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IBA Lifesciences StarGate pYSG-IBA Vectors for Yeast Expression
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Vektor:
pYSG-IBA 103
pYSG-IBA 105
pYSG-IBA 123
pYSG-IBA 143
pYSG-IBA 145
pYSG-IBA 162
pYSG-IBA 164
pYSG-IBA 167
pYSG-IBA 168
pYSG-IBA 23
pYSG-IBA 25
pYSG-IBA 3
pYSG-IBA 33
pYSG-IBA 43
pYSG-IBA 45
pYSG-IBA 5
pYSG-IBA 62
pYSG-IBA 63
pYSG-IBA 64
pYSG-IBA 65
pYSG-IBA wt1
Pakningsstørrelse:
5µg
Beskrivelse
pYSG-IBA yeast expression vectors
The pYSG-IBA vector allows the high-level expression of proteins in yeast.
- The vector carries the copper inducible promoter (CUP1) for controlled high-level expression.
- The expressed recombinant protein will be localized in the cytoplasm.
- High-level expression in yeast with high copy number
- Favors correct protein folding and the production of soluble proteins - inclusion bodies rarely form
- Tightly regulated expression due to Cu++-inducible CUP1 promoter
- Ampicillin resistance gene and markers Leu2-d and URA3 in every vector
- Leu2-d auxotrophy marker for selection to increase plasmid copy number during expression
- URA3 auxotrophy marker for selection after transformation
Please note that cloning into pYSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pYSG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.
Tekniske data
Tekniske data
| Format | Væske |
| Til brug med (udstyr) | StarGate™ System, Yeast Host Cells |
| Inducerende middel | Copper sulfate |
| Markør Gene | leu2-d and URA3 |
| pH | 8 |
| Promotor | CUP1 |
| Produkttype | Acceptor Vector for yeast expression |
| Opbevaringsbuffer | 20μ L TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5μ g |
| Indhold og opbevaring | 2 °C til °8 C ved hyppig °brug, -20 C til langtidsopbevaring |
| Protein tag | Umærket |
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