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Thermo Scientific™ DpnI (10 U/μ L)

Artikelnummer. 10669500 Shop alle Thermo Scientific produkter
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Mængde:
1.000 enheder
2500 U
500 units
Pakningsstørrelse:
1000 enheder
500 enheder
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10669500 500 units 500 enheder
10304240 2500 U -
10140690 1.000 enheder 1000 enheder
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To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”27968” to get your promotional price
Artikelnummer. 10669500 Leverandør Thermo Scientific™ Leverandørnr. ER1701

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292.68 DKK valid until 2026-03-20
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Denne vare kan ikke returneres. Se returpolicy
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”27968” to get your promotional price

Beskrivelse

Skær ved Gm6A^TC-steder med DpnI-restriktionsenzym, som fungerer bedst ved 37 °C i Tango-buffer (Isoschizomerer: MalI).

Konventionelle restriktionsendonukleaser er en stor samling af højkvalitets restriktionsenzymer, optimeret til at arbejde i en af bufferne i Five Buffer System. Derudover leveres den universelle Tango-buffer for bekvemmelighed ved dobbeltfordøjelser. Alle enzymerne udviser 100 % aktivitet under de anbefalede buffer- og reaktionsbetingelser. For at sikre ensartet ydeevne, Thermo Scientific restriktionsenzymreaktionsbuffere indeholder forblandet BSA, som øger stabiliteten af mange enzymer og binder kontaminanter, der kan være til stede i DNA-præparater.

5’G Am6↓T C 3’
3’C T ↑Am6 G 5’

Thermo Scientific DpnI restriction enzyme recognizes Gm6A^TC sites and cuts best at 37°C in Tango buffer (isoschizomers: MalI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion.

In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Features

  • Superior quality—stringent quality control and industry leading manufacturing process
  • Convenient color-coded Five Buffer System
  • Includes universal Tango buffer for double-digestions
  • BSA premixed in reaction buffers
  • Wide selection of restriction endonuclease specificities

Applications

  • Molecular cloning
  • Restriction site mapping
  • Genotyping
  • Southern blotting
  • Restriction fragment length polymorphism (RFLP)
  • SNP

Note: DpnI requires the presence of N6-methyladenine within the recognition sequence to cleave DNA. DNA purified from a dam+ strain will be a substrate for DpnI. DpnI will only cleave fully-adenomethylated dam sites. Hemi-adenomethylated dam sites DpnI cleaves 60X more slowly. DpnI, Bsp143I, and MboI all recognize the same sequence, but have different methylation sensitivities and cleavage sites. Assayed using pBR322 DNA. For methylation sensitivity, refer to product specifications.

TRUSTED_SUSTAINABILITY

Tekniske data

Koncentration 10 U/μL
Methyleringsfølsomhed Dam Methylation-Sensitive, Not CpG Methylation-Sensitive, Not Dcm Methylation-Sensitive
Enzym Dpn I
Kompatibel buffer 10X Buffer Tango
Følsom over for varmeinaktivering Yes
Optimal reaktionstemperatur 37°C
Type IIS RE No
Mængde 500 units
Produkttype Restriction Enzyme
Forskningskategori Traditional Cloning

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