Kit
The Image-iT Cell Painting Kit conveniently provides six different fluorescent labels to assess cellular morphology by high content screening for drug discovery.
| Format | Kit |
|---|---|
| Sub Cellular Localization | Cytoskeleton,Plasma Membrane,Golgi,Mitochondria,Cytoplasm,Nucleus,Endoplasmic Reticulum,Nucleolus |
| Product Type | Cell Painting Kit |
| For Use With (Equipment) | Fluorescence Microscope,High Content Analysis Instrument,CellInsight CX7 Pro,CellInsight CX7 LZR Pro |
| Color | Multicolor |
| Label Type | Fluorescent Dye |
| For Use With (Application) | High-content Screening (HCS),Morphological Cell Profiling,Multiparameter Screening,Fluorescent Labeling,3D Labeling |
| Product Line | Image-iT |
| Shipping Condition | Room Temperature |
|---|---|
| Modification | Chemical |
| Sub Cellular Localization | Nucleus, Organelles, Cytoplasm |
| Form | Powder |
| For Use With (Equipment) | Confocal Microscope,Fluorescence Microscope,High Content Analysis Instrument,HTS Reader,Microplate Reader,Fluorescent Imager |
| Color | Green |
| Emission | Visible |
| Dye Type | Fluorescent Dye-Based |
| Product Line | Molecular Probes |
| Detection Method | Fluorescence |
Invitrogen™ CyQUANT™ LDH and G6PD Cytotoxicity Assays
CyQUANT cytotoxicity assays measure cellular toxicity based on the release of cytosolic enzymes (LDH or G6PD) from damaged cells. These cytotoxicity assays utilize either colorimetric or fluorescence detection and allow continuous monitoring of cell death using microplate readers.
| Cell Permeability | Cell-impermeant |
|---|---|
| Format | 96-well plate |
| Product Type | LDH Cytotoxicity Assay |
| For Use With (Equipment) | Microplate Reader |
| Color | Red |
| Dye Type | Other Label(s) or Dye(s), Resorufin |
| Product Line | CyQUANT™ |
| Detection Method | Colorimetric, Fluorescence |
Molecular Probes™ Fluo-4, AM, cell permeant
Fluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels.
Invitrogen™ Calcein AM, cell-permeant green and blue dyes
Calcein AM is a cell-permeant dye that can be used to determine cell viability in most eukaryotic cells. In live cells, the non-fluorescent Calcein AM is converted to green or blue fluorescent Calcein, after acetoxymethyl ester hydrolysis by intracellular esterases.
| Shipping Condition | Room Temperature |
|---|---|
| Content And Storage | Store in freezer (-5°C to -30°C) and protect from light. |
| For Use With (Equipment) | Fluorescence Microscope |
| Reagent Type | Cell Tracker Compounds, Cell Labeling Reagents |
| Dye Type | Other Label(s) or Dye(s) |
| For Use With (Application) | Cell Tracing,Cell Tracker |
Invitrogen™ LIVE/DEAD™ Cell Imaging Kit (488/570)
Sensitive two-color fluorescence cell viability assay optimized for FITC and Texas Red™ filters
Molecular Probes™ CyQUANT™ Cell Proliferation Assay, for cells in culture
CyQUANT™ Cell Proliferation Assay Kit, for cells in culture
Molecular Probes™ LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit, for UV excitation
The LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining, or prior to elimination of biohazardous materials using formaldehyde fixation.
| Shipping Condition | Room Temperature |
|---|---|
| dimExcitationWavelengthRange | 350 nm |
| Description | LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit, for UV excitation |
| Form | Solid |
| Solubility | DMSO (Dimethylsulfoxide) |
| Color | Blue |
| Emission | 450 |
| Dye Type | LIVE/DEAD™ Fixable Blue Dead Cell Stain |
| For Use With (Application) | Viability Assay |
| Detection Method | Fluorescence |
| Cell Type | Eukaryotic Cells |
| Cell Permeability | Impermeant |
| Format | Tube(s) |
| Product Type | Dead Cell Stain Kit |
| For Use With (Equipment) | Flow Cytometer |
| Product Line | LIVE/DEAD |
Invitrogen™ CellTrace™ CFSE Cell Proliferation Kit, for flow cytometry
CellTrace™ CFSE Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry.
| Shipping Condition | Room Temperature |
|---|---|
| Format | Tube(s) |
| Product Type | Cell Proliferation Kit |
| For Use With (Equipment) | Flow Cytometer |
| Emission | 488 |
| Product Line | CellTrace |
| Detection Method | Fluorescence |
Invitrogen™ Amplex™ Red and Amplex™ UltraRed Reagents
The Amplex Red and UltraRed reagents are fluorogenic substrates for peroxidase. These highly sensitive and stable probes react with H2O2 in the presence of HRP for solution-based assays and ELISAs. Amplex UltraRed is a brighter version of Amplex Red with an improved pH range and sensitivity.
| Substrate | HRP (Horseradish Peroxidase) Substrate |
|---|---|
| Shipping Condition | Room Temperature |
| Content And Storage | Store in freezer (-5°C to -30°C). |
| Form | Solid |
| Product Type | Red Hydrogen Peroxide |
| For Use With (Equipment) | Fluorometer,Microplate Reader |
| Validated Application | Cell Analysis |
| For Use With (Application) | Hydrogen Peroxidase Assay |
| Product Line | Amplex |
| Detection Method | Fluorescence |