
Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
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Thermo Scientific™ Potassium phosphate, 0.2M buffer soln., pH 7.5
CAS: 7778-77-0 Molecular Formula: H2KO4P Molecular Weight (g/mol): 136.08 MDL Number: MFCD00011401 MFCD00147253 InChI Key: GNSKLFRGEWLPPA-UHFFFAOYSA-M IUPAC Name: potassium dihydrogen phosphate SMILES: [K+].OP(O)([O-])=O
CAS | 7778-77-0 |
---|---|
Molecular Weight (g/mol) | 136.08 |
MDL Number | MFCD00011401 MFCD00147253 |
SMILES | [K+].OP(O)([O-])=O |
IUPAC Name | potassium dihydrogen phosphate |
InChI Key | GNSKLFRGEWLPPA-UHFFFAOYSA-M |
Molecular Formula | H2KO4P |
Invitrogen™ 20X Bolt™ MES SDS Running Buffer
Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats

For Use With (Equipment) | Bolt |
---|---|
Concentration | 20X |
Product Line | Bolt |
Thermo Scientific™ Dry-blend Buffered Packs
Our quality control ensures that every pack of dry-blend buffers yields consistent solutions without weighing or pH adjustment. Simply empty contents of one foil envelope pack into a beaker, add appropriate solvents, and stir to dissolve.

Form | Powder |
---|---|
Product Line | BupH™ |
Thermo Scientific™ Pierce™ Concentrated Buffer Stocks (10X and 20X)
Save time using Pierce Concentrated Buffer stock solutions (10X and 20X) with simple dilutions to expedite your experiment without sacrificing quality or consistency.

Thermo Scientific Chemicals HEPES, 1.0M buffer soln., pH 7.5
CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N PubChem CID: 23831 ChEBI: CHEBI:42334 SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1

PubChem CID | 23831 |
---|---|
CAS | 7365-45-9 |
Molecular Weight (g/mol) | 238.30 |
ChEBI | CHEBI:42334 |
MDL Number | MFCD00006158 |
SMILES | OCCN1CCN(CCS(O)(=O)=O)CC1 |
InChI Key | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
Molecular Formula | C8H18N2O4S |
TAE Buffer, Tris-Acetate-EDTA, 50X Solution, Electrophoresis, Fisher BioReagents™
Tris-Acetate-EDTA, CAS Number-77-86-1, 60-00-4, 6850-28-8, TAE, 4L, Gray, Tris (24%), Acetic Acid (5.0%), and EDTA (<2%)., DNase free, Pass Test, Filtered through a 0.2-micron filter., Electrophoresis, 50X Solution, Poly CUBE, Liquid, Protease free, DNase-, RNase- and Protease-Free, RT

Name Note | 50X Solution |
---|---|
Concentration or Composition (by Analyte or Components) | Tris (24%), Acetic Acid (5.0%), and EDTA (<2%). |
CAS | 6850-28-8 |
Protease | Protease free |
Physical Form | Liquid |
Grade | Electrophoresis |
Synonym | TAE |
Purity Grade Notes | DNase-, RNase- and Protease-Free |
DNase | DNase free |
Recommended Storage | RT |
Filtered Through | Filtered through a 0.2-micron filter. |
ChemAlert Storage Symbol | Gray |
Thermo Scientific Chemicals Krebs-Ringer Solution, HEPES-buffered
Standard physiological buffer that is suitable for various calcium imaging experiments. It is also used as a preserving agent.

Solubility Information | Not miscible or difficult to mix in water. |
---|---|
Packaging | Plastic bottle |
Physical Form | Liquid |
Chemical Name or Material | Krebs-Ringer Solution |
TSCA | Yes |
Recommended Storage | Ambient temperatures |
Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer (10X)
Formulated for separation of proteins, in their denatured state, on Novex Tris-Glycine gels

Chemical Name or Material | Running Buffer |
---|---|
Concentration | 10X |
Product Line | Novex |
Invitrogen™ Novex™ Tricine SDS Running Buffer (10X)
Novex Tricine SDS Running Buffer (10X) is formulated for separation of proteins in their denatured state on tricine gels.

Thermo Scientific Chemicals TRIS-buffered saline (TBS, 20X) pH 7.4
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00132476 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

PubChem CID | 6503 |
---|---|
CAS | 77-86-1 |
Molecular Weight (g/mol) | 121.136 |
ChEBI | CHEBI:9754 |
MDL Number | MFCD00132476 |
SMILES | C(C(CO)(CO)N)O |
IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
Molecular Formula | C4H11NO3 |
Invitrogen™ NuPAGE™ MOPS SDS Running Buffer (20X)
Made with high-purity reagents and are strictly quality controlled.

Formulation | 50 mM MOPS, 50 mM Tris Base, 0.1% SDS, 1 mM EDTA, pH 7.7, 0.01-0.09% N,N-dimethylformamide |
---|---|
Physical Form | Liquid |
pH | 7.7 |
Chemical Name or Material | Running Buffer |
Recommended Storage | Contents: NuPAGE™ MOPS SDS Running Buffer (20X) Storage: +4°C to 25°C |
Concentration | 20X |
Product Line | NuPAGE |
Thermo Scientific Chemicals HEPES, 1.0M buffer soln., pH 8.0
CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1

PubChem CID | 23831 |
---|---|
CAS | 7365-45-9 |
Molecular Weight (g/mol) | 238.30 |
ChEBI | CHEBI:42334 |
MDL Number | MFCD00006158 |
SMILES | OCCN1CCN(CCS(O)(=O)=O)CC1 |
IUPAC Name | 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid |
InChI Key | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
Molecular Formula | C8H18N2O4S |
TAE Buffer, Tris-Acetate-EDTA, 10X Solution, Electrophoresis, Fisher BioReagents

Name Note | 10X Solution |
---|---|
Concentration or Composition (by Analyte or Components) | Tris (<5.0%), Acetic Acid (1.0%), and EDTA (<1%). |
CAS | 77-86-1 |
Protease | Protease free |
Physical Form | Liquid |
Chemical Name or Material | Tris-Acetate-EDTA |
Grade | Electrophoresis |
Synonym | TAE |
Purity Grade Notes | DNase-, RNase- and Protease-Free |
DNase | DNase free |
Recommended Storage | RT |
Filtered Through | Filtered through a 0.2-micron filter. |
ChemAlert Storage Symbol | Gray |
Invitrogen™ 20X Bolt™ MOPS SDS Running Buffer
Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats

For Use With (Equipment) | Bolt |
---|---|
Physical Form | Liquid |
Chemical Name or Material | Running Buffer |
Concentration | 20X |
Product Line | Bolt |