Real Time PCR Reagents and Kits
Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
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Filtered Search Results
Applied Biosystems™ MycoSEQ™ Mycoplasma Detection Kits
The MycoSEQ Mycoplasma Detection Kit is a real-time mycoplasma PCR assay designed and validated to meet the requirements of European Pharmacopeia Chapter 2.6.7 for use as a nucleic acid amplification technique (NAT).
Description | Detection for BioProduction process media, cells, raw materials |
---|---|
Label or Dye | SYBR Green |
Quantity | 100 Reactions |
For Use With (Application) | Mycoplasma Detection |
Product Line | MycoSEQ™ |
TaqMan™ TaqMan™ Advanced miRNA cDNA Synthesis Kit
Kit uses 3' poly-A tailing and 5' ligation of an adaptor sequence to extend the mature miRNAs present in the sample on each end prior to reverse transcription.
TaqMan™ TaqMan™ MicroRNA Reverse Transcription Kit
Provides the necessary components for 200 reactions of optimal performance in TaqMan™ MicroRNA Assays.
Shipping Condition | Dry Ice |
---|---|
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 10 X |
Reverse Transcriptase | MultiScribe™ |
Final Product Type | cDNA |
Concentration | 10X |
For Use With (Application) | miRNA analysis |
PCR Method | 2-step RT-qPCR |
Detection Method | Primer-probe |
Size (Final Product) | 200 bp or less |
Reaction Format | Separate Components |
Product Type | MicroRNA Reverse Transcription Kit |
Sample Type | RNA |
Reaction Speed | Fast |
Optimal Reaction Temperature | 42°C |
Product Line | TaqMan™ |
Applied Biosystems™ RNA UltraSense™ One-Step Quantitative RT-PCR System
RNA UltraSense™ One-Step Quantitative RT-PCR System
Invitrogen™ ROX Reference Dye
ROX Reference Dye is designed to normalize the fluorescent reporter signal in real-time quantitative PCR or RT-PCR.
TaqMan™, RNA-to-CT™ TaqMan™ RNA-to-CT™ 1-Step Kit
Delivers consistent RNA target quantification for a wide variety of assays and is validated with Applied Biosystems™ comprehensive library of TaqMan™ Gene Expression assays.
Polymerase | AmpliTaq Gold DNA Polymerase |
---|---|
Purity or Quality Grade | UP (Ultra Pure) |
Reverse Transcriptase | ArrayScript™ UP |
Product Type | One-Step RT-PCR Kit |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode |
Sample Type | RNA |
Reaction Speed | Standard |
For Use With (Application) | Gene Expression |
PCR Method | 1-step RT-qPCR |
Product Line | RNA-to-CT™, TaqMan™ |
Detection Method | Primer-probe |
Applied Biosystems™ resDNASEQ™ Quantitative E. coli DNA Kits
This quantitative PCR (qPCR)-based kit, part of the resDNASEQ™ E. coli Residual DNA Quantitation System, enables highly sensitive quantitation of host cell DNA from E. coli expression systems, typically in less than 4 hours.
No. of Reactions | 100 Reactions |
---|---|
Format | Kit |
Label or Dye | FAM |
Host Cell | E.coli |
PCR Method | qPCR |
Product Line | resDNASEQ™ |
Detection Method | Primer-probe |
Invitrogen™ SuperScript™ III Platinum™ One-Step qRT-PCR Kit
Combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format.
Shipping Condition | Dry Ice |
---|---|
Polymerase | Taq DNA Polymerase |
Product Type | One-Step qRT-PCR Kit |
For Use With (Equipment) | 7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ |
Sample Type | RNA |
Reaction Speed | Standard |
For Use With (Application) | Gene Expression,Pathogen Detection,Virus Detection |
PCR Method | 1-step RT-qPCR |
Product Line | Platinum™, SuperScript™ |
Detection Method | Primer-probe |
Applied Biosystems™ TaqMan™ Exogenous Internal Positive Control Reagents
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
TaqMan™ Exogenous Internal Positive Control Reagents contain a pre-optimized internal positive control (IPC) with pre-designed primers and TaqMan™ probe.
GC-Rich PCR Performance | Low |
---|---|
Format | Tube(s) |
Internal Probe Modification | TAMRA™ Quencher (3'), VIC™ (5') |
Product Type | Exogenous Internal Positive Control Reagent |
Reaction Speed | Standard |
Green Features | Sustainable packaging |
PCR Method | qPCR |
Product Line | TaqMan™ |
Applied Biosystems™ Power SYBR™ Green RNA-to-CT™ 1-Step Kit
The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.
Shipping Condition | Wet Ice |
---|---|
Purity or Quality Grade | UP (Ultra Pure) |
Reverse Transcriptase | ArrayScript™ UP |
For Use With (Application) | Gene Expression |
PCR Method | 1-step RT-qPCR |
Detection Method | SYBR |
Polymerase | AmpliTaq Gold DNA Polymerase |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System |
Sample Type | RNA |
Reaction Speed | Standard |
Label or Dye | SYBR Green |
Run Time | Standard |
Product Line | RNA-to-CT™, SYBR™ |
Applied Biosystems™ QuantStudio™ Absolute Q™ Isolation Buffer
The QuantStudio Absolute Q Isolation Buffer is intended to supplement the materials supplied in the QuantStudio Absolute Q MAP16 Plate Kit.
Applied Biosystems™ resDNASEQ™ Quantitative CHO DNA Kits
This quantitative PCR (qPCR)-based kit, part of the resDNASEQ™ Quantitative CHO DNA System, allows users to detect sub-picogram amounts of residual DNA from CHO cell line in under 5 hours.
No. of Reactions | 100 Reactions |
---|---|
Format | Kit |
Label or Dye | FAM |
Host Cell | CHO |
PCR Method | qPCR |
Product Line | resDNASEQ™ |
Detection Method | Primer-probe |
Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents
AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.
Shipping Condition | Dry Ice |
---|---|
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 25 X |
Description | Works on all AB platforms, but is optimized for the 7500. |
Reverse Transcriptase | ArrayScript™ |
Concentration | 25X |
PCR Method | 1-step RT-qPCR |
Detection Method | Primer-probe |
Polymerase | AmpliTaq Gold DNA Polymerase |
Format | Tube |
Product Type | One-Step RT-PCR Reagent |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex |
Sample Type | RNA |
Reaction Speed | Standard |
Product Line | AgPath-ID™, Ambion™ |
Applied Biosystems™ resDNASEQ™ Quantitative Plasmid DNA - Kanamycin Resistance Gene Kits
The resDNASEQ Quantitative Plasmid DNA - Kanamycin Resistance Gene Kit is a quantitative PCR (qPCR)-based system for the detection of residual kanamycin-resistant plasmids.
No. of Reactions | 100 Reactions |
---|---|
Target | Kanamycin Resistant Plasmids |
Format | Kit |
Label or Dye | FAM |
PCR Method | qPCR |
Product Line | resDNASEQ™ |
Detection Method | Primer-probe |
SuperScript™ EXPRESS One-Step Superscript™ qRT-PCR Kit, universal
Kit incorporates proven Invitrogen™ enzymes specifically tailored to deliver superior results on high-throughput, fast-cycling instruments for probe-based qPCR experiments.
Polymerase | Taq DNA Polymerase |
---|---|
Format | Tube |
Product Type | qRT-PCR Kit |
For Use With (Equipment) | 7500 Fast System, 7500 System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, BioRad CFX96™, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad MyiQ, Corbett RotorGene, Cepheid SmartCycler, MJ Opticon, MJ Chromo4, BioRad iCycler iQ |
Sample Type | RNA |
Reaction Speed | Fast or Standard |
PCR Method | 1-step RT-qPCR |
Product Line | SuperScript™ |
Detection Method | Primer-probe |