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Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrapâ„¢ HP Prepacked Columns are prepacked with Ni Sepharoseâ„¢ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
Flow Rate | 4 mL/min. (1 mL), 5 mL/min. (5 mL) |
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Type | Pre-packed Column |
Content And Storage | 4°C to 30°C |
Description | HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery. |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 3 to 12 |
Material | Polypropylene |
Support Type | 6% Highly Cross-linked Agarose |
Chromogenic Endotoxin Quant Kits are highly sensitive endpoint assays that measure and detect endotoxin (LPS) in protein, peptide, nucleic acid, or antibody samples using the Amebocyte Lysate Assay from 0.01–1.0 EU/mL. Kits are available in reaction sizes ranging from 30 to 240.
Shipping Condition | Wet Ice |
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Assay | End-point Chromogenic Assay |
Type | Endotoxin Quantitation Kit |
Target | Endotoxins |
Content And Storage | • Lyophilized amebocyte lysate • E.coli endotoxin standard • Chromogenic substrate • Endotoxin-free water Store at 2–8°C upon receipt. |
Format | Kit |
Assay Sensitivity | 0.01 EU/mL |
Assay Range | 0.01 to 0.1 EU/mL, 0.1 to 1.0 EU/mL |
Product Line | Pierceâ„¢ |
Detection Method | Colorimetric |
Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciencesâ„¢ HisTrapâ„¢ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharoseâ„¢ 6 Fast Flow.
Flow Rate | <20 mL/min. |
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Type | Pre-packed Column |
Content And Storage | 4°C to 30°C, 20% Ethanol |
Diameter (English) Inner | 0.6 in. |
Description | HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 3 to 12 |
Diameter (Metric) Inner | 16 mm |
Material | Polypropylene |
Support Type | 6% Cross-linked Agarose |
Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 0.5mL. Cytiva Life Sciencesâ„¢ D MiniTrapâ„¢ G-25 Sample Preparation Columns are convenient and prepacked.
For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescienceâ„¢ Superdexâ„¢ 200 Increase 10/300 GL Prepacked Tricornâ„¢ Columns are designed for small scale preparative purification.
Thermo Scientificâ„¢ Pierce Protease Inhibitor Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments
Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.
Buffer | Lysis Buffer |
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Content And Storage | Upon receipt store at 4°C |
Inhibitors | Protease Inhibitor |
Form | Liquid |
Product Type | Protease Inhibitor Cocktail |
Reagent Type | Protease Inhibitor |
Product Line | Haltâ„¢ |
The DynaMag-2 magnet combines a strong magnetic attraction for efficient separations in standard microcentrifuge tubes with a flexible and smart ergonomic design.
Degrade unwanted bacterial cell wall in protein or nucleic acid extracts
Strep-Tactinâ„¢XT 4Flowâ„¢ resin is a 50% suspension to purify Strep-tagâ„¢II or Twin-Strep-tagâ„¢ fusion proteins
Immobilize polyclonal antibodies or other glycoproteins, via periodate-oxidized sugars, onto hydrazide-activated polyacrylamide beads for AP, IP or pull-down assays.
Dynabeads Protein G beads are uniform, 2.8-μm superparamagnetic beads with recombinant Protein G (∼17 kDa) covalently coupled to the surface.
Shipping Condition | Ambient temperature |
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Content And Storage | Dynabeads Protein G are supplied in PBS, pH 7.4 w/0.01% Tween-20, 0.09% NaN3 Store at 2–8°C. |
Purity or Quality Grade | For Research Use Only |
Description | Recombinant Protein G covalently bound to Dynabeads |
Surface Functionality | Protein G |
Material | Polystyrene |
Concentration | 30 mg/mL |
For Use With (Application) | Immunoprecipitation |
High-throughput Compatibility | High-throughput Compatible |
Ligand Type | Protein G |
Type | Superparamagnetic Beads |
Target | Antibodies |
Format | Beads in suspension |
For Use With (Equipment) | KingFisherâ„¢ Sample Purification System, DynaMagâ„¢ magnets |
Diameter (Metric) | 2.8 μm |
Certifications/Compliance | ISO9001 and ISO13485 |
Uniformity | Monosized 2.8 mm (CV <5%) |
Product Line | Dynabeadsâ„¢ |
Purify or immunoprecipitate biotinylated proteins or affinity ligands using streptavidin-coated magnetic particles for magnet-based separation.
Particle Size | 1 μm |
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Ligand Type | Streptavidin |
Type | Magnetic Affinity Bead |
Target | Biotin |
Content And Storage | Upon receipt store at 4°C. Product is shipped on an ice pack. |
Protein Form | Recombinant |
Form | Liquid |
Concentration | Slurry: 10 mg/mL, 1% solids |
Product Line | Pierceâ„¢ |
High-resolution size-exclusion chromatography for studying large proteins and complexes. Cytiva Lifescienceâ„¢ Superoseâ„¢ 6 Increase Columns delivers high quality in size exclusion chromatography (SEC).
Particle Size | 8.6μm |
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Ligand Type | Cross-linked agarose |
Content And Storage | 4° to 30° in 20% ethanol |
pH Range | 3 to 12 |
Column Type | Gel Filtration Column |
Material | Borosilicate glass |
Max. Pressure | 435 psi |
Dynabeads Protein A beads are uniform, 2.8-μm superparamagnetic beads with recombinant Protein A (∼45 kDa) covalently coupled to the surface.
Shipping Condition | Ambient temperature |
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Content And Storage | Dynabeads Protein A are supplied in PBS, pH 7.4 w/0.01% Tween-20, 0.09% NaN3 Store at 2–8°C. |
Purity or Quality Grade | For Research Use Only |
Description | Recombinant Protein A covalently bound to Dynabeads |
Surface Functionality | Protein A |
Material | Polystyrene |
Concentration | 30 mg/mL |
For Use With (Application) | Immunoprecipitation |
High-throughput Compatibility | High-throughput Compatible |
Ligand Type | Protein A |
Type | Superparamagnetic Beads |
Target | Antibodies |
Format | Beads in suspension |
For Use With (Equipment) | KingFisherâ„¢ Sample Purification System, DynaMagâ„¢ magnets |
Diameter (Metric) | 2.8 μm |
Certifications/Compliance | ISO9001 and ISO13485 |
Uniformity | Monosized 2.8 mm (CV <5%) |
Product Line | Dynabeadsâ„¢ |