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The Pierce BCA Protein Assay Kit is a high-precision, detergent-compatible protein assay for determination of protein concentration. Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research.
Assay | BCA Assay |
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For Use With (Equipment) | Spectrophotometer, Microplate Reader |
Product Type | Protein Quantitation Assay |
For Use With (Application) | Solution-based Detection, Absorbance |
Product Line | Pierce™ |
Detection Method | Colorimetric |
High-quality, calibrated BSA solutions (2 mg/mL) to serially dilute to create protein assay standard curves to accurately measure protein concentration.
Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.
Substrate Properties | Chemical Substrate |
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Product Type | ELISA Substrate |
Substrate Type | HRP (Horseradish Peroxidase) |
Detection Method | Colorimetric |
Special 3-component version of our popular BCA Reagents to measure total protein concentration of dilute protein solutions (0.5 to 20 micrograms/mL).
Brown-to-purple (A595nm), ready-to-use, classic reducing agent-compatible assay reagent to measure total protein concentration vs. protein standard.
Protein Family | Avidin |
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Content And Storage | Upon receipt store product 4°C. Product is shipped at ambient temperature. |
Target Molecule | Biotin |
Form | Solid |
Label or Dye | Unlabeled |
Ready-to-use, 8M solution of guanidinium hydrochloride (GdnCl, GuHCl), CAS 50-01-1; dilute and pH-adjust to any concentration for protein biology methods.
Estimate visually, or measure with a standard spectrophotometer or plate reader at 562 nm.
Assay | BCA Assay |
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Specificity | Not Target-Specific |
Content And Storage | Store at room temperature. |
Description | Pierce BCA Protein Assay Reagent A |
For Use With (Equipment) | Spectrophotometer, Microplate Reader |
Product Type | Protein Quantitation Assay |
For Use With (Application) | Solution-based Detection, Absorbance |
Product Line | Pierce™ |
Detection Method | Colorimetric |
Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers.
Green-to-blue (A562nm), two-component, precise, detergent-compatible assay reagent to measure total protein concentration vs. protein standard.
High-quality, calibrated BSA solutions (2mg/mL) to serially dilute to create protein assay standard curves to accurately measure protein concentration.
Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.
Shipping Condition | Wet Ice |
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Content And Storage | Upon receipt store desiccated at -20°C. |
Description | EDC |
Cleavable | No |
Water Soluble | Yes |
Form | Powder |
Molecular Weight (g/mol) | 191.7 |
Solubility | Water |
Labeling Method | Chemical Labeling |
Crosslinker Type | Heterobifunctional |
Chemical Reactivity | Amine-Carboxyl |
Cell Permeability | No |
Format | Standard, Single-use, Premium-grade |
Product Type | Crosslinker |
Spacer | Short (<10 AĚŠ) |
Reactive Moiety | Carbodiimide |
Spacer Arm Length | 0.0 AĚŠ |
PEGylated | No |
Reduce protein and peptide disulfide bonds using this thiol-free, pure, crystalline tris (2-carboxyethyl) phosphine hydrochloride, TCEP (CAS 5961-85-3).
Form | Solid |
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Thermo Scientific™ Pierce™ DTT (Dithiothreitol) No-Weigh™ Format is designed for convenient preparation of stocks that will be added to protein samples for effective reduction of disulfide bonds.