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  5. IBA Lifesciences StarGate pPSG-IBA Acceptor Vectors for E. coli Expression

IBA Lifesciences StarGate pPSG-IBA Acceptor Vectors for E. coli Expression

Artikelnummer. 15683758 Shop alle IBA Lifesciences produkter
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Vektor:
pPSG-IBA 105
pPSG-IBA 123
pPSG-IBA 164
pPSG-IBA 168
pPSG-IBA 23
pPSG-IBA 25
pPSG-IBA 3
pPSG-IBA 33
pPSG-IBA 5
pPSG-IBA 62
pPSG-IBA 63
pPSG-IBA 64
pPSG-IBA 65
pPSG-IBA wt1
pPSG-IBA103
pPSG-IBA143
pPSG-IBA145
pPSG-IBA162
pPSG-IBA167
pPSG-IBA35
pPSG-IBA43
pPSG-IBA45
Pakningsstørrelse:
5µg
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Artikelnummer. 15683758

Brand: IBA Lifesciences 54233001

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Beskrivelse

Achieves high-level expression in E. coli by the T7 promoter by providing specified genetic surroundings for optimal expression and purification of non-toxic proteins.

pPSG-IGA E. coli expression vectors:

pPSG-IBA vectors use the T7 promoter and T7 RNA polymerase for high-level transcription of the gene of interest.

  • As the T7 promoter is stronger than the tet promoter, pPSG-IBA vectors are recommended in cases where expression with the tet promoter does not lead to significant yields of the recombinant protein.

    • High-level expression in E. coli by bacteriophage T7 promoter
    • High-level transcription by T7 RNA polymerase in BL21 strains
    • High-level expression of non-toxic proteins
    • Induction by IPTG
    • Suitable for in vitro transcription/translation

    StarGate System

    IBA developed StarGate to provide a simple, fast and highly efficient cloning process for genes of interest (GOI) for use in functional expression, purification, detection, immobilization or separation of recombinant proteins:

    • Minimal extra modification of the gene due to short combinatorial sites
    • Inherent high-level cloning efficiency due to a directed reaction (no equilibrium)
    • GOIs are cloned to obtain a Donor Vector; 2 or more genes can be combined using Fusion Cloning
    • Genes are then transferred into Acceptor Vectors that provide optimal genetic surroundings
    • The final expression vector, the Destination Vector, is then placed into the respective host

    • Tekniske data

    Format Liquid
    Til brug med (udstyr) StarGate™ System, E. Coli BL21(DE3)
    Inducerende middel E. coli host with T7 RNA polymerase gene (e.g. BL21(DE3))
    pH 8
    Promotor T7
    Produkttype Acceptor Vector for E. coli expression
    Opbevaringsbuffer 20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg
    Indhold og opbevaring 2°C to 8°C for frequent usage, -20°C for long-term storage
    Protein tag His Tag for C-terminal
    Koncentration 250 ng/μL
    Mængde 5 μg
    Vektor pPSG-IBA 33
    Farve Clear
    Til brug med (applikation) Cloning genes of interest for use in functional expression, purification, detection, immobilization or separation of recombinant proteins
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