Life Science Buffers
Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
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Filtered Search Results
TBE Buffer, Tris-Borate-EDTA, 10X Solution, Electrophoresis, Fisher BioReagents™
CAS: 10043-35-3 Synonym: TBE
CAS | 10043-35-3 |
---|---|
Synonym | TBE |
TAE Buffer, Tris-Acetate-EDTA, 50X Solution, Electrophoresis, Fisher BioReagents™
Tris-Acetate-EDTA, CAS Number-77-86-1, 60-00-4, 6850-28-8, TAE, 4L, Gray, Tris (24%), Acetic Acid (5.0%), and EDTA (<2%)., DNase free, Pass Test, Filtered through a 0.2-micron filter., Electrophoresis, 50X Solution, Poly CUBE, Liquid, Protease free, DNase-, RNase- and Protease-Free, RT
Name Note | 50X Solution |
---|---|
Concentration or Composition (by Analyte or Components) | Tris (24%), Acetic Acid (5.0%), and EDTA (<2%). |
CAS | 6850-28-8 |
Protease | Protease free |
Physical Form | Liquid |
Grade | Electrophoresis |
Synonym | TAE |
Purity Grade Notes | DNase-, RNase- and Protease-Free |
DNase | DNase free |
Recommended Storage | RT |
Filtered Through | Filtered through a 0.2-micron filter. |
ChemAlert Storage Symbol | Gray |
TE Buffer, Tris-EDTA, 1X Solution, pH 8.0, Molecular Biology, Fisher BioReagents
Color | Colorless |
---|---|
Physical Form | Liquid |
Chemical Name or Material | Tris-EDTA |
Grade | Molecular Biology |
DNase | DNase free |
Filtered Through | Filtered through a 5-micron filter. |
ChemAlert Storage Symbol | Gray |
Name Note | 1X Solution, pH 8.0 |
CAS | 7732-18-5 |
Protease | Protease free |
Health Hazard 3 | Emergency Overview May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Remove from exposure, lie down. Move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. If not breathing, give artificial respiration. Obtain medical attention. NFPA Health:1 Flammability:0 Instability:0 |
Health Hazard 2 | CAUTION! |
pH | 7.4 to 8.1 |
Synonym | TE |
Recommended Storage | RT |
TBS, Tris Buffered Saline, 10X Solution, pH 7.4, Molecular Biology, Fisher BioReagents™
Boiling Point | 100°C |
---|---|
Color | Colorless |
Physical Form | Liquid |
Chemical Name or Material | Tris Buffered Saline |
Grade | Molecular Biology |
DNase | DNase free |
ChemAlert Storage Symbol | Gray |
Name Note | 10X Solution |
Concentration or Composition (by Analyte or Components) | 10X solution contains 1.37M Sodium Chloride, 0.027M Potassium Chloride, and 0.25M Tris/Tris-HCl. |
CAS | 7732-18-5 |
Protease | Protease free |
pH | 7.5 |
Synonym | TBS |
Purity Grade Notes | DNase-, RNase- and Protease-Free |
Recommended Storage | RT |
HEPES Buffer, 1M Solution, pH 7.3 (Molecular Biology), Fisher BioReagents™
Commonly used buffering agent
Color | Undesignated |
---|---|
Physical Form | Liquid |
Chemical Name or Material | HEPES Buffer |
Grade | Molecular Biology |
Identification | Pass Test |
DNase | DNase free |
Merck Index | 15, 4689 |
Concentration | 0.95 to 1.05 M |
ChemAlert Storage Symbol | Gray |
Absorbance | ≤0.01(0.1M solution) at 260nm,0.004 max. (0.1M solution) at 280nm |
Name Note | 1M Solution, pH 7.3 |
CAS | 7732-18-5 |
Protease | Protease free |
pH | 7.2 to 7.5 |
Synonym | N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid |
Purity Grade Notes | DNase-, RNase- and Protease-Free |
Recommended Storage | RT |
Molecular Formula | C8H18N2O4S |
Formula Weight | 238.197 |
PBS Tablets, Phosphate Buffered Saline, Fisher BioReagents™
Phosphate Buffered Saline Tablets, CAS Number-7647-14-5, 7447-40-7, 100 tablets, Clear, Gray, White, 5/8 in., 600 SEC max., CAUTION!
PBS, Phosphate Buffered Saline, 10X Solution, Fisher BioReagents™
Commonly used buffer
Content And Storage | Room temperature |
---|---|
Form | Liquid |
TAE Buffer, Tris-Acetate-EDTA, 10X Solution, Electrophoresis, Fisher BioReagents
Name Note | 10X Solution |
---|---|
Concentration or Composition (by Analyte or Components) | Tris (<5.0%), Acetic Acid (1.0%), and EDTA (<1%). |
CAS | 77-86-1 |
Protease | Protease free |
Physical Form | Liquid |
Chemical Name or Material | Tris-Acetate-EDTA |
Grade | Electrophoresis |
Synonym | TAE |
Purity Grade Notes | DNase-, RNase- and Protease-Free |
DNase | DNase free |
Recommended Storage | RT |
Filtered Through | Filtered through a 0.2-micron filter. |
ChemAlert Storage Symbol | Gray |
TE Buffer, Tris-EDTA, 100X Solution, pH 8.0, Molecular Biology, Fisher BioReagents™
Name Note | 100X Solution |
---|---|
CAS | 7732-18-5 |
Protease | Protease free |
Health Hazard 3 | Emergency Overview Irritating to eyes and skin. Wear personal protective equipment. Ensure adequate ventilation. Avoid contact with skin, eyes and clothing. Avoid ingestion and inhalation. Wash off immediately with plenty of water for at least 15 minutes. Get medical attention immediately if symptoms occur. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Get medical attention immediately if symptoms occur. Get medical attention immediately if symptoms occur. Do not induce vomiting. NFPA Health:2 Flammability:0 Instability:0 |
Health Hazard 2 | WARNING! |
Color | Undesignated |
Physical Form | Liquid |
Chemical Name or Material | Tris-EDTA |
Grade | Molecular Biology |
DNase | DNase free |
Recommended Storage | RT |
Filtered Through | Filtered through a 0.2-micron filter. |
ChemAlert Storage Symbol | Gray |
TBE Buffer, Tris-Borate-EDTA, 1X Solution, Electrophoresis, Fisher BioReagents
Name Note | 1X Solution |
---|---|
CAS | 7732-18-5 |
Protease | Protease free |
Color | Colorless |
Physical Form | Liquid |
pH | 8.2 to 8.4 |
Chemical Name or Material | Tris-Borate-EDTA |
Grade | Electrophoresis |
Synonym | TBE |
DNase | DNase free |
Recommended Storage | RT |
Filtered Through | Filtered through a 0.2-micron filter. |
ChemAlert Storage Symbol | Gray |
TE Buffer, Tris-EDTA, 1X Solution, pH 7.4, Molecular Biology, Fisher BioReagents
Color | Colorless |
---|---|
Physical Form | Liquid |
Chemical Name or Material | Tris-EDTA |
Grade | Molecular Biology |
DNase | DNase free |
Filtered Through | Filtered through a 5-micron filter. |
ChemAlert Storage Symbol | Gray |
Name Note | 1X Solution, pH 7.4 |
CAS | 7732-18-5 |
Protease | Protease free |
Health Hazard 3 | Emergency Overview May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Remove from exposure, lie down. Move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. If not breathing, give artificial respiration. Obtain medical attention. NFPA Health:1 Flammability:0 Instability:0 |
Health Hazard 2 | CAUTION! |
pH | 7.4 to 8.1 |
Synonym | TE |
Recommended Storage | RT |
TBS, Tris Buffered Saline, 1X Solution, pH 7.4, Molecular Biology, Fisher BioReagents
Boiling Point | 100°C |
---|---|
Name Note | 1X Solution, pH 7.4 |
CAS | 77-86-1 |
Protease | Protease free |
Color | Colorless |
Physical Form | Liquid |
Chemical Name or Material | Tris Buffered Saline |
Grade | Molecular Biology |
Synonym | TBS |
DNase | DNase free |
Recommended Storage | RT |
ChemAlert Storage Symbol | Gray |
Content And Storage | Room temperature |
---|---|
Form | Liquid |
Packaging Type | Poly Cube |
pH | 7.3 to 7.5 |
Melting Point | 0°C |