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IBA Lifesciences pDSG-IBA Small Mammalian Expression Vector

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Mængde:
5 μg
Pakningsstørrelse:
5µg
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Artikelnummer. 15683878

Brand: IBA Lifesciences 55209001

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Beskrivelse

Designed for protein expression for mammalian HEK293E cells (MEXi 293E cells). IBA Lifesciences GmbH Small Mammalian Expression Vector is a small transient expression vector especially developed for the use in combination with the MEXi mammalian expression system.

pDSG-IBA vectors for MEXi mammalian expression system

  • Small transient expression vector especially developed for the use in combination with the MEXi mammalian expression system.
  • Designed for high-level stable and non-replicative transient expression in most mammalian hosts containing the following elements:
    • Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells
    • Episomal replication through Epstein Barr Virus replication origin (oriP) requires chromosomally expressed nuclear antigen encoded by EBNA-1
    • Ampicillin resistance and ColE1 replication origin (pUC) for propagation in E. coliin a high plasmid copy number

Please note that cloning into pDSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pDSG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.

Tekniske data

Konstitutivt eller inducerbart system Constitutive
Markør Gene Ampicillin
Promotor CMV
Produkttype Vector
Udvælgelsesagent (eukaryot) Geneticin™ (G-418)
Indhold og opbevaring Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage
Protein tag His Tag (6x)
Primer Forward
Mængde 5 μg
Vektor pDSG-IBA33
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