Biological Buffers

Invitrogen™ Novex™ NuPAGE™ Sample Reducing Agent (10X)

Ready-to-use reagent to reduce protein samples for protein gel electrophoresis SAMPLE REDUCING AGENT (10X), LIQUID NUPAGE(R)10mL Store at 2-8C

Invitrogen™ Novex™ NuPAGE™ MES SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled NUPAGE MES 20X RUN BUF

Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X)


Invitrogen™ Novex™ Tricine SDS Sample Buffer (2X)

Use with Novex Tricine Gels NOVEX(R) TRICINE SDS RUNNING BUFFER (2X), LIQUID20mL Store at 2-8C

Invitrogen™ Novex™ NuPAGE™ MOPS SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled NUPAGE MOPS 20XRUN BUF

Thermo Scientific™ Phusion Buffers

Choose between two buffer packs to optimize high-fidelity PCR or difficult or long template amplification using Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases. DETERGENT-FREE HF BUFFER 6 ML

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

Storage Requirements: Store at room temperature. NUCLEASE-FREE WATER 5 X 100 MLAmbion® Nuclease-free water has not been treated

Invitrogen™ Novex™ NuPAGE™ Antioxidant

Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis 15ML NUPAGE ANTIOXIDANT

Invitrogen™ Novex™ Digitonin (5%)

A detergent solution used to prepare native protein samples for use with the NativePAGE™ Gel System 5% DIGITONINthe NativePAGE Gel System

Invitrogen™ tYeast RNA (10mg/mL)

Co-precipitant to aid in nucleic acid recovery 500 UL YEAST TRNA (10 MG/ML) 500µL STORE AT -20 C

Invitrogen™ PBS Phosphate-Buffered Saline (10X) pH 7.4

Dilute to desired concentration 10X PBS BUFFER, PH 7.4 500 MLMolecular biology grade, Ambion® PBS 10X, pH 7.4

Thermo Scientific™ Pierce™ Lane Marker Sample Buffers

Ready-to-use 5X SDS-PAGE sample loading buffers available in reducing and nonreducing formulations, both with a pink dye buffer-front marker. 5 ML LANE MARKER NON-REDUCING SAMPLE BUFFER (5X)5mL

Thermo Scientific™ Pierce™ StartingBlock™ Blocking Buffers

Block membranes, plates and other samples with these ready-to-use PBS or TBS formulations of a special protein blocker for exceptional nonspecific blocking and broad compatibility. 100mL StartingBlock (TBS) blocking buffer

Thermo Scientific™ Restore™ Plus Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates. X2 RESTORE(TM) PLUS WESTERN BLOT STRIPPING BUFFER30mL

Invitrogen™ DEPC-Treated Water

Storage Requirements: Store at room temperature. 500 ML DEPC-TREATED WATER (1 X 500ML) 500ML STOREat room temperature

Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

Nucleaic acid purfication PHENOL/CHLOROFORM/ALCOHOL

GE Healthcare Pharmalyte™ Carrier Ampholytes

Use in Isoelectric focusing. GE Healthcare Pharmalyte™ Carrier Ampholytes prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals. 25 ML CARRIER AMPHOLYTE, PHARMALYTE 4.2 TO 4.925mL Store in Fridge at 4C

Invitrogen™ Novex™ IEF Anode Buffer (50X)

An optimized pre-mixed IEF anode buffer IEF ANODE BUFFER (50X) 100MLNovex® IEF Anode Buffer (50X) is optimized

Invitrogen™ Proteinase K Solution (20mg/mL)

General digestion of proteins PROTEINASE K (20MG/ML)5X1.25ML5 tubes

Invitrogen™ Novex™ NuPAGE™ Transfer Buffer (20X)

Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting 1 LT Transfer buffer NuPAGE(r) (20X), liquid 1L

Invitrogen™ TE, pH 7.0

Nuclease-free, ultrapure solution for RNA samples TE BUFFER 10 X 1.0 MLAmbion® TE buffer is a commonly recommended

Invitrogen™ Novex™ IEF Cathode Buffer pH 3-10 (10X)

An optimized pre-mixed IEF cathod buffer IEF CATHODE PH3-10(10X)125MLNovex® IEF Cathode Buffer pH 3-10 (10X) is

Invitrogen™ Novex™ NativePAGE™ Sample Prep Kit

Includes sample preparation reagents for native gel electrophoresis NATIVEPAGE SAMPLE PREP KITThe NativePAGE Sample Prep Kit includes sample

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed. 30 ML WESTERN STRIPPING REAGENT, RESTORE 30ML

MES hydrate, 99+%, Acros Organics™

Chemical Name or Material: MES hydrate Name Note: 99% CAS: 1266615-59-1 Purity Grade: For biochemistry Assay: ≥99% Assay Percent Range: 99+% Molecular Formula: C6H13NO4S·xH2O Formula Weight: 195.23 MDL Number: MFCD00149409 Synonym: 4-Morpholinoethanesulfonic acid hydrate, MES 100GR MES hydrate, 99+%, for biochemistry

Invitrogen™ GlycoBlue™ Coprecipitant (15mg/mL)

Blue dye increases pellet visibility GLYCO BLUE COPRECIP 5 X 300ULAmbion® GlycoBlue consists of a blue dye

Invitrogen™ UltraPure™ Buffer-Saturated Phenol

Buffer for nucleic acid purification BUFFER SATURATED PHENOL

Invitrogen™ UltraPure™ Guanidine Hydrochloride

Highly concentrated protein denaturant GUANIDINE HYDROCHLORIDE

Thermo Scientific™ Blocker™ BLOTTO Blocking Buffer

Block membranes, plates and other samples with this ready-to-use 5% solution of nonfat powdered milk in TBS for Western blot, ELISA and other detection methods. BLOCKER BLOTTO IN TBS, 1 L, MA: 375304°C