PCR and qPCR

Thermo Scientific™ ABgene™ Thermo-Fast™ Robotic 96-Well Low Profile PCR Plates

96-well full-skirted SuperPlate which provides 4x more rigidity for superior robotic handling in PCR and qPCR applications. X50 THERMOFAST 96 LP ROBOTIC

Fisherbrand™ 0.2mL PCR Tubes

With integral “snap shut” cap X1000 Fisherbrand PCR tube 0.2ml, domed cap, PP, red - FB-0337/R

Invitrogen™ SuperScript™ III Reverse Transcriptase

Proprietary mutant of SuperScript II RT X4 RT kit Store at -20°C

Invitrogen™ M-MLV Reverse Transcriptase

Recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid 200 KU MLV-REVERSE TRANSCRIPTASE STORE AT -20C

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer II and MgCl2

Chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation 250 UNIT AMPLITAQ GOLD DNA POLYMERASE WITH BUFFERII and MgCl2 solution 250units Store at -20 C

Thermo Scientific™ Maxima™ H Minus Reverse Transcriptase

A highly thermostable, high processivity reverse transcriptase lacking RNase H activity. 2000UNIT Maxima® H Minus ReverseTranscriptase

Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

Nucleaic acid purfication PHENOL/CHLOROFORM/ALCOHOL

Thermo Scientific™ Phusion High-Fidelity DNA Polymerase

Get superior performance for cloning and other applications requiring high fidelity with one of the most accurate thermostable DNA polymerases available. PHUSION HF MASTER MIX 500 RXNS

Invitrogen™ PBS Phosphate-Buffered Saline (10X) pH 7.4

Dilute to desired concentration 10X PBS BUFFER, PH 7.4 500 MLMolecular biology grade, Ambion® PBS 10X, pH 7.4

Thermo Scientific™ 96-Well Non-Skirted Plates

Optimize PCR and qPCR applications with these 96-well non-skirted plates, available with black lettering for improved sample tracking during pipetting. ABGENE 96-WELL PCR PLATE

Applied Biosystems™ Power SYBR™ Green PCR Master Mix

Offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations. 50 ML SYBR GREEN PCR MASTER MIX, BULK PACK 50MLStore at -20 C

Applied Biosystems™ SimpliAmp™ Thermal Cycler

Small, easy-to-use, and accurate thermal cycler that suits any lab's needs for everyday PCR SIMPLIAMP THERMAL CYCLER: 3 INDEPENDENT TEMP ZONES

Taqman® TaqMan® Gene Expression Assay, SM

TAQMAN GENE EXPRESSION ASSAY250 reactions, Reporter Dye: FAM

Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit

Delivers extremely high-quality, single-stranded cDNA from 0.02 to 2μg total RNA KIT, HIGH CAPACITY CDNA RT1000 reactions

Applied Biosystems™ MicroAmp™ Optical 8-Tube Strip with Attached Optical Caps, 0.2 mL

For tube strips with domed caps for PCR experiments using 1 or 2 strips that require the use of the 96-well tray (Cat. No. 4379983) on the ProFlex™, SimpliAmp™, and Veriti™ thermal cycler 96-well blocks. X125 MicroAmp Optical 8-Tube Strip with Attached Optical Caps, 0.2 mL

Invitrogen™ Platinum™ SuperFi™ Green PCR Master Mix

2500 RXNS Platinum Superfi Green Master Mix

Applied Biosystems™ MicroAmp™ Optical 96-Well Reaction Plate

Designed to provide unmatched temperature accuracy and uniformity for fast, efficient PCR amplification X500 MICROAMP OPTICAL 96-WELL REACTION PLATE

Axygen™ 8-Strip PCR Tubes, 0.2mL

Available in a variety of colors for easy sample identification. Axygen™ 8-Strip PCR Tubes, 0.2mL are constructed of sturdy polypropylene. Each non-sterile, conical tube features thin and consistent wall thickness. Sold with flat or domed caps to prevent sample loss or without caps. X125 0,2ML THINWALL TUBE 8/STRIP

GE Healthcare Taq DNA Polymerase (cloned)

For PCR and other procedures requiring DNA polymerase activity at elevated temperatures 250 UNIT TAQ DNA POLYMERASE (CLONED) 250UNITSstore at -20°C

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

1 LT NUCLEASE-FREE WATER (NOT DEPC-TREATED) 1000MLStore at room temperature

Applied Biosystems™ Veriti™ 96-Well Thermal Cycler

The Applied Biosystems™ Veriti™ 96-Well Thermal Cycler delivers the proven reliability of Applied Biosystems™ instruments. Thermal cycler 96 well 260V 50Hz with colourtouch screen and 0.2mL block configuration

Applied Biosystems™ MicroAmp™ Optical 8-Tube Strip, 0.2mL

Maximized thermal conductivity X1000 MICROAMP OPTICAL 8-TUBE STRIP, 0.2ML 1000TUBes Store at room temperature

Applied Biosystems™ MicroAmp™ Optical 8-Cap Strips

Tight seal minimizes evaporation and contamination X300 MICROAMP OPTICAL 8-CAP STRIP STORE AT ROOMtemperature

Thermo Scientific™ RevertAid™ Premium First Strand cDNA Synthesis Kit

Perform efficient synthesis of first strand cDNA from RNA templates with this complete synthesis system. 20 RXN REVERTAID(TM) 1ST STRAND CDNA SYNTHESISkit, includes RevertAid RT, RiboLock, buffer,

Applied Biosystems™ MicroAmp™ 8-Tube Strip, 0.2mL

Tubes precisely fit industry standard well spacing 125 Strips 200UL MICROAMP 8-TUBE STRIP,0.2ML STOREat room temperature

Thermo Scientific™ 96-Well Non-Skirted Plates

96-well non-skirted plates for use in PCR and qPCR applications. Available with black lettering for improved sample tracking during pipetting. X25 THERMOFAST 96X0,2ML GREEN(VE=25Stck.)

Fisherbrand™ 0.2mL PCR Tube Strips

Ideal for use in 0.2mL, 96-well V-bottom thermal cyclers X250 Fisherbrand PCR 8-tubes 0.2ml strip w/flat cap, PP, blue - FB-1182/B

Applied Biosystems™ MicroAmp™ Fast 8-Tube Strip, 0.1mL

Maximized thermal conductivity reduces PCR amplification times X125 MICROAMP FAST 8-TUBE STRIP, 0.1ML STORE ATroom temperature

Applied Biosystems™ TaqMan™ Fast Universal PCR Master Mix (2X), No AmpErase™ UNG

TaqMan™ Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR. 1250 RXN FAST UNIVERSAL PCR MASTER MIX (2X), NO

Invitrogen™ Tris (1M), pH 7.0

Ready-to-use, certified RNase-free 500 ML 1 M TRIS, PH 7.0 500ML STORE AT ROOM TEMPERature

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