PCR Supplies

A variety of products designed for use in various PCR procedures. Includes dNTPs, enzymes, assays, arrays, master mixes, reagents, and kits. Products can be used for standard, real-time, direct, high fidelity, hot start, and long range PCR protocols.

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Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR

Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

Pricing and Availability
Specifications

Shipping Condition	Wet Ice
Polymerase	Dual-Lock Taq DNA Polymerase
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 7 Flex, QuantStudio™ Absolute Q Digital PCR System, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type	DNA
Reaction Speed	Fast or Standard
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	PowerUP™, SYBR™
Detection Method	SYBR

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Fisherbrand™ SYBR™ Green qPCR Master Mix

Fisherbrand™ SYBR™ Green qPCR Master Mix is a 2X, ready-to-use master mix designed for dye-based, quantitative amplification of DNA (cDNA and gDNA) targets by real-time PCR.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Content And Storage	SYBR Green dye, DNA polymerase, with an antibody-mediated hot start, Heat-labile uracil-DNA glycosylase (UDG), ROX dye (passive reference dye), dNTP blend containing dUTP/dTTP, Optimized buffer components; Store at -20°C ± 10°C in the dark
Product Type	Master Mix
Form	Frozen
Reaction Speed	Fast or Standard
Label or Dye	SYBR Green,FAM
Concentration	2X
For Use With (Application)	Gene Expression,DNA Quantitation,Microbial Detection,Real Time PCR (qPCR)
PCR Method	qPCR,2-step RT-qPCR,RT-qPCR
Detection Method	SYBR

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Applied Biosystems™ TaqMan™ Fast Virus 1-Step Master Mix for qPCR

TaqMan Fast Virus 1-Step Master Mix is designed for fast, highly sensitive real-time RT-PCR, even in the presence of challenging PCR inhibitors.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Reverse Transcriptase	M-MLV
Form	Liquid
For Use With (Application)	Gene Expression,Virus Detection
PCR Method	1-step RT-qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq Fast DNA Polymerase
Format	Tube
Product Type	1-Step Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 7 Flex, QuantStudio™ Absolute Q Digital PCR System, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type	Total RNA, Viral DNA, Viral RNA, mRNA
Reaction Speed	Fast or Standard
Hot Start	Built-In Hot Start
Product Line	TaqMan™

Applied Biosystems™ TaqPath™ 1-Step RT-qPCR Master Mix, CG

TaqPath 1-Step RT-qPCR Master Mix, CG, is designed for robust and reproducible one-step pathogen detection and gene expression workflows. The single-tube 4X formulation contains thermostable MMLV reverse transcriptase, dNTPs, UNG, ROX dye, and thermostable Fast DNA polymerase.

Pricing and Availability
Specifications

Polymerase	Fast DNA Polymerase
Reverse Transcriptase	M-MLV
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System, 7500 Fast Dx System, QuantStudio™ Dx
Form	Liquid
Sample Type	RNA, cDNA, DNA
Reaction Speed	Fast or Standard
Hot Start	Built-In Hot Start
Concentration	4X
For Use With (Application)	Gene Expression,Virus Detection
PCR Method	1-step RT-qPCR
Product Line	TaqPath™
Detection Method	Primer-probe

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Maxima SYBR Green/ROX qPCR Master Mix (2X)

Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.

Pricing and Availability
Specifications

Polymerase	Taq DNA Polymerase
GC-Rich PCR Performance	High
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
For Use With (Equipment)	7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Sample Type	DNA, dsDNA
Reaction Speed	Standard
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	Maxima
Detection Method	SYBR

Thermo Scientific™ ABsolute QPCR Mix, SYBR Green, ROX

Optimized for SYBR Green chemistry and contains all the components necessary to perform quantitative PCR, with the exception of template and primers.

Pricing and Availability
Specifications

Polymerase	Taq DNA Polymerase
GC-Rich PCR Performance	High
Product Type	Real Time PCR SYBR Master Mix
For Use With (Equipment)	7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Sample Type	DNA, dsDNA
Reaction Speed	Standard
Concentration	2X
Compatibility	qPCR Cyclers
For Use With (Application)	Gene Expression,Genotyping,siRNA Validation
PCR Method	qPCR
Product Line	ABsolute
Detection Method	SYBR

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Applied Biosystems™ PowerTrack™ SYBR Green Master Mix for qPCR

PowerTrack SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows with built-in visual dye–based indicators for accurate reaction set up.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Content And Storage	• PowerTrack SYBR Green Master Mix • 40X Yellow Sample Buffer Store at -20±5°C in the dark.
Concentration	2X
For Use With (Application)	Gene Expression,Virus Detection
PCR Method	qPCR
Detection Method	SYBR
Polymerase	Taq DNA Polymerase
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Flex, QuantStudio 7 Pro, QuantStudio 12K Flex, QuantStudio™ Absolute Q Digital PCR System, Bio-Rad CFX Connect™, Bio-Rad CFX384 Touch™, Bio-Rad CFX96 Touch™, BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, ViiA™ 7 System, StepOne™ System, MJ Chromo4, MJ Opticon, StepOnePlus™ System, Roche LightCycler 480, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000
Sample Type	DNA
Reaction Speed	Fast or Standard
Product Line	Applied Biosystems™

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Applied Biosystems™ TaqPath™ qPCR Master Mix, CG

TaqPath qPCR Master Mix, CG, is a 2X formulation designed for gene expression and miRNA analysis. It contains thermostable Fast DNA polymerase, uracil-N-glycosylase (UNG), dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components for maximum robustness and reproducibility.

Pricing and Availability
Specifications

Content And Storage	Store at 2°C to 8°C in the dark.
Form	Liquid
Concentration	2X
For Use With (Application)	Gene Expression,Virus Detection
PCR Method	qPCR
Detection Method	Primer-probe
Polymerase	Fast DNA Polymerase
Format	Bottle
Product Type	qPCR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	QuantStudio™ Dx, 7500 Fast Dx, QuantStudio™ Absolute Q Digital PCR System
Sample Type	cDNA, DNA
Reaction Speed	Fast or Standard
Hot Start	Built-In Hot Start
Product Line	TaqPath™

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Thermo Scientific™ Maxima First Strand cDNA Synthesis Kit for RT-qPCR

The Thermo Scientific Maxima First Strand cDNA Synthesis Kit for RT-qPCR, available with or without dsDNase, is a convenient system optimized for cDNA synthesis in two-step quantitative RT-PCR (RT-qPCR) applications.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Reverse Transcriptase	Maxima
Final Product Type	First-Strand cDNA
Quantity	Each
For Use With (Application)	Real Time PCR (qPCR)
Format	Kit
Size (Final Product)	Up to 20 kb
Reaction Format	Separate components
Reaction Speed	30 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Yes
Optimal Reaction Temperature	50°C to 55°C
Technique	Reverse Transcription
Starting Material	RNA

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Applied Biosystems™ TaqMan™ Fast Advanced Master Mix for qPCR

TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in less time on an array of qPCR instrument platforms.

Pricing and Availability
Specifications

Shipping Condition	Wet Ice
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	2 X
Form	Liquid
Concentration	2X
For Use With (Application)	Gene Expression,miRNA analysis,Virus Detection
PCR Method	qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq Fast DNA Polymerase
Product Type	Fast Advanced Master Mix
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT System, QuantStudio Absolute Q Digital PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 6 Pro, QuantStudio™ 7 Flex, QuantStudio™ 7 Pro, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Fast
Product Line	TaqMan™

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Thermo Scientific™ ABsolute QPCR Mix, low ROX

Optimized for use with hybridization probes and contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes.

Pricing and Availability
Specifications

Polymerase	Taq DNA Polymerase
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	2 X
Product Type	qPCR Mix
For Use With (Equipment)	7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P
Sample Type	DNA, dsDNA
Reaction Speed	Standard
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	ABsolute
Detection Method	Primer-probe

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Applied Biosystems™ TaqMan™ Fast Advanced Master Mix for qPCR, no UNG

TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in a short time.

Pricing and Availability
Specifications

Shipping Condition	Wet Ice
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	2 X
Form	Liquid
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq Fast DNA Polymerase
Product Type	Fast Advanced Master Mix
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 6 Pro, QuantStudio™ 7 Flex, QuantStudio™ 7 Pro, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type	DNA
Reaction Speed	Fast or Standard
Product Line	TaqMan™

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Thermo Scientific™ ABsolute Blue QPCR Mix, ROX

ABsolute Blue qPCR Mixes utilize Thermo-Start Taq hot start enzyme for increased sensitivity.

Pricing and Availability
Specifications

Polymerase	Taq DNA Polymerase
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	2 X
Format	Tube
Product Type	qPCR Mix
For Use With (Equipment)	7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Sample Type	DNA, dsDNA
Reaction Speed	Standard
Concentration	2X
PCR Method	qPCR
Product Line	ABsolute Blue
Detection Method	Primer-probe

Thermo Scientific™ Luminaris Color HiGreen qPCR Master Mix

Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

Pricing and Availability
Specifications

Polymerase	Taq DNA Polymerase
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	2 X
Product Type	qPCR Master Mix
For Use With (Equipment)	BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
Sample Type	DNA, dsDNA
Reaction Speed	Standard
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	Luminaris
Detection Method	SYBR

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Thermo Scientific™ ABsolute™ qPCR Mixes

ABsolute qPCR Mixes are optimized for use with hybridization probes.

Pricing and Availability

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Applied Biosystems™ TaqPath™ qPCR Master Mix, CG Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

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Applied Biosystems™ TaqPath™ qPCR Master Mix, CG