Filtrerede søgeresultater
Søgeresultater for "IBA vector"
IBA Lifesciences StarGate pESG-IBA vektorer til pattedyrsekspression
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Designet til højniveau konstitutiv ekspression af rekombinante proteiner i en bred vifte af pattedyrværtsceller gennem CMV-promotoren med replikation i cellelinjer, der er latent inficeret med SV40 stort T-antigen.
| Mængde | 5μ g |
|---|---|
| Til brug med (udstyr) | StarGate™ System, pattedyrværtsceller |
| Format | Væske |
| Opbevaringsbuffer | 20μ L TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5μ g |
| Indhold og opbevaring | 2 °C til °8 C ved hyppig °brug, -20 C til langtidsopbevaring |
| Produkttype | Acceptor vektor for pattedyrs udtryk |
| pH | 8 |
| Holdbarhed | 12 måneder |
| Promotor | Human CMV Umiddelbart-tidligt |
| Koncentration | 250 ng/μ L |
| Inducerende middel | SV40 stort T-antigen (f.eks. COS1 eller COS7) |
| Farve | Klar |
| Til brug med (applikation) | Kloning af gener af interesse til brug ved funktionel ekspression, oprensning, påvisning, immobilisering eller adskillelse af rekombinante proteiner |
IBA Lifesciences pASK-IBA E. coli vektor
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pASK-IBA E. coli-ekspressionsvektorer, der koder for en Strep-tag ™II og chloramphenicol-resistens
| Format | Affjedring |
|---|---|
| Opbevaringsbuffer | TE buffer |
| Antibiotikaresistens bakteriel | Kloramfenicol |
| Replikationsoprindelse | F1 og ColE1 oprindelse |
| Udtrykssystem | E. coli |
| Kloningsmetode | Direkte kloning |
| Størrelsesområde | ca. 3 kb |
| Promotor | Tet |
| Koncentration | 250 ng/μ L |
| Protein tag | Strep-tag II |
IBA Lifesciences pDSG-IBA Small Mammalian Expression Vector
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Designet til proteinekspression for HEK293E-celler fra pattedyr (MEXi 293E-celler). IBA Lifesciences GmbH Small Mammalian Expression Vector er en lille transient ekspressionsvektor specielt udviklet til brug i kombination med MEXi pattedyrsekspressionssystem.
| Mængde | 5μ g |
|---|---|
| Markør Gene | Ampicillin |
| Konstitutivt eller inducerbart system | Konstitutiv |
| Udvælgelsesagent (eukaryot) | Geneticin™ (G-418) |
| Indhold og opbevaring | Anbefalet: °2 C til °8 C til hyppig brug, -°20 C til langtidsopbevaring |
| Produkttype | Vektor |
| Arter | Pattedyr |
| Kloningsmetode | Direkte kloning |
| Promotor | CMV |
| Koncentration | 250 ng/μ L |
IBA Lifesciences pCSG-IBA pattedyrekspressionsvektor uden sekretion
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Designet til episomal ekspression på højt niveau i pattedyrsværter under G418-selektion. IBA Lifesciences GmbH Mammalian Expression Vector with No Secretion er en stor ekspressionsvektor med universelle egenskaber til forbigående ekspression såvel som til generering af stabile cellelinjer (NeoR).
| Mængde | 5μ g |
|---|---|
| Markør Gene | Ampicillin |
| Konstitutivt eller inducerbart system | Konstitutiv |
| Udvælgelsesagent (eukaryot) | Geneticin™ (G-418) |
| Indhold og opbevaring | Anbefalet: °2 C til °8 C til hyppig brug, -°20 C til langtidsopbevaring |
| Produkttype | Vektor |
| Primer | Forward |
| Kloningsmetode | Direkte kloning |
| Promotor | CMV |
| Koncentration | 250 ng/μ L |
| Til brug med (applikation) | Episomal ekspression (oriP, EBNA-1) |
IBA Lifesciences pCSG-IBA pattedyrekspressionsvektor med sekretion
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Designet til episomal ekspression på højt niveau i pattedyrsværter under G418-selektion. Mammalian Expression Vector with Secretion er en stor ekspressionsvektor med universelle egenskaber til forbigående ekspression såvel som til generering af stabile cellelinjer (NeoR).
| Mængde | 5μ g |
|---|---|
| Markør Gene | Ampicillin |
| Konstitutivt eller inducerbart system | Konstitutiv |
| Udvælgelsesagent (eukaryot) | Geneticin™ (G-418) |
| Indhold og opbevaring | Anbefalet: °2 C til °8 C til hyppig brug, -°20 C til langtidsopbevaring |
| Produkttype | Vektor |
| Promotor | CMV |
| Til brug med (applikation) | Episomal ekspression (oriP, EBNA-1); BM40 sekretionssignal |
IBA Lifesciences pASG-IBA E. coli ekspressionsvektor uden sekretion
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Reguler ekspressionen af proteiner med en vektor, der bærer den inducerbare tetracyclinpromotor/operator. IBA Lifesciences GmbH E. coli-ekspressionsvektor uden sekretion muliggør ekspression af proteiner i E.coli.
| Mængde | 5μ g |
|---|---|
| Markør Gene | Ampicillin |
| Indhold og opbevaring | Anbefalet: °2 C til °8 C til hyppig brug, -°20 C til langtidsopbevaring |
| Produkttype | Vektor |
| Promotor | Tet |
| Koncentration | 250 ng/μ L |
| Inducerende middel | Inducerbar med anhydrotetracyclin |
IBA Lifesciences pASG-IBA E. coli ekspressionsvektor med sekretion
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Reguler ekspressionen af proteiner med en vektor, der bærer den inducerbare tetracyclinpromotor/operator. E. coli-ekspressionsvektor med sekretion muliggør ekspression af proteiner i E. coli.
| Mængde | 5μ g |
|---|---|
| Markør Gene | Ampicillin |
| Indhold og opbevaring | Anbefalet: °2 C til °8 C til hyppig brug, -°20 C til langtidsopbevaring |
| Produkttype | Vektor |
| Promotor | Tet |
| Inducerende middel | Inducerbar med anhydrotetracyclin |
| Til brug med (applikation) | ompA for periplasmatisk sekretion |
IBA Lifesciences pENTRY-IBA51 vektor
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IBA Lifesciences GmbH Entry Vector pENTRY-IBA51 er påkrævet for at generere donor-vektorer til efterfølgende Destination Vector-generering.
5 UG E.coli vectors with tet promoter for periplas
12866486 5 UG E.coli vectors with tet promoter for periplas
IBA Lifesciences StarGate pPSG-IBA Acceptor Vectors for E. coli Expression
Achieves high-level expression in E. coli by the T7 promoter by providing specified genetic surroundings for optimal expression and purification of non-toxic proteins.
| Mængde | 5 μg |
|---|---|
| Til brug med (udstyr) | StarGate™ System, E. Coli BL21(DE3) |
| Format | Liquid |
| Opbevaringsbuffer | 20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg |
| Indhold og opbevaring | 2°C to 8°C for frequent usage, -20°C for long-term storage |
| pH | 8 |
| Holdbarhed | 12 months |
| Promotor | T7 |
| Koncentration | 250 ng/μL |
| Inducerende middel | E. coli host with T7 RNA polymerase gene (e.g. BL21(DE3)) |
| Farve | Clear |
| Til brug med (applikation) | Cloning genes of interest for use in functional expression, purification, detection, immobilization or separation of recombinant proteins |
IBA Lifesciences StarGate pLSG-IBA Vectors for Baculo
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Transfer vectors that allow for high-level expression in insect cells by introducing the gene of interest into the Polyhedrin gene locus of AcMNPV DNA by homologous recombination.
| Mængde | 5μ g |
|---|---|
| Til brug med (udstyr) | StarGate™ System, Insect Host Cells |
| Format | Væske |
| Opbevaringsbuffer | 20μ L TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5μ g |
| Indhold og opbevaring | 2 °C til °8 C ved hyppig °brug, -20 C til langtidsopbevaring |
| Produkttype | Acceptor Vector for bacula (insect cell) expression |
| pH | 8 |
| Holdbarhed | 12 måneder |
| Promotor | Polyhedrin |
| Koncentration | 250 ng/μ L |
| Inducerende middel | BacPAK6 linearized A cmNPV DNA (Clontech) or circular flashBAC modified A cmNPV DNA (Oxford Expression Technologies) |
| Farve | Klar |
| Til brug med (applikation) | Kloning af gener af interesse til brug ved funktionel ekspression, oprensning, påvisning, immobilisering eller adskillelse af rekombinante proteiner |
IBA Lifesciences StarGate pYSG-IBA Vectors for Yeast Expression
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Designed for high-level expression of recombinant proteins in Yeast by introducing the gene of interest under the control of the Cu++-inducible CUP1 promoter.
| Til brug med (udstyr) | StarGate™ System, Yeast Host Cells |
|---|---|
| Opbevaringsbuffer | 20μ L TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5μ g |
| Markør Gene | leu2-d and URA3 |
| Promotor | CUP1 |
| Koncentration | 250 ng/μ L |
| Farve | Klar |
| Mængde | 5μ g |
| Format | Væske |
| Indhold og opbevaring | 2 °C til °8 C ved hyppig °brug, -20 C til langtidsopbevaring |
| Produkttype | Acceptor Vector for yeast expression |
| pH | 8 |
| Holdbarhed | 12 måneder |
| Inducerende middel | Copper sulfate |
| Til brug med (applikation) | Kloning af gener af interesse til brug ved funktionel ekspression, oprensning, påvisning, immobilisering eller adskillelse af rekombinante proteiner |
IBA Lifesciences StarGate pESG-IBA vektorer til pattedyrsekspression
Designet til højniveau konstitutiv ekspression af rekombinante proteiner i en bred vifte af pattedyrværtsceller gennem CMV-promotoren med replikation i cellelinjer, der er latent inficeret med SV40 stort T-antigen.
IBA Solutions For Life Science StarGate™ Upstream Fusion Vector pNFUSE-IBA
For upstream positioning of an inserted gene in the final Fusion Donor vector when linking two or more genes cloned in separate Donor Vectors using the StarGate™ transfer cloning system.
| Mængde | 5 μg |
|---|---|
| Til brug med (udstyr) | Stargate™ system derivatives |
| Format | Solution, liquid |
| Opbevaringsbuffer | 20μL TE buffer: 10mM Tris-HCl, 1mM EDTA per 5μL pCFUSE-IBA11 |
| Oversigt | Resistance: ampicillin |
| Indhold og opbevaring | 2°C to 8°C for frequent usage, -20°C for long-term storage |
| Produkttype | Fusion Vector |
| pH | 8 |
| Holdbarhed | 12 months |
| Koncentration | 250 ng/μL |
| Farve | Clear |
| Til brug med (applikation) | Upstream positioning, fusing cloned genes of interest |
IBA Lifesciences pCSG-IBA Mammalian Expression Vector with No Secretion
Designed for high-level episomal expression in mammalian hosts under G418 selection. IBA Lifesciences GmbH Mammalian Expression Vector with No Secretion is a large expression vector with universal features for transient expression as well as for generation of stable cell lines (NeoR).