Western Blotting Reagents, Membranes, Supplies

Merck Millipore Immobilon™-FL PVDF Transfer Membranes

Designed specifically for Western blots and protein sequencing. Immobilon™-FL PVDF Transfer Membranes are plain surfaced with 0.45μm pores and have lower background fluorescence than other membranes. Available in 10cm and 20cm squares and a 3.75m roll.

Cytiva Amersham™ Hyperfilm™ ECL

Use with all blue and green light chemiluminescent systems. Cytiva Amersham™ Hyperfilm™ ECL is constructed from a clear base coated on both sides with a photographic emulsion that is protected by an anti-scratch layer.

Merck Millipore Immobilon™ Western Chemiluminescent HRP Substrate (ECL)

Provides high sensitivity over a broad detection range at an economical price

Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

Thermo Scientific™ Pierce™ CN/DAB Substrate Kit

Combines chloronaphthol- and diaminobenzidine-based HRP detection for Western blot and tissue staining methods.

Invitrogen™ Mini Blot Module

Wet transfer device used exclusively with the Bolt Minigel Tank

Thermo Scientific™ Lab Vision™ UltraVision™ ONE Detection System: HRP Polymer (Ready-To-Use)

Take advantage of a one-step polymer system providing increased sensitivity, time savings and detection simplicity with the Thermo Scientific™ Lab Vision™ UltraVision™ ONE Detection System: HRP Polymer (Ready-To-Use).

Thermo Scientific™ 1-Step™ TMB-Blotting Substrate Solution

Ready-to-use blue TMB peroxidase (HRP) substrate specially formulated for chromogenic detection in Western blot and IHC experiments.

Invitrogen™ iBlot™ 2 Transfer Stacks, nitrocellulose, regular size

Used to transfer proteins with the iBlot 2 Gel Transfer Device

Cytiva Whatman™ Grade GB003 Blotting Paper

Ensures that no contamination will occur during the transfer steps. Whatman™ Grade GB003 Blotting Paper eliminates sheet to sheet variations with precisely cut membrane sizes.

Invitrogen™ iBlot™ 2 Gel Transfer Device

Performs Western blotting transfer simply, efficiently, and reliably, within seven minutes and without the need for liquid buffers

Invitrogen™ Shrimp Alkaline Phosphatase (SAP)

Treat unincorporated dNTPs in PCR reactions to prepare templates for DNA sequencing or SNP analysis. Shrimp Alkaline Phosphatase (SAP) is useful in molecular biology applications such as the dephosphorylation of phosphorylated ends of DNA or RNA for subsequent use in cloning or end-labeling of probes.

Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

An enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by western blot analysis

Thermo Scientific™ PVDF Transfer Membrane, 0.45 µm, 26.5 cm x 3.75 m

Enable high protein and nucleic acid binding capacity with sheets and rolls of high-quality polyvinylidene difluoride (PVDF) membrane.

Thermo Scientific™ Western Blot Roller

A simple, reusable tool for gently and easily removing trapped air bubbles when assembling transfer stacks in semi-dry or wet-tank transfer cassettes.

Thermo Scientific™ Pierce™ Protein-Free (PBS) Blocking Buffer

Block membranes, plates and other samples with these ready-to-use PBS and TBS formulations of a non-protein compound to yield extremely low background.

Invitrogen™ Invitrolon™ PVDF/Filter Paper Sandwiches, 0.45 µm, 8.3 x 7.3 cm (for mini gels)

Pre-cut and pre-assembled and ready for protein blotting/transfer

Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.

Invitrogen™ Mini Gel Tank and Blot Module Set

Provides all of the necessary devices for protein separation and Western blotting using Novex™ gels, NuPAGE™ gels, and Bolt™ Bis-Tris Plus gels

Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate

Luminol-based enhanced chemiluminescence (ECL) HRP substrate with very stable light output for femtogram-level detection with CCD-based imagers.

Invitrogen™ iBlot™ Transfer Stack, nitrocellulose, regular size

iBlot Transfer Stacks are used to transfer proteins

Invitrogen™ CellROX™ Orange Reagent, for oxidative stress detection

Novel fluorogenic probe for measuring oxidative stress in live cells

Thermo Scientific™ Blocker™ BLOTTO in TBS

Block membranes, plates and other samples with this ready-to-use 5% solution of nonfat powdered milk in TBS for Western blot, ELISA and other detection methods.

Thermo Scientific™ Lab Vision™ UltraVision™ LP Detection System: HRP Polymer (Ready-To-Use)

Employ a senstive detection system based on biotin-free, polymer technology.

Cytiva Amersham™ ECL™ Western Blotting Detection Reagents

Use for all routine Western blotting. Cytiva Life Sciences™ Amersham™ ECL™ Western Blotting Detection Reagents are the world’s first commercially available chemiluminescent detection reagents and still one of the most widely used.

Cytiva Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls

Use this versatile nitrocellulose membrane for excellent binding affinity for small proteins and peptides, as well as nucleic acids. Cytiva Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.

Invitrogen™ iBlot™ 2 Starter Kit

The iBlot 2 Starter kit includes the iBlot 2 Gel Transfer Device as well as iBlot 2 Transfer Stacks (nitrocellulose) - everything needed to perform a rapid dry transfer of proteins from a gel to a membrane.

  spinner