Protein Purification

Kits, columns, gels, reagents, enzymes, inhibitors, and other assorted products for use with protein purification procedures.

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Cytiva HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

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Specifications

Flow Rate	4 mL/min. (1 mL), 5 mL/min. (5 mL)
Type	Pre-packed Column
Content And Storage	4°C to 30°C
Description	HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Max. Operating Pressure	5 bar (0.5 MPa, 70 psi)
pH Range	3 to 12
Material	Polypropylene
Support Type	6% Highly Cross-linked Agarose

Cytiva Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescience™ Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.

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Thermo Scientific™ Pierce™ Chromogenic Endotoxin Quant Kit

Chromogenic Endotoxin Quant Kits are highly sensitive endpoint assays that measure and detect endotoxin (LPS) in protein, peptide, nucleic acid, or antibody samples using the Amebocyte Lysate Assay from 0.01–1.0 EU/mL. Kits are available in reaction sizes ranging from 30 to 240.

Pricing and Availability
Specifications

Shipping Condition	Wet Ice
Assay	End-point Chromogenic Assay
Type	Endotoxin Quantitation Kit
Target	Endotoxins
Content And Storage	• Lyophilized amebocyte lysate • E.coli endotoxin standard • Chromogenic substrate • Endotoxin-free water Store at 2–8°C upon receipt.
Format	Kit
Assay Sensitivity	0.01 EU/mL
Assay Range	0.01 to 0.1 EU/mL, 0.1 to 1.0 EU/mL
Product Line	Pierce™
Detection Method	Colorimetric

Cytiva HisTrap™ FF Columns: 16 x 25mm

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

Pricing and Availability
Specifications

Flow Rate	<20 mL/min.
Type	Pre-packed Column
Content And Storage	4°C to 30°C, 20% Ethanol
Diameter (English) Inner	0.6 in.
Description	HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
Max. Operating Pressure	5 bar (0.5 MPa, 70 psi)
pH Range	3 to 12
Diameter (Metric) Inner	16 mm
Material	Polypropylene
Support Type	6% Cross-linked Agarose

Cytiva PD MiniTrap™ G-25 Sample Preparation Columns

Use for cleanup of biomolecules with a molecular weight >5000 M_r in sample volumes up to 0.5mL. Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.

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Cytiva Superose™ 6 Increase Columns

High-resolution size-exclusion chromatography for studying large proteins and complexes. Cytiva Lifescience™ Superose™ 6 Increase Columns delivers high quality in size exclusion chromatography (SEC).

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Specifications

Particle Size	8.6μm
Ligand Type	Cross-linked agarose
Content And Storage	4° to 30° in 20% ethanol
pH Range	3 to 12
Column Type	Gel Filtration Column
Material	Borosilicate glass
Max. Pressure	435 psi

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Thermo Scientific™ Pierce™ Protease Inhibitor Mini Tablets, EDTA-free

Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments

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Thermo Scientific™ Halt™ Protease Inhibitor Cocktail (100X)

Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.

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Specifications

Buffer	Lysis Buffer
Content And Storage	Upon receipt store at 4°C
Inhibitors	Protease Inhibitor
Form	Liquid
Product Type	Protease Inhibitor Cocktail
Reagent Type	Protease Inhibitor
Product Line	Halt™

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Invitrogen™ DynaMag™-2 Magnet

The DynaMag-2 magnet combines a strong magnetic attraction for efficient separations in standard microcentrifuge tubes with a flexible and smart ergonomic design.

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Thermo Scientific™ Lysozyme

Degrade unwanted bacterial cell wall in protein or nucleic acid extracts

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Thermo Scientific™ UltraLink™ Hydrazide Resin

Immobilize polyclonal antibodies or other glycoproteins, via periodate-oxidized sugars, onto hydrazide-activated polyacrylamide beads for AP, IP or pull-down assays.

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Invitrogen™ DynaMag™- Spin Magnet

DynaMag-Spin magnet is optimized for efficient magnetic separation of all types of Dynabeads (range 1–4.5 μm in diameter) in small sample volumes (1.5 mL) in microcentrifuge tubes.

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Invitrogen™ Dynabeads™ Protein G for Immunoprecipitation

Dynabeads Protein G beads are uniform, 2.8-μm superparamagnetic beads with recombinant Protein G (∼17 kDa) covalently coupled to the surface.

Pricing and Availability
Specifications

Shipping Condition	Ambient temperature
Content And Storage	Dynabeads Protein G are supplied in PBS, pH 7.4 w/0.01% Tween-20, 0.09% NaN₃ Store at 2–8°C.
Purity or Quality Grade	For Research Use Only
Description	Recombinant Protein G covalently bound to Dynabeads
Surface Functionality	Protein G
Material	Polystyrene
Concentration	30 mg/mL
For Use With (Application)	Immunoprecipitation
High-throughput Compatibility	High-throughput Compatible
Ligand Type	Protein G
Type	Superparamagnetic Beads
Target	Antibodies
Format	Beads in suspension
For Use With (Equipment)	KingFisher™ Sample Purification System, DynaMag™ magnets
Diameter (Metric)	2.8 μm
Certifications/Compliance	ISO9001 and ISO13485
Uniformity	Monosized 2.8 mm (CV <5%)
Product Line	Dynabeads™

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Invitrogen™ Dynabeads™ Protein A for Immunoprecipitation

Dynabeads Protein A beads are uniform, 2.8-μm superparamagnetic beads with recombinant Protein A (∼45 kDa) covalently coupled to the surface.

Pricing and Availability
Specifications

Shipping Condition	Ambient temperature
Content And Storage	Dynabeads Protein A are supplied in PBS, pH 7.4 w/0.01% Tween-20, 0.09% NaN₃ Store at 2–8°C.
Purity or Quality Grade	For Research Use Only
Description	Recombinant Protein A covalently bound to Dynabeads
Surface Functionality	Protein A
Material	Polystyrene
Concentration	30 mg/mL
For Use With (Application)	Immunoprecipitation
High-throughput Compatibility	High-throughput Compatible
Ligand Type	Protein A
Type	Superparamagnetic Beads
Target	Antibodies
Format	Beads in suspension
For Use With (Equipment)	KingFisher™ Sample Purification System, DynaMag™ magnets
Diameter (Metric)	2.8 μm
Certifications/Compliance	ISO9001 and ISO13485
Uniformity	Monosized 2.8 mm (CV <5%)
Product Line	Dynabeads™

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Thermo Scientific™ Pierce™ Streptavidin Magnetic Beads

Purify or immunoprecipitate biotinylated proteins or affinity ligands using streptavidin-coated magnetic particles for magnet-based separation.

Pricing and Availability
Specifications

Particle Size	1 μm
Ligand Type	Streptavidin
Type	Magnetic Affinity Bead
Target	Biotin
Content And Storage	Upon receipt store at 4°C. Product is shipped on an ice pack.
Protein Form	Recombinant
Form	Liquid
Concentration	Slurry: 10 mg/mL, 1% solids
Product Line	Pierce™

Protein Purification

Protein Purification

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