Biological Buffers

Invitrogen™ Novex™ NuPAGE™ MES SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled NUPAGE MES 20X RUN BUF

Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X)

LDS SAMPLE BUFFER (4X), NUPAGE(R) 250ML STORE AT2-8C

Invitrogen™ Novex™ NuPAGE™ Sample Reducing Agent (10X)

Ready-to-use reagent to reduce protein samples for protein gel electrophoresis SAMPLE REDUCING AGENT (10X), LIQUID NUPAGE(R)10mL Store at 2-8C

Invitrogen™ Novex™ NuPAGE™ MOPS SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled NUPAGE MOPS 20XRUN BUF

Invitrogen™ Novex™ NuPAGE™ Antioxidant

Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis 15ML NUPAGE ANTIOXIDANT

Invitrogen™ Novex™ Tris-Glycine SDS Sample Buffer (2X)

Use with Novex Tris-Glycine Gels TRISGLY SDS SAM BUF (2X)20ML

Invitrogen™ Salmon Sperm DNA, sheared (10 mg/mL)

Blocks non-specific hybridization SALMON SPERM DNA (SHARED)10 tubes

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

500 ML NUCLEASE-FREE WATER (NOT DEPC-TREATED) (1x 500mL) 500mL Store at room temperature

Invitrogen™ PBS Phosphate-Buffered Saline (10X) pH 7.4

Dilute to desired concentration 10X PBS BUFFER, PH 7.4 500 MLMolecular biology grade, Ambion® PBS 10X, pH 7.4

Invitrogen™ Novex™ NuPAGE™ MOPS SDS Buffer Kit (for Bis-Tris Gels)

All buffers are made with high-purity reagents and are strictly quality controlled MOPS SDS BUFFER KIT NUPAGE(R) FOR BIS-TRIS GELS 00

Lonza™ AccuGENE™ PBS

Ready to use solutions ideal for a wide range of molecular biology applications AccuGENE 1X PBS 1L

Invitrogen™ Novex™ 20X Bolt™ MES SDS Running Buffer

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats 500 ML BOLT MES SDS RUNNING BUFFR 20X

Invitrogen™ Novex™ NuPAGE™ Transfer Buffer (20X)

Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting NP TRANSFER BUF (20X)

Invitrogen™ Novex™ pH 3-10 IEF Buffer Kit

Optimized for using with Novex™ pH 3-10 IEF gels IEF BUFFER KIT PH 3 TO 10 00

Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

Nucleaic acid purfication PHENOL/CHLOROFORM/ALCOHOL

Invitrogen™ Proteinase K Solution (20mg/mL)

General digestion of proteins PROTEINASE K (20MG/ML)5X1.25ML5 tubes

Thermo Scientific™ Pierce™ Protein-Free Blocking Buffers

Block membranes, plates and other samples with these ready-to-use PBS and TBS formulations of a non-protein compound to yield extremely low background. 100mL Protein-Free (PBS) blocking buffer

Thermo Scientific™ Blocker™ BLOTTO Blocking Buffer

Block membranes, plates and other samples with this ready-to-use 5% solution of nonfat powdered milk in TBS for Western blot, ELISA and other detection methods. BLOCKER BLOTTO IN TBS, 1 L, MA: 375304°C

Invitrogen™ TE, pH 8.0

Ready-to-use, certified RNase-free 1 LT TE, PH 8.0 1L STORE AT ROOM TEMPERATURE

Thermo Scientific™ Restore™ Plus Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates. X2 RESTORE(TM) PLUS WESTERN BLOT STRIPPING BUFFER30mL

Invitrogen™ Tris (1M), pH 8.0

Ready-to-use, certified RNase-free 500 ML 1 M TRIS, PH 8.0 500ML STORE AT ROOM TEMPERature

Invitrogen™ Ammonium Acetate (5M)

Ready-to-use, certified RNase-free 100 ML 5 M AMMONIUM ACETATE 100ML STORE AT ROOMtemperature

Invitrogen™ Novex™ IEF Cathode Buffer pH 3-10 (10X)

An optimized pre-mixed IEF cathod buffer IEF CATHODE PH3-10(10X)125MLNovex® IEF Cathode Buffer pH 3-10 (10X) is

Invitrogen™ AccuPrime™ Pfx DNA Polymerase

Provides higher fidelity than Pfu DNA polymerase 1000 RXN PFX DNA POLYMERASE ACCUPRIME(TM) 1000reactions Store at -20C

Invitrogen™ Novex™ Tris-Glycine Native Running Buffer (10X)

Use with NuPAGE Novex Tris-Acetate gels TRISGLY NATIVE RUN BUF 500ML

Alfa Aesar™ PIPES lysis buffer with Triton™ X-100

500ML PIPES lysis buffer with Triton]r X-100 500ml

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed. 500 ML WESTERN BLOT STRIPPING REAGENT, RESTORE500mL

Invitrogen™ UltraPure™ Dithiothreitol

High and low concentration applications DITHIOTHREITOL (DTT)

Invitrogen™ Novex™ Tricine SDS Buffer Kit

Use with Novex Tricine Gels TRICINE SDS BUFFER KIT 00

GE Healthcare Pharmalyte™ Carrier Ampholytes

Use in Isoelectric focusing. GE Healthcare Pharmalyte™ Carrier Ampholytes prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals. 25ML Pharmalyte, broad range pH 2.5 - 5Store in Fridge at 4C

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