PCR Supplies

PCR Supplies






Thermo Scientific™ Phusion Flash High-Fidelity PCR Master Mix
Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time.

Shipping Condition | Dry Ice |
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Polymerase | Phusion Flash II DNA Polymerase |
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 25X |
Size (Final Product) | 20 kb or less |
Reaction Format | SuperMix or Master Mix |
Product Type | High-Fidelity PCR Master Mix |
Reaction Speed | Fast |
Color | Colorless |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Concentration | 2X |
For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Applied Biosystems™ TaqPath™ 1-Step Multiplex Master Mix (No ROX)
TaqPath 1-Step Multiplex Master Mix (No ROX) is a fast, single-tube, 4X RT-qPCR mix that provides for sensitive, reproducible detection of up to four different RNA/DNA targets in a single multiplex reaction.

Shipping Condition | Dry Ice |
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Format | Tube |
For Use With (Equipment) | QuantStudio™ 6 Pro or QuantStudio™ 7 Pro, QuantStudio™ 3 or 5, QuantStudio™ 6/QuantStudio™ 7 Flex, QuantStudio™ 12K Flex, QuantStudio™ Dx, QuantStudio™ Absolute Q Digital PCR System, ViiA™ 7, 7500, 7500 Fast, 7500 Fast Dx, or use a compatible real-time PCR instrument from another supplier |
Sample Type | DNA, RNA |
Reaction Speed | Fast or Standard |
For Use With (Application) | Gene Expression,Virus Detection |
PCR Method | 1-step RT-qPCR,Multiplex qPCR |
Product Line | TaqPath™ |
Detection Method | Primer-probe |
Invitrogen™ Taq DNA Polymerase, recombinant
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.

Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1X |
Overhang | 3'-A |
Concentration | 5 U/ÎĽL |
For Use With (Application) | Standard PCR |
PCR Method | qPCR,Standard PCR |
Exonuclease Activity | 5' - 3' |
Polymerase | Taq DNA Polymerase |
Format | Stand-alone enzyme |
Size (Final Product) | 5 kb or less |
Reaction Format | Separate Components |
Product Type | DNA Polymerase |
Reaction Speed | Standard |
Hot Start | No |
Starting Material | DNA |
Invitrogen™ SuperScript™ II Reverse Transcriptase
Invitrogen SuperScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) with reduced RNase H activity and increased thermal stability compared to wild-type MMLV RT.

Shipping Condition | Wet Ice |
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GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript II |
Final Product Type | First-Strand cDNA |
Concentration | 200 U/ÎĽL |
Format | Stand-alone Enzyme |
Size (Final Product) | Up to 12.3 kb |
Reaction Format | Separate components |
Reaction Speed | 50 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 42°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Applied Biosystems™ TaqMan™ Fast Virus 1-Step Master Mix for qPCR
TaqMan Fast Virus 1-Step Master Mix is designed for fast, highly sensitive real-time RT-PCR, even in the presence of challenging PCR inhibitors.

Shipping Condition | Dry Ice |
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Reverse Transcriptase | M-MLV |
Form | Liquid |
For Use With (Application) | Gene Expression,Virus Detection |
PCR Method | 1-step RT-qPCR |
Detection Method | Primer-probe |
Polymerase | AmpliTaq Fast DNA Polymerase |
Format | Tube |
Product Type | 1-Step Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 7 Flex, QuantStudio™ Absolute Q Digital PCR System, StepOne™, StepOnePlus™, ViiA™ 7 System |
Sample Type | Total RNA, Viral DNA, Viral RNA, mRNA |
Reaction Speed | Fast or Standard |
Hot Start | Built-In Hot Start |
Product Line | TaqMan™ |
Applied Biosystems™ TaqPath™ 1-Step RT-qPCR Master Mix, CG
TaqPath 1-Step RT-qPCR Master Mix, CG, is designed for robust and reproducible one-step pathogen detection and gene expression workflows. The single-tube 4X formulation contains thermostable MMLV reverse transcriptase, dNTPs, UNG, ROX dye, and thermostable Fast DNA polymerase.

Polymerase | Fast DNA Polymerase |
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Reverse Transcriptase | M-MLV |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System, 7500 Fast Dx System, QuantStudio™ Dx |
Form | Liquid |
Sample Type | RNA, cDNA, DNA |
Reaction Speed | Fast or Standard |
Hot Start | Built-In Hot Start |
Concentration | 4X |
For Use With (Application) | Gene Expression,Virus Detection |
PCR Method | 1-step RT-qPCR |
Product Line | TaqPath™ |
Detection Method | Primer-probe |
Thermo Scientific™ Maxima H Minus Reverse Transcriptase (200 U/μL)
Thermo Scientific Maxima H Minus Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity but lacks RNase H activity due to mutation in RNase H domain of M-MuLV RT.

Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Reverse Transcriptase | Maxima H Minus |
Final Product Type | First-Strand cDNA |
Concentration | 200 U/ÎĽL |
Format | Stand-alone Enzyme |
Size (Final Product) | Up to 20 kb |
Reaction Format | Separate components |
Reaction Speed | 15 to 30 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 50°C to 55°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR
Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

Shipping Condition | Wet Ice |
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Polymerase | Dual-Lock Taq DNA Polymerase |
Format | Tube |
Product Type | Real Time PCR SYBR Master Mix |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 7 Flex, QuantStudio™ Absolute Q Digital PCR System, StepOne™, StepOnePlus™, ViiA™ 7 System |
Sample Type | DNA |
Reaction Speed | Fast or Standard |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Product Line | PowerUP™, SYBR™ |
Detection Method | SYBR |
Applied Biosystems™ Absolute Q™ DNA Digital PCR Master Mix (5X)
Applied Biosystems Absolute Q DNA Digital PCR Master Mix (5X) is optimized for use with the QuantStudio Absolute Q Digital PCR System and Applied Biosystems digital PCR (dPCR) assays in a simple workflow with minimal processing steps.

Thermo Scientific™ Phusion High-Fidelity PCR Master Mix with HF or GC Buffer
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Shipping Condition | Dry Ice |
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Polymerase | Phusion High-Fidelity DNA Polymerase |
Fidelity (vs. Taq) | 52X |
Size (Final Product) | 20 kb or less |
Reaction Format | SuperMix or Master Mix |
Product Type | High-Fidelity PCR Master Mix |
Reaction Speed | Fast |
Hot Start | No |
Overhang | Blunt |
Concentration | 2X |
For Use With (Application) | Standard PCR,High-fidelity PCR |
Applied Biosystems™ TaqMan™ Environmental Master Mix 2.0
Accurate, real-time PCR-based pathogen detection in the presence of high levels of inhibitors.

Purity or Quality Grade | UP (Ultra Pure) |
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Concentration | 2X |
For Use With (Application) | Gene Expression,Genotyping,Pathogen Detection,Virus Detection |
PCR Method | qPCR |
Detection Method | Primer-probe |
Polymerase | AmpliTaq Gold DNA Polymerase |
Format | Bottle |
Product Type | qPCR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7000 System, 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOne™, Standard Mode |
Sample Type | Environmental Sample, Food |
Reaction Speed | Standard |
Hot Start | Built-In Hot Start |
Product Line | TaqMan™ |
Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Gold Buffer and MgCl2
AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.

Polymerase | AmpliTaq Gold DNA Polymerase |
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GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1X |
Size (Final Product) | 5 kb or less |
Reaction Format | Separate Components |
Product Type | DNA Polymerase |
Hot Start | Built-In Hot Start |
Overhang | 3'-A |
Concentration | 5 U/ÎĽL |
Exonuclease Activity | 5' - 3' |
Thermo Scientific™ Phusion™ Plus PCR Master Mixes
Thermo Scientific Phusion Plus PCR Master Mix is a convenient, ready-to-use 2X mixture of Phusion Plus DNA Polymerase, buffer, magnesium chloride, and dNTPs.

Shipping Condition | Dry Ice |
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Polymerase | Phusion Plus DNA Polymerase |
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | >100X |
Size (Final Product) | 20 kb or less |
Reaction Format | Ready to use master mix |
Reaction Speed | 15 to 30 sec./kb |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Concentration | 2X |
For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ dATP Solution (100 mM)
Thermo Scientific dATP (2'-deoxyadenosine 5'-triphosphate) is highly stable nucleotide, supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.

Invitrogen™ SuperScript™ IV VILO™ Master Mix
SuperScript IV VILO (SSIV VILO) Master Mix, available with or without ezDNase, is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications.

Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript IV |
Final Product Type | First-Strand cDNA |
Quantity | Each |
Concentration | 5X |
For Use With (Application) | Real Time PCR (qPCR) |
Detection Method | qPCR |
Format | Master Mix |
Size (Final Product) | Up to 10 kb |
Reaction Format | Master Mix |
Reaction Speed | 10 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 50°C |
Technique | Reverse Transcription |
Starting Material | RNA |