Real Time PCR Reagents and Kits

Real Time PCR Reagents and Kits

Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.

Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.

In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.

A typical RT-PCR kit includes the main components needed for the assay:

  • Buffer
  • Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
  • Reverse transcriptase reagent

 

The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:

    • Fluorogenic primers or labeled fluorogenic probes
    • Fluorescent dye

 

Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.

RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.

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1 – 30 of 136 results
1

Thermo Scientific™ RapidFinder™ Pork ID Kit

The RapidFinder™ Pork ID Kit enables the simple and reliable detection of pork in food and feed samples.

EDGE
2

Applied Biosystems™ Megaplex™ RT Primers, Human Pool Set v3.0

Megaplex™ RT Primers, Human Pool Set v3.0

3

Thermo Scientific™ ABsolute™ qPCR Mixes

ABsolute qPCR Mixes are optimized for use with hybridization probes.

4

Thermo Scientific™ ROX Solution, 50 µM

ROX dye is added to the qPCR reactions to serve as an internal reference for normalization of the fluorescent signal when using real-time instruments that can detect ROX.

5

SYBR™ GreenER™ EXPRESS One-Step SYBR™ GreenER™ Kit, universal

EXPRESS One-Step SYBR GreenER kits are compatible with fast-cycling instruments for your SYBR-based qPCR experiments.

Polymerase Taq DNA Polymerase
Content And Storage

EXPRESS One-Step SYBR™ GreenER™ SuperMix Kits are composed of 2 modules: EXPRESS SYBR™ GreenER™ RT module (containing SuperScript™ III Reverse Transcriptase and RNAseOUT™) and EXPRESS SYBR™ GreenER™ qPCR SuperMix with ROX in a separate tube.

Store at -20°C upon receipt. Once thawed, the EXPRESS SYBR™ GreenER™ qPCR SuperMix can be stored for up to 1 month at +4·C. Product is guaranteed to be stable for 6 months when properly stored.
Format Tube
Product Type One-Step qRT-PCR Kit
For Use With (Equipment) 7500 Fast System, 7500 System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, BioRad CFX96™, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad MyiQ, Corbett RotorGene, Cepheid SmartCycler, MJ Opticon, MJ Chromo4, BioRad iCycler iQ
Reaction Speed Fast, Standard
PCR Method 1-step RT-qPCR
Product Line SYBR™ GreenER™
Detection Method SYBR
6

SuperScript™, Platinum™ SuperScript™ III Platinum™ One-Step qRT-PCR Kit

Combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format.

EDGE
Shipping Condition Dry Ice
Polymerase Taq DNA Polymerase
Product Type One-Step qRT-PCR Kit
For Use With (Equipment) 7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ
Reaction Speed Standard
For Use With (Application) Gene Expression,Pathogen Detection,Virus Detection
PCR Method 1-step RT-qPCR
Product Line Platinum™, SuperScript™
Detection Method Primer-Probe
7

Applied Biosystems™ TaqMan™ Protein Expression Assay Kit (hICAM1)

TaqMan™ Protein Expression Assay Kit (hICAM1)

8

Applied Biosystems™ Megaplex™ Primer Pools, Human Pools A v2.1
GREENER_CHOICE

Megaplex™ Primer Pools, Human Pools A v2.1

9

Applied Biosystems™ Megaplex™ PreAmp Primers, Rodent Pool Set v3.0

Megaplex™ PreAmp Primers, Rodent Pool Set v3.0

10

Applied Biosystems™ TaqMan™ Protein Assays Open Kit

Sensitive Detection — directly measure in vitro protein-protein interactions with only a few thousand cells.

11

TaqMan™ TaqMan™ MicroRNA Reverse Transcription Kit

Provides the necessary components for 200 reactions of optimal performance in TaqMan™ MicroRNA Assays.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase MultiScribe™
Product Type MicroRNA Reverse Transcription Kit
Reaction Speed Fast
Optimal Reaction Temperature 42°C
For Use With (Application) miRNA analysis
PCR Method 2-step RT-qPCR
Product Line TaqMan™
Detection Method Primer-Probe
12

Applied Biosystems™ Fluorescent [ROX] NHS Ester for Post Synthesis Labeling of Primer

13

Thermo Scientific™ RapidFinder™ Beef ID Kit

The RapidFinder™ Beef ID Kit enables the simple and reliable detection of beef in food and feed samples.

14

Applied Biosystems™ TaqMan™ EGFR Exon 19 Deletions Assay

TaqMan™ EGFR Exon 19 Deletions Assay

15

Applied Biosystems™ RapidFinder™ STEC Screening Assay

RapidFinder™ STEC Screening Assay

16

TaqMan™, RNA-to-CT™ TaqMan™ RNA-to-CT™ 1-Step Kit

Delivers consistent RNA target quantification for a wide variety of assays and is validated with Applied Biosystems™ comprehensive library of TaqMan™ Gene Expression assays.

Promotions are available
Polymerase AmpliTaq Gold DNA Polymerase
Purity or Quality Grade UP (Ultra Pure)
Reverse Transcriptase ArrayScript™ UP
Product Type One-Step RT-PCR Kit
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
Reaction Speed Standard
For Use With (Application) Gene Expression
PCR Method 1-step RT-qPCR
Product Line RNA-to-CT™, TaqMan™
Detection Method Primer-Probe
17

Thermo Scientific™ RapidFinder™ Halal ID Kit for RT-PCR

Determine the Halal status of food and feeds using this simple, reliable, sensitive PCR detection method for pork DNA

18

MeltDoctor™ MeltDoctor™ HRM Positive Control Kit

Provides nucleic acid controls to demonstrate and troubleshoot High Resolution Melt (HRM) analysis

19

Invitrogen™ EXPRESS One-Step SYBR™ GreenER™ Kit, with premixed ROX

Polymerase Taq DNA Polymerase
Content And Storage

EXPRESS One-Step SYBR™ GreenER™ SuperMix Kits are composed of 2 modules: EXPRESS SYBR™ GreenER™ RT module (containing SuperScript™ III Reverse Transcriptase and RNAseOUT™) and EXPRESS SYBR™ GreenER™ qPCR SuperMix with ROX premixed.

Store at -20°C upon receipt. Once thawed, the EXPRESS SYBR™ GreenER™ qPCR SuperMix can be stored for up to 1 month at +4·C. Product is guaranteed to be stable for 6 months when properly stored.
Format Tube
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System, Eppendorf™ MasterCycler™ RealPlex, StepOne™, Fast Mode, StepOne™, Standard Mode
Reaction Speed Fast, Standard
For Use With (Application) Gene Expression
PCR Method 1-step RT-qPCR
Product Line SYBR™ GreenER™
Detection Method SYBR
20

Applied Biosystems™ TaqMan™ Brucella spp Detection Kit

TaqMan™ Brucella spp. Detection Kit

21

Thermo Scientific™ Verso 1-step RT-qPCR Mix, low ROX

The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures.

GC-Rich PCR Performance High
Format Tube
Product Type 1-Step RT-qPCR Mix
For Use With (Equipment) 7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P
For Use With (Application) Gene Expression,Pathogen Detection
PCR Method 1-step RT-qPCR
Product Line Verso
Detection Method Primer-Probe
22

Thermo Scientific™ Verso 1-step RT-qPCR Kit, SYBR Green, ROX

The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures.

GC-Rich PCR Performance High
Format Tube
Product Type 1-Step RT-qPCR Kit
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
For Use With (Application) Gene Expression,Pathogen Detection
PCR Method 1-step RT-qPCR
Product Line Verso
Detection Method SYBR
23

Thermo Scientific™ Verso 1-step RT-qPCR Mix, ROX

The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures.

GC-Rich PCR Performance High
Format Tube
Product Type 1-Step QRT-PCR Kit w/ROX
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
For Use With (Application) Gene Expression,Pathogen Detection
PCR Method 1-step RT-qPCR
Product Line Verso
Detection Method Primer-Probe
24

Applied Biosystems™ TaqMan™ Array, Format 24

TaqMan™ Array, Format 24

25

Applied Biosystems™ Megaplex™ Primer Pools, Rodent Pools B v3.0

Megaplex™ Primer Pools, Rodent Pools B v3.0

26

Applied Biosystems™ Megaplex™ Primer Pools, Rodent Pools Set v3.0

Megaplex™ Primer Pools, Rodent Pools Set v3.0 is available under a single part number containing both the Megaplex™ RT Primers and Megaplex™ PreAmp Primers, Rodent Pools Sets.

27

Applied Biosystems™ TaqMan™ PreAmp Master Mix Kit

TaqMan™ PreAmp Master Mix Kit contains TaqMan™ PreAmp Master Mix and TaqMan™ Gene Expression Master Mix.

28

SuperScript™, Platinum™, SYBR™ SuperScript™ III Platinum™ SYBR™ Green One-Step qRT-PCR Kit

Combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format.

Shipping Condition Dry Ice
Polymerase Taq DNA Polymerase
Product Type One-Step qRT-PCR Kit
For Use With (Equipment) 7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ
Reaction Speed Standard
For Use With (Application) 1-Step RT-qPCR
PCR Method 1-step RT-qPCR
Product Line Platinum™, SYBR™, SuperScript™
Detection Method SYBR
29

Applied Biosystems™ RNA UltraSense™ One-Step Quantitative RT-PCR System

RNA UltraSense™ One-Step Quantitative RT-PCR System

30

Platinum™, ThermoScript™ Platinum™ Quantitative RT-PCR ThermoScript™ One-Step System

Platinum Quantitative RT-PCR ThermoScript One-Step System is for real-time quantitation of RNA molecules from total or poly(A)+ RNA in a single step.

Shipping Condition Dry Ice
Polymerase Taq DNA Polymerase
Content And Storage Platinum™ Quantitative RT-PCR ThermoScript™ One-Step System contains ThermoScript™ RT/Platinum™ Taq mix, and 2X ThermoScript™ reaction mix (a buffer containing 0.4 mM of each dNTP, 6 mM MgSO4 ). A vial of 50 mM MgSO4 is also provided. Store at -20°C. Guaranteed stable for 6 months when properly stored.
Product Type RT-PCR One-Step System
Reaction Speed Standard
PCR Method 1-step RT-qPCR
Product Line Platinum™, ThermoScript™
Detection Method Primer-Probe