HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.

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Thermo Scientific™ Hypersil GOLD™ C18 Selectivity HPLC Columns

Use these endcapped, ultrapure, silica-based columns to generate exceptional peak shape and resolution for HPLC and LC/MS analyses using generic gradients

Pricing and Availability

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Thermo Scientific™ Hypersil™ ODS C18 Columns

Use as a general purpose C18 phase for acidic and neutral analytes.

Pricing and Availability
Specifications

Pore Size	120 Å
Carbon Load	10%
Stationary Phase	C18
Temperature	60°C
Phase	Reversed Phase
Endcapped	Yes
Column Type	Reversed Phase
pH	2 to 8
Surface Area	170 m²/g
USP Type	L1

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Thermo Scientific™ Hypersil™ BDS C18 Columns

Use as a robust, general-purpose column for a wide range of applications in QC/QA labs.

Pricing and Availability
Specifications

Carbon Load	11%
Stationary Phase	C18
Phase	Reversed Phase
Endcapped	Yes
Column Type	Reversed Phase
Surface Area	170 m²/g
USP Type	L1

Thermo Scientific™ ProPac™ Elite WCX HPLC Column

This column provides consistent separations of therapeutic proteins including monoclonal antibodies, antibody drug conjugates, biosimilars and charge variants.

Pricing and Availability
Specifications

Pore Size	Nonporous
Stationary Phase	Carboxylate
Temperature	10°C to 60°C
Phase	Ion Exchange
Column Type	Ion Exchange
Green Features	Responsibly packaged
Max. Pressure	4500 psi (310 bar)
Column Format	Analytical Column
Particle Size	5 μm
Packing Material	Nonporous DVB Particles with Hydrophilic Coating
Mobile Phase Compatibility	Recommended: Goods Buffers and NaCl. Minimum 20 mM salt required. Do not rinse with pure DI H₂O
pH	2 to 12
Technique	Bio-LC
Product Line	ProPac

Thermo Scientific™ Hypersil™ PREP ODS C18 HPLC Columns

Perfect for routine workflows, our Hypersil PREP ODS preparative LC column is a general purpose C18 used in a broad range of USP L1 applications.

Pricing and Availability
Specifications

Pore Size	130 Å
Carbon Load	10%
Stationary Phase	C18
Temperature	60°C
Phase	Reversed Phase
Endcapped	Yes
Packing Material	Spherical, Fully Porous Silica
Column Type	Reversed Phase
pH	2 to 8
Surface Area	195 m²/g
USP Type	L1

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Thermo Scientific™ Hypercarb™ Porous Graphitic Carbon HPLC Columns

Use for enhanced retention of polar compounds and separation of structurally related analytes with stability at pH extremes and high temperature.

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Thermo Scientific™ Accucore™ aQ C18 Polar Endcapped HPLC Columns

Use this column, with its specially designed solid core, for the separation of compounds in highly aqueous mobile phases.

Pricing and Availability
Specifications

Pore Size	80 Å
Stationary Phase	C18
Temperature	70°C
Phase	Reversed Phase
Endcapped	Polar
Column Type	Reversed Phase
Surface Area	130 m²/g
Max. Pressure	14500 psi (1000 bar)
Particle Size	2.6 μm
Carbon Load	9%
Packing Material	Spherical, Solid Core, Ultrapure Silica
pH	2 to 9
Particle Shape	Spherical Solid Core
Product Line	Accucore
USP Type	L1

Thermo Scientific™ Hypersil™ BDS C8 HPLC Columns

Use as a robust, general-purpose column for QC/QA applications. They are high-quality, base-deactivated, fully endcapped, have similar C18 selectivity but slightly less retentive.

Pricing and Availability
Specifications

Pore Size	130 Å
Carbon Load	7%
Stationary Phase	C8
Temperature	60°C
Phase	Reversed Phase
Endcapped	Yes
Column Type	Reversed Phase
Surface Area	170 m²/g
USP Type	L7

Macherey-Nagel™ Nucleosil™ 100-5 C18 720120 Model EC HPLC Column

Column Format: Analytical Column; Carbon Load: 15%; Pore Size: 100 Å; Column Phase: Reversed Phase

Pricing and Availability
Specifications

Pore Size	100 Å
Stationary Phase	C18
Temperature	60°C
Phase	Reversed Phase
For Use With	HPLC
Length (Metric)	150 mm
Endcapped	Yes
Column Type	Reversed Phase
Quantity	1 Ea.
Crosslinking	Octadecyl
Surface Area	350 m²/g
Max. Pressure	5800 psi (400 bar)
Column Format	Analytical Column
Particle Size	5 μm
Type	EC Column
Carbon Load	15%
Packing Material	Silica
Mobile Phase Compatibility	Acetonitrile/water
pH	2 to 8
USP Type	L1

Thermo Scientific™ BetaBasic™ 18 HPLC Columns, 3μm Particle Size

Available in limited dimensions. Please contact Customer Service for more details.

Pricing and Availability
Specifications

Pore Size	150 Å
Particle Size	3 μm
Carbon Load	13%
Stationary Phase	C18
Phase	Reversed Phase
For Use With	LC/MS, APCI
Endcapped	Yes
Packing Material	Silica
Column Type	Reversed Phase
pH	2 to 12
Surface Area	200 m²/g
USP Type	L1
Column Format	Analytical Column

Thermo Scientific™ BioBasic™ PREP SEC 1000 Size Exclusion Chromatography HPLC Columns

Achieve outstanding size separation of water soluble compounds with BioBasic™ PREP SEC 1000 Size Exclusion Chromatography HPLC Columns.

Pricing and Availability
Specifications

Stationary Phase	Diol (OH)
Temperature	60°C
Phase	Size Exclusion
For Use With	Preparative Size Exclusion
Endcapped	No
Column Type	Size Exclusion
Surface Area	25 m²/g
Max. Pressure	4300 psi (295 bar)
Particle Size	5 μm
Carbon Load	>0.6%
Packing Material	Spherical, Fully Porous, Ultrapure Silica
Diameter (Metric)	7.8 mm
pH	2 to 8
USP Type	L59

Thermo Scientific™ Hypersil™ PREP HS C18 HPLC Columns

Maximize retention and loading with Hypersil PREP HS C18 prep LC reversed phase columns which offers high loading capacity and suitable for USP-L1 Applications.

Pricing and Availability
Specifications

Pore Size	100 Å
Carbon Load	15%
Stationary Phase	C18
Temperature	60°C
Phase	Reversed Phase
Endcapped	Yes
Packing Material	Spherical, Fully Porous, Ultrapure Silica
Column Type	Reversed Phase
pH	2 to 9
Surface Area	350 m²/g
USP Type	L1

Thermo Scientific™ Acclaim™ HILIC-10 HPLC Columns

Use this HILIC column to retain and separate highly polar molecules that are unretained by reversed-phase chromatography.

Pricing and Availability
Specifications

Pore Size	120 Å
Carbon Load	8%
Stationary Phase	Proprietary
Temperature	70°C
Phase	HILIC
For Use With	LC/MS
Endcapped	Yes
Column Type	HILIC
pH	2 to 8
Surface Area	300 m²/g
Product Line	Acclaim

Thermo Scientific™ Hypersil™ ODS C18 Column, 10μm

Provides an excellent C18 phase for a broad range of applications and a global standard for many existing methods.

Pricing and Availability
Specifications

Pore Size	120 Å
Particle Size	10 μm
Carbon Load	10%
Stationary Phase	C18
Phase	Reversed Phase
Packing Material	Silica
Column Type	Reversed Phase
Diameter (Metric)	4.6 mm
pH	2 to 8
Surface Area	170 m²/g
USP Type	L1
Column Format	Analytical Column

Thermo Scientific™ CaptureSelect™ CH1-XL MiniChrom™ Pre-packed Column

Thermo Scientific™ CaptureSelect™ CH1-XL MiniChrom™ columns are designed for bench-scale resin screening, process development, and sample preparation.

Pricing and Availability
Specifications

Shipping Condition	Approved for shipment at Room Temperature or on Wet Ice
Particle Size	65 ± 10 μm
Ligand Type	CH1-XL affinity ligand
Flow Rate	75 to 150 cm/hr. (Up to 2 bar)
Content And Storage	Store in refrigerator (2°C to 8°C).
Stationary Phase	CaptureSelect CH1-XL affinity ligand
Matrix	Agarose-based, epoxide-activated
Binding Capacity	>15 mg/mL human Fab fragment
Diameter (Metric)	0.8 cm
Product Line	CaptureSelect
Column Format	Chromatography Cartridge

HPLC Columns

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Thermo Scientific™ Hypersil GOLD™ C18 Selectivity HPLC Columns SureTRACESupports traceability with guaranteed access to certificates and proactive change notifications.Learn More

Thermo Scientific™ Hypercarb™ Porous Graphitic Carbon HPLC Columns SureTRACESupports traceability with guaranteed access to certificates and proactive change notifications.Learn More

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Thermo Scientific™ Hypersil GOLD™ C18 Selectivity HPLC Columns

Thermo Scientific™ Hypercarb™ Porous Graphitic Carbon HPLC Columns