Biological Buffers

Invitrogen™ PBS Phosphate-Buffered Saline (10X) pH 7.4

Dilute to desired concentration 10X PBS BUFFER, PH 7.4 1000 MLsolution, 1000 ml

Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X)

LDS SAMPLE BUFFER (4X), NUPAGE(R) 250ML STORE AT2-8C

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

NUCLEASE-FREE WATER 100 MLAmbion® Nuclease-free water has not been treated

Invitrogen™ Novex™ NuPAGE™ Sample Reducing Agent (10X)

Ready-to-use reagent to reduce protein samples for protein gel electrophoresis SAMPLE REDUCING AGENT (10X), LIQUID NUPAGE(R)10mL Store at 2-8C

Invitrogen™ Novex™ NuPAGE™ MES SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled NUPAGE MES 20X RUN BUF

Lonza™ AccuGENE™ PBS

AccuGENE 10X PBS FP=1 l

Thermo Scientific™ 6X DNA Loading Dye

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye. X5 6X DNA LOADING DYE, COMPOSITION: 10MM TRIS-HCL(pH7.6), 0.03% bromophenol blue, 0.03% xylene

Thermo Scientific™ Restore™ Plus Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates. X2 RESTORE(TM) PLUS WESTERN BLOT STRIPPING BUFFER30mL

Invitrogen™ Novex™ NativePAGE™ Sample Prep Kit

Includes sample preparation reagents for native gel electrophoresis NATIVEPAGE SAMPLE PREP KITThe NativePAGE Sample Prep Kit includes sample

Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

Nucleaic acid purfication PHENOL/CHLOROFORM/ALCOHOL

Invitrogen™ Novex™ NativePAGE™ Running Buffer Kit

Contains the NativePAGE Running Buffer (20X) NATIVEPAGE RUNNING BUFFER KIT 0 DDNSA # DAYS =155 CDNSA # days = 185 Special Storage Details =

Invitrogen™ Novex™ NuPAGE™ MOPS SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled NUPAGE MOPS 20XRUN BUF

Invitrogen™ RT-PCR Grade Water, Not DEPC-treated

RT-PCR GRADE WATER 10 X 1.7MLAmbion® RT-PCR grade water is tested for

Invitrogen™ TE, pH 7.0

Nuclease-free, ultrapure solution for RNA samples TE BUFFER 10 X 1.0 MLAmbion® TE buffer is a commonly recommended

Invitrogen™ Novex™ IEF Anode Buffer (50X)

An optimized pre-mixed IEF anode buffer IEF ANODE BUFFER (50X) 100MLNovex® IEF Anode Buffer (50X) is optimized

Invitrogen™ EDTA (0.5M), pH 8.0

Ready-to-use, certified RNase-free 500 ML 0.5 M EDTA, PH 8.0 500ML STORE AT ROOMtemperature

Invitrogen™ Novex™ NuPAGE™ Antioxidant

Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis 15ML NUPAGE ANTIOXIDANT

Thermo Scientific™ SuperBlock™ Blocking Buffers

Block membranes, plates and tissue samples with these optimized solutions containing a protein that provides fast and effective blocking for Western blot, ELISA and IHC. SUPERBLOCK BLOCKING BUFF IN TBS 1LTemperature Store in Fridge at 4C

Invitrogen™ Novex™ NuPAGE™ Transfer Buffer (20X)

Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting NP TRANSFER BUF (20X)

Invitrogen™ AccuPrime™ SuperMix II

Designed for amplification of genomic DNA templates 200bp-4kb 1000 RXN SUPERMIX II 1000 REACTIONS STORE AT -20C

Invitrogen™ DEPC-Treated Water

DEPC-TREATED WATER 5 X 100 MLAmbion® DEPC-treated water is certified

Invitrogen™ Acid-Phenol:Chloroform, pH 4.5 (with IAA, 125:24:1)

Premixed, safer, and easier alternative to preparing crystalline phenol solutions 400 ML ACID-PHENOL:CHLOROFORM, PH 4.5 (WITH IAA,125:24:1) 400mL Store at 4°C

GE Healthcare Pharmalyte™ Carrier Ampholytes

Use in Isoelectric focusing. GE Healthcare Pharmalyte™ Carrier Ampholytes prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals. 25 ML CARRIER AMPHOLYTE, PHARMALYTE 6.7 TO 7.725mL Store in Fridge at 4C

Thermo Scientific™ Phusion Buffers

Choose between two buffer packs to optimize high-fidelity PCR or difficult or long template amplification using Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases. DETERGENT-FREE GC BUFFER 6 MLML

Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer (10X)

Formulated for separation of proteins, in their denatured state, on Novex Tris-Glycine gels NOVEX TRIS-GLYCINE SDS RUNNING BUFFER (10X DENATURing) . store at room temperature

Invitrogen™ Novex™ 4X Bolt™ LDS Sample Buffer

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats 10 ML BOLT LDS SAMPLE BUF (4X)

Invitrogen™ GlycoBlue™ Coprecipitant (15mg/mL)

Blue dye increases pellet visibility GLYCO BLUE COPRECIP 5 X 300ULAmbion® GlycoBlue consists of a blue dye

Thermo Scientific™ Pierce™ StartingBlock™ Blocking Buffers

Block membranes, plates and other samples with these ready-to-use PBS or TBS formulations of a special protein blocker for exceptional nonspecific blocking and broad compatibility. 100mL StartingBlock (TBS) blocking buffer

Thermo Scientific™ Pierce™ Lane Marker Sample Buffers

Ready-to-use 5X SDS-PAGE sample loading buffers available in reducing and nonreducing formulations, both with a pink dye buffer-front marker. 5 ML LANE MARKER NON-REDUCING SAMPLE BUFFER (5X)5mL

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