Enzymes and Inhibitors

Thermo Scientific™ I-SceI

Cut at TAGGGATAA^CAGGGTAAT sites with I-SceI homing enzyme, which performs best at 37°C in Tango buffer. I-SCEI 10U/UL 250U

Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA)

Optimize electrophoresis of both genomic and large supercoiled DNA in agarose and polyacrylamide gels with Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA). 1 LT 50X TAE BUFFER (TRIS-ACETATE-EDTA), 1X CONCENtration includes 40 mM Tris, 20 mM acetic acid, 1

Thermo Scientific™ Bovine Serum Albumin (BSA)

Stabilize enzymes, increase PCR yields, and prevent adhesion of enzymes with Thermo Scientific™ Bovine Serum Albumin. 250 UL BOVINE SERUM ALBUMIN (BSA), 20MG/ML, 5MG(0.25mL), supplied in 10 mM Tris-HCl (pH7.4 at

Thermo Scientific™ FastDigest™ NheI: PROMO

Thermo Scientific FastDigest NheI restriction enzyme recognizes G^CTAGC site and cuts best at 37°C in 5 to 15 minutes using universal FastDigest Buffer. Isoschizomers: AsuNHI, BmtI. FASTDIGEST NHEI 50UL

Thermo Scientific™ FastDigest BsiWI (Pfl23II)

Cut at C^GTACG sites with Thermo Scientific™ FastDigest™ BsiWI (Pfl23II), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: PspLI). Restriction enzyme, FastDigest Pfl23II, 50µL

Thermo Scientific™ FastDigest KpnI

Perform single-, double- or multiple DNA digestion within 5-15 minutes withthis advanced line of restriction enzymes, 100% active in one universal buffer. FASTDIGEST KPNI 300UL

Thermo Scientific™ BsuRI (HaeIII)

Cut at GG^CC sites with BsuRI (HaeIII) restriction enzyme, which performs best at 37°C in R buffer (Isoschizomers: BshFI, BsnI, BspANI, HaeIII, PhoI). RESTRICTION ENZYME, CONVENTIONAL, BSURI (HAEIII),10u/µL cuts GG¬CC at 37°C (supplied with 1mL of

Thermo Scientific™ FastDigest BbsI (BpiI)

Cut at GAAGAC(2/6)^ sites with Thermo Scientific™ FastDigest™ BbsI (BpiI), which performs best using universal FastDigest Buffer (Isoschizomers: BpuAI, BstV2I). Restriction enzyme, FastDigest BpiI, 20µL

Thermo Scientific™ FastDigest MspI

Perform single-, double- or multiple DNA digestion within 5-15 minutes withthis advanced line of restriction enzymes, 100% active in one universal buffer. 400 UL RESTRICTION ENZYME, FASTDIGEST, MSPI, CUTSC¬CGG, 1µL cleaves 1µg DNA in 5 minutes at 37°C

Thermo Scientific™ FastDigest BsmBI (Esp3I)

Cut at CGTCTC(1/5)^ sites with Thermo Scientific™ FastDigest™ BsmBI (Esp3I), which peforms best at 37°C in 5-15 minutes using universal FastDigest Buffer. Restriction enzyme, FastDigest Esp3I, 20µL

Thermo Scientific™ FastDigest BfaI (FspBI)

Cut at C^TAG sites with Thermo Scientific™ FastDigest™ BfaI (FspBI), which performs best using universal FastDigest Buffer (Isoschizomers: BfaI, MaeI, XspI). Restriction enzyme, FastDigest FspBI, 50µL

Thermo Scientific™ FastDigest NsiI (Mph1103I)

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. Restriction enzyme, FastDigest Mph1103I, 100µL

Thermo Scientific™ Psp1406I (AclI)

The Psp1406I (AclI) restriction enzyme recognizes AA^CGTT sites and cuts best at 37°C in Tango buffer (Isoschizomers: AclI). PSP1406I (ACLI) 10U/UL 1500U

Thermo Scientific™ FastDigest™ EcoRI

The FastDigest EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer. X5 FASTDIGEST ECORI 500UL

Invitrogen™ Anza™ 5 BamHI

Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System. Anza 5 BamHI (24,000 units)

Thermo Scientific™ Esp3I (BsmBI)

The Esp3I (BsmBI) restriction enzyme recognizes CGTCTC(1/5)^ sites and cuts best at 37°C in Tango (+DTT) buffer (Isoschizomers: BsmBI). ESP3I (BSMBI) 10U/UL 1000U

Thermo Scientific™ MvaI (BstNI)

Cut at CC^WGG sites with MvaI (BstNI) restriction enzyme, which performs best at 37°C in R buffer (Isoschizomers: AjnI, BseBI, Bst2UI, BstNI, BstOI, Psp6I, PspGI). 2000 UNIT RESTRICTION ENZYME, CONVENTIONAL, MVAI(BstNI), 10u/uL, cuts CC¬WGG at 37°C (supplied

Thermo Scientific™ FastDigest™ NotI

The FastDigest NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: CciNI). 250 UL RESTRICTION ENZYME, FASTDIGEST, NOTI, CUTSGC¬GGCCGC, 1µL cleaves 1µg DNA in 5 minutes at

Thermo Scientific™ FastDigest™ HindIII

The FastDigest HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer. X5 FASTDIGEST HINDIII 500UL

Thermo Scientific™ FastDigest™ BamHI

Cut at G^GATCC sites with Thermo Scientific™ FastDigest™ BamHI restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer. X5 FASTDIGEST BAMHI 500UL

Thermo Scientific™ FastDigest AatII

Cut at GACGT^C sites with Thermo Scientific™ FastDigest™ AatII, which performs best at 37°C in 5-15 minutes using universal FastDigest™ Buffer (Isoschizomers: ZraI). 50 UL RESTRICTION ENZYME, FASTDIGEST, AATII, CUTSGACGT¬C, 1µL cleaves 1µg DNA in 15 minutes at

Thermo Scientific™ FastDigest HaeIII (BsuRI)

Perform single-, double- or multiple DNA digestion within 5-15 minutes withthis advanced line of restriction enzymes, 100% active in one universal buffer. Restriction enzyme, FastDigest BsuRI, 400µL

Thermo Scientific™ FastDigest™ Value Pack

FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in a single buffer. FASTDIGEST VALUE PACK

Thermo Scientific™ FastDigest™ XbaI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. 750 UL RESTRICTION ENZYME, FASTDIGEST, XBAI, CUTST¬CTAGA, 1µL cleaves 1µg DNA in 5 minutes at 37°C

Thermo Scientific™ 10X Reaction Buffer with MgCl2 for DNase I

Optimize reaction condions for RNase-free DNase I with this 10X reaction Tris-HCl (pH 7.5) buffer containing MgCl2. 10X DNASE I RNASE-FREE 1ML

OPTIZYME™ XbaI, Fisher BioReagents™

Optizyme Xba I restriction enzyme

Thermo Scientific™ BplI

Cut at ^(8/13)GAG(N)5CTC(13/8)^ sites with BplI restriction enzyme, which performs best at 37°C in Tango(+SAM) buffer. BPLI 5U/UL 500U

Thermo Scientific™ Bsh1236I (BstUI)

Cut at CG^CG sites with Bsh1236I (BstUI) restriction enzyme, which performs best at 37°C in R buffer (Isoschizomers: AccII, BspFNI, BstFNI, BstUI, MvnI). BSH1236I (BSTUI) 10U/UL 500U

Thermo Scientific™ Bpu1102I (BlpI)

Cut at GC^TNAGC sites with Bpu1102I (BlpI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: BlpI, Bsp1720I, CelII). BPU1102I (BLPI) 10U/UL 1000U

Thermo Scientific™ FastDigest DraIII (AdeI)

Cut at CACNNN^GTG sites with Thermo Scientific™ FastDigest™ DraIII (AdeI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer. Restriction enzyme, FastDigest AdeI, 50µL

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