Enzymes and Inhibitors

Thermo Scientific™ I-SceI

Cut at TAGGGATAA^CAGGGTAAT sites with I-SceI homing enzyme, which performs best at 37°C in Tango buffer. I-SCEI 10U/UL 250U

Thermo Scientific™ FastDigest AvrII (XmaJI)

Cut at C^CTAGG sites with Thermo Scientific™ FastDigest™ AvrII (XmaJI), which performs best using universal FastDigest Buffer (Isoschizomers: AspA2I, BlnI). Restriction enzyme, FastDigest XmaJI, 20µL

Thermo Scientific™ SmaI

Cut at CCC^GGG sites with SmaI restriction enzyme, which performs best at 30°C in Tango buffer (Isoschizomers: TspMI, XmaCI, XmaI). X5 SMAI 10U/UL 1200U

Thermo Scientific™ FastDigest AgeI (BshTI)

Cut at A^CCGGT sites with Thermo Scientific™ FastDigest™ AgeI (BshTI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer. Restriction enzyme, FastDigest BshTI, 20µL

Thermo Scientific™ FastDigest Bsp119I

Cut at TT^CGAA sites with Thermo Scientific™ FastDigest™ Bsp119I, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer. 200 UL RESTRICTION ENZYME, FASTDIGEST, BSP119I,cuts TT¬CGAA, 1µL cleaves 1µg DNA in 5 minutes at

Thermo Scientific™ FastDigest BfaI (FspBI)

Cut at C^TAG sites with Thermo Scientific™ FastDigest™ BfaI (FspBI), which performs best using universal FastDigest Buffer (Isoschizomers: BfaI, MaeI, XspI). Restriction enzyme, FastDigest FspBI, 50µL

Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA)

Optimize electrophoresis of both genomic and large supercoiled DNA in agarose and polyacrylamide gels with Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA). 1 LT 50X TAE BUFFER (TRIS-ACETATE-EDTA), 1X CONCENtration includes 40 mM Tris, 20 mM acetic acid, 1

Thermo Scientific™ FastDigest AatII

Cut at GACGT^C sites with Thermo Scientific™ FastDigest™ AatII, which performs best at 37°C in 5-15 minutes using universal FastDigest™ Buffer (Isoschizomers: ZraI). 50 UL RESTRICTION ENZYME, FASTDIGEST, AATII, CUTSGACGT¬C, 1µL cleaves 1µg DNA in 15 minutes at

Thermo Scientific™ FastDigest AluI

Cut at AG^CT sites with Thermo Scientific™ FastDigest™ AluI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: AluBI). 100 UL RESTRICTION ENZYME, FASTDIGEST, ALUI, CUTSAG¬CT, 1µL cleaves 1µg DNA in 15 minutes at 37°C

Thermo Scientific™ PaeI (SphI)

The PaeI (SphI) restriction enzyme recognizes GCATG^C sites and cuts best at 37°C in B buffer (Isoschizomers: BbuI, SphI). PAEI (SPHI) 10U/UL 2500U

Thermo Scientific™ FastDigest™ NotI

The FastDigest NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: CciNI). 20 UL RESTRICTION ENZYME, FASTDIGEST, NOTI, CUTSGC¬GGCCGC, 1µL cleaves 1µg DNA in 5 minutes at

Thermo Scientific™ ATP

Optimize many molecular biology applications using this ATP, a highly stable nucleotide with 99% purity. ATP 100MM 0.25ML

Thermo Scientific™ FastDigest NciI (BcnI)

Perform single-, double- or multiple DNA digestion within 5-15 minutes withthis advanced line of restriction enzymes, 100% active in one universal buffer. Restriction enzyme, FastDigest BcnI, 200µL

Thermo Scientific™ Bovine Serum Albumin (BSA)

Stabilize enzymes, increase PCR yields, and prevent adhesion of enzymes with Thermo Scientific™ Bovine Serum Albumin. 250 UL BOVINE SERUM ALBUMIN (BSA), 20MG/ML, 5MG(0.25mL), supplied in 10 mM Tris-HCl (pH7.4 at

Thermo Scientific™ FastDigest NsiI (Mph1103I)

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. Restriction enzyme, FastDigest Mph1103I, 100µL

Thermo Scientific™ FastDigest BsiWI (Pfl23II)

Cut at C^GTACG sites with Thermo Scientific™ FastDigest™ BsiWI (Pfl23II), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: PspLI). Restriction enzyme, FastDigest Pfl23II, 50µL

Thermo Scientific™ BsuRI (HaeIII)

Cut at GG^CC sites with BsuRI (HaeIII) restriction enzyme, which performs best at 37°C in R buffer (Isoschizomers: BshFI, BsnI, BspANI, HaeIII, PhoI). RESTRICTION ENZYME, CONVENTIONAL, BSURI (HAEIII),10u/µL cuts GG¬CC at 37°C (supplied with 1mL of

Thermo Scientific™ DpnI

Cut at Gm6A^TC sites with DpnI restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: MalI). DPNI 10U/UL 2500U

Invitrogen™ Anza™ 10 DpnI

Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System. Anza 10 DpnI (800 units)

Thermo Scientific™ AarI

Cut at CACCTGC(4/8)^ sites with AarI restriction enzyme, which performs best at 37°C in its own unique (+oligo) buffer. 25 UNIT RESTRICTION ENZYME, CONVENTIONAL, AARI,2u/ul, 25 u, cuts CACCTGC(4/8)¬ at 37°C (supplied

Thermo Scientific™ FastDigest™ BamHI

Cut at G^GATCC sites with Thermo Scientific™ FastDigest™ BamHI restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer. FASTDIGEST BAMHI 800UL

OPTIZYME™ AluI, Fisher BioReagents™

Optizyme Alu I restriction enzyme

Thermo Scientific™ PscI (PciI)

The PscI (PciI) restriction enzyme recognizes A^CATGT sites and cuts best at 37°C in Tango buffer (Isoschizomers: PciI). PSCI (PCII) 10U/UL 200U

Thermo Scientific™ Eco57I (AcuI)

The Eco57I (AcuI) restriction enzyme recognizes CTGAAG(16/14)^ sites and cuts best at 37°C in G (+SAM) buffer (Isoschizomers: AcuI). ECO57I (ACUI) 5U/UL 200U

Thermo Scientific™ SfiI

Cut at GGCCNNNN^NGGCC sites with SfiI restriction enzyme, which performs best at 50°C in G buffer. SFII 10U/UL 1000U

Thermo Scientific™ FastDigest TatI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. 20 UL RESTRICTION ENZYME, FASTDIGEST, TATI, CUTSW¬GTACW, 1µL cleaves 1µg DNA in 15 minutes at

Thermo Scientific™ Csp6I (CviQI)

Cut at G^TAC sites with Csp6I (CviQI) restriction enzyme, which performs best at 37°C in B buffer (Isoschizomers: AfaI, CviQI, RsaNI). 1500 UNIT RESTRICTION ENZYME, CONVENTIONAL, CSP6I(CviQI), 10u/uL, cuts G¬TAC at 37°C (supplied

Thermo Scientific™ Buffer BseXI

Optimize digestions with BseXI restriction enzyme using this buffer, premixed with BSA for enhanced stability. 10X BUFFER BSEXI 1ML

Thermo Scientific™ FastDigest AflII (BspTI)

Cut at C^TTAAG sites with Thermo Scientific™ FastDigest™ AflII (BspTI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer. Restriction enzyme, FastDigest BspTI, 50µL

Thermo Scientific™ CpoI (RsrII)

The CpoI (RsrII) restriction enzyme recognizes CG^GWCCG sites and cuts best at 37°C in Tango buffer (Isoschizomers: CspI, Rsr2I, RsrII). CPOI (RSRII) 10U/UL 200U

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