Biological Buffers

Invitrogen™ Novex™ NuPAGE™ Antioxidant

Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis 15ML NUPAGE ANTIOXIDANT

Invitrogen™ Novex™ NuPAGE™ MES SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled NUPAGE MES 20X RUN BUF

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

NUCLEASE-FREE WATER 100 MLAmbion® Nuclease-free water has not been treated

Invitrogen™ Novex™ NuPAGE™ MOPS SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled NUPAGE MOPS 20XRUN BUF

Invitrogen™ Novex™ NuPAGE™ Sample Reducing Agent (10X)

Ready-to-use reagent to reduce protein samples for protein gel electrophoresis SAMPLE REDUCING AGENT (10X), LIQUID NUPAGE(R)10mL Store at 2-8C

Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer (10X)

Formulated for separation of proteins, in their denatured state, on Novex Tris-Glycine gels TS SDS 10X RUN BUF, 5L

Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X)

LDS SAMPLE BUFFER (4X), NUPAGE(R) 250ML STORE AT2-8C

Invitrogen™ Salmon Sperm DNA, sheared (10 mg/mL)

Blocks non-specific hybridization SALMON SPERM DNA (SHARED)10 tubes

Invitrogen™ tYeast RNA (10mg/mL)

Co-precipitant to aid in nucleic acid recovery 500 UL YEAST TRNA (10 MG/ML) 500µL STORE AT -20 C

Invitrogen™ Novex™ pH 3-10 IEF Buffer Kit

Optimized for using with Novex™ pH 3-10 IEF gels IEF BUFFER KIT PH 3 TO 10 00

Thermo Scientific™ Blocker™ BLOTTO Blocking Buffer

Block membranes, plates and other samples with this ready-to-use 5% solution of nonfat powdered milk in TBS for Western blot, ELISA and other detection methods. BLOCKER BLOTTO IN TBS, 1 L, MA: 375304°C

Invitrogen™ PBS Phosphate-Buffered Saline (10X) pH 7.4

Dilute to desired concentration 10X PBS BUFFER, PH 7.4 500 MLMolecular biology grade, Ambion® PBS 10X, pH 7.4

Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

Nucleaic acid purfication PHENOL/CHLOROFORM/ALCOHOL

Thermo Scientific™ Restore™ Plus Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates. X2 RESTORE(TM) PLUS WESTERN BLOT STRIPPING BUFFER30mL

Invitrogen™ Novex™ 20X Bolt™ MES SDS Running Buffer

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats 500 ML BOLT MES SDS RUNNING BUFFR 20X

Thermo Scientific™ SuperBlock™ Blocking Buffers

Block membranes, plates and tissue samples with these optimized solutions containing a protein that provides fast and effective blocking for Western blot, ELISA and IHC. 1 LT T20 (PBS) BLOCKING BUFFER, SUPERBLOCK 1LStore in fridge at 4C

Invitrogen™ Novex™ NuPAGE™ Transfer Buffer (20X)

Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting NP TRANSFER BUF (20X)

Invitrogen™ Novex™ NuPAGE™ Tris-Acetate SDS Running Buffer (20X)

Formulated for separation of proteins, in their denatured state, on NuPAGE Novex Tris-Acetate gels NUPAGE TA SDS RNBUF (20X)

Invitrogen™ Novex™ Tris-Glycine SDS Sample Buffer (2X)

Use with Novex Tris-Glycine Gels TRISGLY SDS SAM BUF (2X)20ML

Invitrogen™ Phenol:Chloroform:IAA, 25:24:1, pH 6.6

Ready-to-use, safer than preparing crystalline phenol solutions 400 ML PHENOL:CHLOROFORM:IAA, 25:24:1, PH 6.6(raise to pH 7.9 with included buffer) 400mL

Thermo Scientific™ Pierce™ ELISA Blocker Blocking Buffer

Increases signal-to-noise ratio and minimizes background 1 LT ELISA BLOCKER BLOCKING BUFFER 1L

Merck™ ReBlot Plus Kit and Reagents

Blots can be stripped of antibodies in approximately 15 minutes at room temperature 50ML Re-Blot Plus Mild Solution (10X)

Laemmli SDS Sample Buffer, non-reducing, 6X

For protein sample preparation to be used in the Laemmli SDS-PAGE system 50ML Laemmli SDS sample buffer, non-reducing (6X) 50ml

MES hydrate, 99+%, Acros Organics™

CAS: 1266615-59-1 Molecular Formula: C6H13NO4S·xH2O MDL Number: MFCD00149409 Synonym: 4-Morpholinoethanesulfonic acid hydrate, MES 2.5KG MES hydrate, 99+%, for biochemistry

Tricine (White Crystals), Fisher BioReagents

100GR Tricine (White Crystals),

Alfa Aesar™ PIPES lysis buffer with Triton™ X-100

500ML PIPES lysis buffer with Triton]r X-100 500ml

Invitrogen™ Acid-Phenol:Chloroform, pH 4.5 (with IAA, 125:24:1)

Premixed, safer, and easier alternative to preparing crystalline phenol solutions 400 ML ACID-PHENOL:CHLOROFORM, PH 4.5 (WITH IAA,125:24:1) 400mL Store at 4°C

Thermo Scientific™ Pierce™ ELISA Wash Buffers

Concentrated wash buffer for efficient use of storage in the lab ELISA WASH BUFFER, 30X 50ML

Invitrogen™ UltraPure™ Dithiothreitol

High and low concentration applications DITHIOTHREITOL (DTT)

Invitrogen™ TE, pH 8.0

Ready-to-use, certified RNase-free 1 LT TE, PH 8.0 1L STORE AT ROOM TEMPERATURE

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