PCR Equipment and Supplies

PCR Equipment and Supplies

Applied Biosystemsâ„¢ PowerUpâ„¢ SYBRâ„¢ Green Master Mix for qPCR
Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.
Shipping Condition | Wet Ice |
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Polymerase | Dual-Lock Taq DNA Polymerase |
Format | Tube |
Product Type | Real Time PCR SYBR Master Mix |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudioâ„¢ 12k Flex, QuantStudioâ„¢ 3, QuantStudioâ„¢ 5, QuantStudioâ„¢ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudioâ„¢ 7 Flex, QuantStudioâ„¢ Absolute Q Digital PCR System, StepOneâ„¢, StepOnePlusâ„¢, ViiAâ„¢ 7 System |
Sample Type | DNA |
Reaction Speed | Fast or Standard |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Product Line | PowerUPâ„¢, SYBRâ„¢ |
Detection Method | SYBR |
Applied Biosystemsâ„¢ TaqManâ„¢ Fast Advanced Master Mix for qPCR
TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in less time on an array of qPCR instrument platforms.
Shipping Condition | Wet Ice |
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GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 2 X |
Form | Liquid |
Concentration | 2X |
For Use With (Application) | Gene Expression,miRNA analysis,Virus Detection |
PCR Method | qPCR |
Detection Method | Primer-probe |
Polymerase | AmpliTaq Fast DNA Polymerase |
Product Type | Fast Advanced Master Mix |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio Absolute Q Digital PCR System, QuantStudioâ„¢ 12k Flex, QuantStudioâ„¢ 3, QuantStudioâ„¢ 5, QuantStudioâ„¢ 6 Flex, QuantStudioâ„¢ 6 Pro, QuantStudioâ„¢ 7 Flex, QuantStudioâ„¢ 7 Pro, StepOneâ„¢, StepOnePlusâ„¢, ViiAâ„¢ 7 System |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Fast |
Product Line | TaqManâ„¢ |
Applied Biosystemsâ„¢ PowerTrackâ„¢ SYBR Green Master Mix for qPCR
PowerTrack SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows with built-in visual dye–based indicators for accurate reaction set up.
Shipping Condition | Dry Ice |
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Content And Storage | • PowerTrack SYBR Green Master Mix • 40X Yellow Sample Buffer Store at -20±5°C in the dark. |
Concentration | 2X |
For Use With (Application) | Gene Expression,Virus Detection |
PCR Method | qPCR |
Detection Method | SYBR |
Polymerase | Taq DNA Polymerase |
Format | Tube |
Product Type | Real Time PCR SYBR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Flex, QuantStudio 7 Pro, QuantStudio 12K Flex, QuantStudioâ„¢ Absolute Q Digital PCR System, Bio-Rad CFX Connectâ„¢, Bio-Rad CFX384 Touchâ„¢, Bio-Rad CFX96 Touchâ„¢, BioRad CFX96â„¢, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, ViiAâ„¢ 7 System, StepOneâ„¢ System, MJ Chromo4, MJ Opticon, StepOnePlusâ„¢ System, Roche LightCycler 480, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000 |
Sample Type | DNA |
Reaction Speed | Fast or Standard |
Product Line | Applied Biosystemsâ„¢ |
Thermo Scientificâ„¢ Maxima First Strand cDNA Synthesis Kit for RT-qPCR
The Thermo Scientific Maxima First Strand cDNA Synthesis Kit for RT-qPCR, available with or without dsDNase, is a convenient system optimized for cDNA synthesis in two-step quantitative RT-PCR (RT-qPCR) applications.
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Reverse Transcriptase | Maxima |
Final Product Type | First-Strand cDNA |
Quantity | Each |
For Use With (Application) | Real Time PCR (qPCR) |
Format | Kit |
Size (Final Product) | Up to 20 kb |
Reaction Format | Separate components |
Reaction Speed | 30 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Yes |
Optimal Reaction Temperature | 50°C to 55°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Applied Biosystemsâ„¢ TaqPathâ„¢ 1-Step RT-qPCR Master Mix, CG
TaqPath 1-Step RT-qPCR Master Mix, CG, is designed for robust and reproducible one-step pathogen detection and gene expression workflows. The single-tube 4X formulation contains thermostable MMLV reverse transcriptase, dNTPs, UNG, ROX dye, and thermostable Fast DNA polymerase.
Polymerase | Fast DNA Polymerase |
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Reverse Transcriptase | M-MLV |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudioâ„¢ 12k Flex, QuantStudioâ„¢ 3, QuantStudioâ„¢ 5, QuantStudioâ„¢ 6 Flex, QuantStudioâ„¢ 7, StepOneâ„¢, StepOnePlusâ„¢, ViiAâ„¢ 7 System, 7500 Fast Dx System, QuantStudioâ„¢ Dx |
Form | Liquid |
Sample Type | RNA, cDNA, DNA |
Reaction Speed | Fast or Standard |
Hot Start | Built-In Hot Start |
Concentration | 4X |
For Use With (Application) | Gene Expression,Virus Detection |
PCR Method | 1-step RT-qPCR |
Product Line | TaqPathâ„¢ |
Detection Method | Primer-probe |
Applied Biosystemsâ„¢ TaqManâ„¢ Fast Virus 1-Step Master Mix for qPCR
TaqMan Fast Virus 1-Step Master Mix is designed for fast, highly sensitive real-time RT-PCR, even in the presence of challenging PCR inhibitors.
Shipping Condition | Dry Ice |
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Reverse Transcriptase | M-MLV |
Form | Liquid |
For Use With (Application) | Gene Expression,Virus Detection |
PCR Method | 1-step RT-qPCR |
Detection Method | Primer-probe |
Polymerase | AmpliTaq Fast DNA Polymerase |
Format | Tube |
Product Type | 1-Step Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudioâ„¢ 12k Flex, QuantStudioâ„¢ 3, QuantStudioâ„¢ 5, QuantStudioâ„¢ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudioâ„¢ 7 Flex, QuantStudioâ„¢ Absolute Q Digital PCR System, StepOneâ„¢, StepOnePlusâ„¢, ViiAâ„¢ 7 System |
Sample Type | Total RNA, Viral DNA, Viral RNA, mRNA |
Reaction Speed | Fast or Standard |
Hot Start | Built-In Hot Start |
Product Line | TaqManâ„¢ |
Fisherbrandâ„¢ SYBRâ„¢ Green qPCR Master Mix
Fisherbrandâ„¢ SYBRâ„¢ Green qPCR Master Mix is a 2X, ready-to-use master mix designed for dye-based, quantitative amplification of DNA (cDNA and gDNA) targets by real-time PCR.
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Content And Storage | SYBR Green dye, DNA polymerase, with an antibody-mediated hot start, Heat-labile uracil-DNA glycosylase (UDG), ROX dye (passive reference dye), dNTP blend containing dUTP/dTTP, Optimized buffer components; Store at -20°C ± 10°C in the dark |
Product Type | Master Mix |
Form | Frozen |
Reaction Speed | Fast or Standard |
Label or Dye | SYBR Green,FAM |
Concentration | 2X |
For Use With (Application) | Gene Expression,DNA Quantitation,Microbial Detection,Real Time PCR (qPCR) |
PCR Method | qPCR,2-step RT-qPCR,RT-qPCR |
Detection Method | SYBR |
Applied Biosystemsâ„¢ TaqManâ„¢ Fast Advanced Master Mix for qPCR, no UNG
TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in a short time.
Shipping Condition | Wet Ice |
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GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 2 X |
Form | Liquid |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Detection Method | Primer-probe |
Polymerase | AmpliTaq Fast DNA Polymerase |
Product Type | Fast Advanced Master Mix |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudioâ„¢ 12k Flex, QuantStudioâ„¢ 3, QuantStudioâ„¢ 5, QuantStudioâ„¢ 6 Flex, QuantStudioâ„¢ 6 Pro, QuantStudioâ„¢ 7 Flex, QuantStudioâ„¢ 7 Pro, StepOneâ„¢, StepOnePlusâ„¢, ViiAâ„¢ 7 System |
Sample Type | DNA |
Reaction Speed | Fast or Standard |
Product Line | TaqManâ„¢ |
Thermo Scientificâ„¢ ABsolute QPCR Mix, low ROX
Optimized for use with hybridization probes and contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes.
Polymerase | Taq DNA Polymerase |
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GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 2 X |
Product Type | qPCR Mix |
For Use With (Equipment) | 7500 System, QuantStudioâ„¢, ViiAâ„¢ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P |
Sample Type | DNA, dsDNA |
Reaction Speed | Standard |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Product Line | ABsolute |
Detection Method | Primer-probe |
Maxima Maxima SYBR Green/ROX qPCR Master Mix (2X)
Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.
Polymerase | Taq DNA Polymerase |
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GC-Rich PCR Performance | High |
Format | Tube |
Product Type | Real Time PCR SYBR Master Mix |
For Use With (Equipment) | 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlusâ„¢ System, StepOneâ„¢ System |
Sample Type | DNA, dsDNA |
Reaction Speed | Standard |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Product Line | Maxima |
Detection Method | SYBR |
Thermo Scientificâ„¢ ABsolute QPCR Mix, ROX
Optimized for use with hybridization probes and contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes.
Polymerase | Taq DNA Polymerase |
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GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 2 X |
Product Type | qPCR Mix |
For Use With (Equipment) | 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlusâ„¢ System, StepOneâ„¢ System |
Sample Type | DNA, dsDNA |
Reaction Speed | Standard |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Product Line | ABsolute |
Detection Method | Primer-probe |
Thermo Scientificâ„¢ ABsolute QPCR Mix, SYBR Green, ROX
Optimized for SYBR Green chemistry and contains all the components necessary to perform quantitative PCR, with the exception of template and primers.
Polymerase | Taq DNA Polymerase |
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GC-Rich PCR Performance | High |
Product Type | Real Time PCR SYBR Master Mix |
For Use With (Equipment) | 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlusâ„¢ System, StepOneâ„¢ System |
Sample Type | DNA, dsDNA |
Reaction Speed | Standard |
Concentration | 2X |
Compatibility | qPCR Cyclers |
For Use With (Application) | Gene Expression,Genotyping,siRNA Validation |
PCR Method | qPCR |
Product Line | ABsolute |
Detection Method | SYBR |
Thermo Scientificâ„¢ ABsolute Blue QPCR Mix, ROX
ABsolute Blue qPCR Mixes utilize Thermo-Start Taq hot start enzyme for increased sensitivity.
Polymerase | Taq DNA Polymerase |
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GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 2 X |
Format | Tube |
Product Type | qPCR Mix |
For Use With (Equipment) | 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlusâ„¢ System, StepOneâ„¢ System |
Sample Type | DNA, dsDNA |
Reaction Speed | Standard |
Concentration | 2X |
PCR Method | qPCR |
Product Line | ABsolute Blue |
Detection Method | Primer-probe |
Thermo Scientificâ„¢ Luminaris HiGreen qPCR Master Mix
Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.
Polymerase | Taq DNA Polymerase |
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GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 2 X |
Product Type | qPCR Master Mix |
For Use With (Equipment) | BioRad CFX96â„¢, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touchâ„¢, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene |
Sample Type | DNA, dsDNA |
Reaction Speed | Standard |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Product Line | Luminaris |
Detection Method | SYBR |
Applied Biosystemsâ„¢ Path-IDâ„¢ qPCR Master Mix
Ambionâ„¢ Path-IDâ„¢ qPCR Master Mix is a convenient enzyme, buffer, and dNTP mix for TaqManâ„¢ probe-based, real-time PCR detection of DNA targets.
Shipping Condition | Dry Ice |
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Content And Storage | 2X qPCR Master Mix is stored at –20°C. Nuclease-free water may be stored at any temperature. |
Purity or Quality Grade | UP (Ultra Pure) |
Form | Liquid |
For Use With (Application) | Animal Health,Pathogen Detection |
PCR Method | qPCR |
Detection Method | Primer-probe |
Polymerase | AmpliTaq Gold DNA Polymerase |
Format | Tube |
Passive Reference Dye | ROX (Pre-mixed) |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Fast or Standard |
Hot Start | Built-In Hot Start |
Product Line | Ambionâ„¢, Path-IDâ„¢ |