
PCR Equipment and Supplies

Thermo Scientificâ„¢ Phusion Flash High-Fidelity PCR Master Mix
Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time.

Shipping Condition | Dry Ice |
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Polymerase | Phusion Flash II DNA Polymerase |
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 25X |
Size (Final Product) | 20 kb or less |
Reaction Format | SuperMix or Master Mix |
Product Type | High-Fidelity PCR Master Mix |
Reaction Speed | Fast |
Color | Colorless |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Invitrogenâ„¢ SuperScriptâ„¢ II Reverse Transcriptase
Invitrogen SuperScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) with reduced RNase H activity and increased thermal stability compared to wild-type MMLV RT.

Shipping Condition | Wet Ice |
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GC-Rich PCR Performance | High |
Format | Stand-alone Enzyme |
Reverse Transcriptase | SuperScript II |
Size (Final Product) | Up to 12.3 kb |
Reaction Format | Separate components |
Final Product Type | First-Strand cDNA |
Reaction Speed | 50 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 42°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Applied Biosystemsâ„¢ Region of Interest (ROI) and Background Plates, 96-well
Used to maintain the ViiAâ„¢ 7, QuantStudioâ„¢ 3, QuantStudioâ„¢ 5, QuantStudioâ„¢ 6, QuantStudioâ„¢ 7, and QuantStudioâ„¢ 12K Flex real-time PCR systems with 96-well (0.2 mL) blocks.

Applied Biosystems™ Random Hexamers (50 μM)
Random Hexamers are short oligodeoxyribonucleotides of random sequence [d(N)6] that anneal to random complementary sites on a target DNA or RNA to serve as primers for DNA synthesis by a DNA polymerase or reverse transcriptase.

Thermo Scientificâ„¢ Exo-Resistant Random Primer
Thermo Scientific Exonuclease-Resistant Random Primer is a mixture of single-stranded random oligonucleotides used for highly efficient random priming of various DNA synthesis reactions.

Applied Biosystemsâ„¢ TaqManâ„¢ Fast Virus 1-Step Master Mix for qPCR
TaqMan Fast Virus 1-Step Master Mix is designed for fast, highly sensitive real-time RT-PCR, even in the presence of challenging PCR inhibitors.

Shipping Condition | Dry Ice |
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Reverse Transcriptase | M-MLV |
Form | Liquid |
For Use With (Application) | Gene Expression,Virus Detection |
PCR Method | 1-step RT-qPCR |
Detection Method | Primer-probe |
Polymerase | AmpliTaq Fast DNA Polymerase |
Format | Tube |
Product Type | 1-Step Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudioâ„¢ 12k Flex, QuantStudioâ„¢ 3, QuantStudioâ„¢ 5, QuantStudioâ„¢ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudioâ„¢ 7 Flex, QuantStudioâ„¢ Absolute Q Digital PCR System, StepOneâ„¢, StepOnePlusâ„¢, ViiAâ„¢ 7 System |
Sample Type | Total RNA, Viral DNA, Viral RNA, mRNA |
Reaction Speed | Fast or Standard |
Hot Start | Built-In Hot Start |
Product Line | TaqManâ„¢ |
Fisherbrandâ„¢ SYBRâ„¢ Green qPCR Master Mix
Fisherbrandâ„¢ SYBRâ„¢ Green qPCR Master Mix is a 2X, ready-to-use master mix designed for dye-based, quantitative amplification of DNA (cDNA and gDNA) targets by real-time PCR.

Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Content And Storage | SYBR Green dye, DNA polymerase, with an antibody-mediated hot start, Heat-labile uracil-DNA glycosylase (UDG), ROX dye (passive reference dye), dNTP blend containing dUTP/dTTP, Optimized buffer components; Store at -20°C ± 10°C in the dark |
Product Type | Master Mix |
Form | Frozen |
Reaction Speed | Fast or Standard |
Label or Dye | SYBR Green,FAM |
For Use With (Application) | Gene Expression,DNA Quantitation,Microbial Detection,Real Time PCR (qPCR) |
PCR Method | qPCR,2-step RT-qPCR,RT-qPCR |
Detection Method | SYBR |
Applied Biosystemsâ„¢ PowerUpâ„¢ SYBRâ„¢ Green Master Mix for qPCR
Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

Shipping Condition | Wet Ice |
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Polymerase | Dual-Lock Taq DNA Polymerase |
Format | Tube |
Product Type | Real Time PCR SYBR Master Mix |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudioâ„¢ 12k Flex, QuantStudioâ„¢ 3, QuantStudioâ„¢ 5, QuantStudioâ„¢ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudioâ„¢ 7 Flex, QuantStudioâ„¢ Absolute Q Digital PCR System, StepOneâ„¢, StepOnePlusâ„¢, ViiAâ„¢ 7 System |
Sample Type | DNA |
Reaction Speed | Fast or Standard |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Product Line | PowerUPâ„¢, SYBRâ„¢ |
Detection Method | SYBR |
Thermo Scientificâ„¢ dNTP Set, 100 mM Solutions
Thermo Scientific dNTP Set is a convenient set of 100 mM aqueous solutions of each of dATP, dCTP, dGTP and dTTP, supplied in separate vials.

Shipping Condition | Dry Ice |
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Purity or Quality Grade | >99% |
Form | Liquid |
Label or Dye | Unlabeled |
For Use With (Application) | Standard PCR,High-fidelity PCR,Real Time PCR (qPCR) |
Applied Biosystemsâ„¢ Absolute Qâ„¢ DNA Digital PCR Master Mix (5X)
Applied Biosystems Absolute Q DNA Digital PCR Master Mix (5X) is optimized for use with the QuantStudio Absolute Q Digital PCR System and Applied Biosystems digital PCR (dPCR) assays in a simple workflow with minimal processing steps.

Thermo Scientificâ„¢ Phire Tissue Direct PCR Master Mix
Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.

Shipping Condition | Wet or Dry Ice |
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Polymerase | Phire Hot Start II DNA Polymerase |
GC-Rich PCR Performance | Medium |
Includes Label or Dye | Yes |
Reaction Format | SuperMix or Master Mix |
Product Type | Direct PCR Master Mix |
Sample Type | Tissue |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Starting Material | DNA |
For Use With (Application) | Genotyping,Hot-start PCR |
PCR Method | Direct PCR |
Applied Biosystemsâ„¢ TaqPathâ„¢ 1-Step Multiplex Master Mix (No ROX)
TaqPath 1-Step Multiplex Master Mix (No ROX) is a fast, single-tube, 4X RT-qPCR mix that provides for sensitive, reproducible detection of up to four different RNA/DNA targets in a single multiplex reaction.

Shipping Condition | Dry Ice |
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Format | Tube |
For Use With (Equipment) | QuantStudioâ„¢ 6 Pro or QuantStudioâ„¢ 7 Pro, QuantStudioâ„¢ 3 or 5, QuantStudioâ„¢ 6/QuantStudioâ„¢ 7 Flex, QuantStudioâ„¢ 12K Flex, QuantStudioâ„¢ Dx, QuantStudioâ„¢ Absolute Q Digital PCR System, ViiAâ„¢ 7, 7500, 7500 Fast, 7500 Fast Dx, or use a compatible real-time PCR instrument from another supplier |
Sample Type | DNA, RNA |
Reaction Speed | Fast or Standard |
For Use With (Application) | Gene Expression,Virus Detection |
PCR Method | 1-step RT-qPCR,Multiplex qPCR |
Product Line | TaqPathâ„¢ |
Detection Method | Primer-probe |
Thermo Scientificâ„¢ Phire Plant Direct PCR Master Mix
Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples.

Shipping Condition | Wet or Dry Ice |
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Polymerase | Phire Hot Start II DNA Polymerase |
GC-Rich PCR Performance | Medium |
Reaction Format | SuperMix or Master Mix |
Product Type | Direct PCR Master Mix |
Sample Type | Plant |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Starting Material | DNA |
For Use With (Application) | Genotyping,Hot-start PCR |
PCR Method | Direct PCR |
Thermo Scientificâ„¢ Phusion High-Fidelity PCR Master Mix with HF or GC Buffer
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Shipping Condition | Dry Ice |
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Polymerase | Phusion High-Fidelity DNA Polymerase |
Fidelity (vs. Taq) | 52X |
Size (Final Product) | 20 kb or less |
Reaction Format | SuperMix or Master Mix |
Product Type | High-Fidelity PCR Master Mix |
Reaction Speed | Fast |
Hot Start | No |
Overhang | Blunt |
For Use With (Application) | Standard PCR,High-fidelity PCR |
Applied Biosystemsâ„¢ TaqPathâ„¢ 1-Step RT-qPCR Master Mix, CG
TaqPath 1-Step RT-qPCR Master Mix, CG, is designed for robust and reproducible one-step pathogen detection and gene expression workflows. The single-tube 4X formulation contains thermostable MMLV reverse transcriptase, dNTPs, UNG, ROX dye, and thermostable Fast DNA polymerase.

Polymerase | Fast DNA Polymerase |
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Reverse Transcriptase | M-MLV |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudioâ„¢ 12k Flex, QuantStudioâ„¢ 3, QuantStudioâ„¢ 5, QuantStudioâ„¢ 6 Flex, QuantStudioâ„¢ 7, StepOneâ„¢, StepOnePlusâ„¢, ViiAâ„¢ 7 System, 7500 Fast Dx System, QuantStudioâ„¢ Dx |
Form | Liquid |
Sample Type | RNA, cDNA, DNA |
Reaction Speed | Fast or Standard |
Hot Start | Built-In Hot Start |
For Use With (Application) | Gene Expression,Virus Detection |
PCR Method | 1-step RT-qPCR |
Product Line | TaqPathâ„¢ |
Detection Method | Primer-probe |