Enzymes and Inhibitors

Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA)

Optimize electrophoresis of both genomic and large supercoiled DNA in agarose and polyacrylamide gels with Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA). 1 LT 50X TAE BUFFER (TRIS-ACETATE-EDTA), 1X CONCENtration includes 40 mM Tris, 20 mM acetic acid, 1

Thermo Scientific™ FastDigest SapI (LguI)

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. Restriction enzyme, FastDigest LguI, 20µL

Thermo Scientific™ PauI (BssHII)

The PauI (BssHII) restriction enzyme recognizes G^CGCGC sites and cuts best at 37°C in R buffer (Isoschizomers: BsePI, BssHII). PAUI (BSSHII) 10U/UL 1000U

Thermo Scientific™ FastDigest AflII (BspTI)

Cut at C^TTAAG sites with Thermo Scientific™ FastDigest™ AflII (BspTI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer. Restriction enzyme, FastDigest BspTI, 50µL

Thermo Scientific™ AdeI (DraIII)

Cut at CACNNN^GTG sites with AdeI (DraIII) restriction enzyme, which performs best at 37°C in G buffer (Isoschizomers: DraIII). RESTRICTION ENZYME, CONVENTIONAL, ADEI (DRAIII),10u/ul, cuts CACNNN¬GTG at 37°C (supplied with

Thermo Scientific™ FastDigest SwaI (SmiI)

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. Restriction enzyme, FastDigest SmiI, 200µL

Thermo Scientific™ FastDigest™ NotI

The FastDigest NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: CciNI). 250 UL RESTRICTION ENZYME, FASTDIGEST, NOTI, CUTSGC¬GGCCGC, 1µL cleaves 1µg DNA in 5 minutes at

Thermo Scientific™ Alw26I (BsmAI)

Cut at GTCTC(1/5)^ sites with Alw26I (BsmAI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: BsmAI, BstMAI). RESTRICTION ENZYME, CONVENTIONAL, ALW26I, 10U/µLcuts GTCTC(1/5)¬ at 37°C (supplied with 1mL of

Thermo Scientific™ FastDigest PvuI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. FASTDIGEST PVUI 20UL

Thermo Scientific™ 10X T4 DNA Ligase Buffer

Safely ligate samples with this buffer, for use with T4 DNA Ligase and tested for the absence of endo- and exodeoxyribonucleases and ribonucleases. X10 T4 DNA LIGASE 1.5ML

Thermo Scientific™ FastDigest BspHI (PagI)

Cut at T^CATGA sites with Thermo Scientific™ FastDigest™ BspHI (PagI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: CciI, RcaI). Restriction enzyme, FastDigest PagI, 50µL

Thermo Scientific™ FastDigest™ NheI: PROMO

Thermo Scientific FastDigest NheI restriction enzyme recognizes G^CTAGC site and cuts best at 37°C in 5 to 15 minutes using universal FastDigest Buffer. Isoschizomers: AsuNHI, BmtI. FASTDIGEST NHEI 50UL

Thermo Scientific™ FastDigest BbsI (BpiI)

Cut at GAAGAC(2/6)^ sites with Thermo Scientific™ FastDigest™ BbsI (BpiI), which performs best using universal FastDigest Buffer (Isoschizomers: BpuAI, BstV2I). Restriction enzyme, FastDigest BpiI, 20µL

Thermo Scientific™ SgsI (AscI)

The SgsI (AscI) restriction enzyme recognizes GG^CGCGCC sites and cuts best at 37°C in Tango buffer (Isoschizomers: AscI, PalAI). 1500 UNIT RESTRICTION ENZYME, CONVENTIONAL, SGSI(AscI), 5u/ul, cuts GG¬CGCGCC at 37°C (supplied

Thermo Scientific™ FastDigest NsiI (Mph1103I)

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. Restriction enzyme, FastDigest Mph1103I, 100µL

Thermo Scientific™ FastDigest PvuII

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. 400 UL RESTRICTION ENZYME, FASTDIGEST, PVUII,cuts CAG¬CTG, 1µL cleaves 1µg DNA in 5 minutes at

Thermo Scientific™ I-SceI

Cut at TAGGGATAA^CAGGGTAAT sites with I-SceI homing enzyme, which performs best at 37°C in Tango buffer. I-SCEI 10U/UL 250U

Thermo Scientific™ FastDigest SphI (PaeI)

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. Restriction enzyme, FastDigest PaeI, 20µL

Thermo Scientific™ FastDigest SnaBI (Eco105I)

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. Restriction enzyme, FastDigest Eco105I, 50µL

Thermo Scientific™ FastDigest™ Eco32I

Cut at GAT^ATC sites with Thermo Scientific™ FastDigest™ EcoRV (Eco32I), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: EcoRV). Restriction enzyme, FastDigest Eco32I, 400µL

Thermo Scientific™ FastDigest™ NcoI

The FastDigest NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Bsp19I). 300 UL RESTRICTION ENZYME, FASTDIGEST, NCOI, CUTSC¬CATGG, 1µL cleaves 1µg DNA in 5 minutes at 37°C

Thermo Scientific™ BplI

Cut at ^(8/13)GAG(N)5CTC(13/8)^ sites with BplI restriction enzyme, which performs best at 37°C in Tango(+SAM) buffer. BPLI 5U/UL 500U

Invitrogen™ Anza™ 76 VspI

Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System. 1500UNIT ANZA 76 VSPI

Thermo Scientific™ SgeI

Cut at m5CNNG(9/13)^ sites with SgeI restriction enzyme, which performs best at 37°C in its own unique buffer. RESTRICTION ENZYME, CONVENTIONAL, SGEI, 3U/UL,cuts m5CNNG(9/13)¬ at 37°C (supplied with 1mL of

Thermo Scientific™ Buffer G

Ensure the optimum reaction conditions for restriction enzymes with Thermo Scientific™ 10X Buffer G, premixed with BSA for enhanced stability. X10 BUFFER G 5X1ML

Invitrogen™ Anza™ 99 XagI

Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System. 800UNIT ANZA 99 XAGI

Thermo Scientific™ HphI

The HphI restriction enzyme recognizes GGTGA(8/7)^ sites and cuts best at 37°C in B buffer (Isoschizomers: AsuHPI). 300 UNIT RESTRICTION ENZYME, CONVENTIONAL, HPHI,10u/ul, cuts GGTGA(8/7)¬ at 37°C (supplied with

Thermo Scientific™ SatI (Fnu4HI)

The SatI (Fnu4HI) restriction enzyme recognizes GC^NGC sites and cuts best at 37°C in G buffer (Isoschizomers: Fnu4HI, Fsp4HI, ItaI). SATI (FNU4HI) 10U/UL 200U

Thermo Scientific™ EcoO109I (DraII)

Cut at RG^GNCCY sites with EcoO109I (DraII) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: DraII). RESTRICTION ENZYME, CONVENTIONAL, ECOO109I (DRAII), 10u/uL, cuts RG¬GNCCYat 37°C (supplied with 1mL

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