DNA Reagents

Chemical reagents and other products used for DNA-related techniques and processes such as PCR cloning, DNA sequencing, DNA cloning and mapping, mutagenesis, DNA synthesis, etc.; includes kits, columns, buffers, labelling technology, and others.

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Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, without competent cells

Pricing and Availability
Specifications

Promoter	SP6
Content And Storage	Box 1: • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored.
Antibiotic Resistance Bacterial	Kanamycin (KanR), Zeocin™ (ZeoR)
Format	Kit
Product Type	PCR Cloning Kit
Cloning Method	Blunt TOPO™

Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit

The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.

Pricing and Availability
Specifications

Format	12-well Strips,8-well Strip,96- or 384-well Plate
Product Type	Sequencing Buffer
For Use With (Equipment)	SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐well and 384‐well Thermal Cyclers
Technique	Fluorescent Dye Terminator Sequencing
Product Line	BigDye™ Terminator

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Thermo Scientific™ CloneJET PCR Cloning Kit

Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.

Pricing and Availability
Specifications

Antibiotic Resistance Bacterial	Ampicillin (AmpR)
Format	Kit
Sample Type	PCR Products
Cloning Method	Restriction Enzyme⁄ MCS, Blunt PCR
For Use With (Application)	PCR Cloning
Vector	pJET1.2/blunt
Product Line	CloneJET

Applied Biosystems™ BigDye™ Terminator v1.1 & v3.1 5X Sequencing Buffer

This sequencing buffer is optimized for use with BigDye™ Terminator v1.1 and v3.1 Cycle Sequencing Kits.

Pricing and Availability
Specifications

Shipping Condition	Room Temperature
Buffer	Sequencing Buffer
Content And Storage	Store at 2°C to 8°C.
Form	Liquid
Concentration	5X
For Use With (Application)	Standard Sequencing

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Thermo Scientific™ pUC19 DNA

Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 10⁶ Da isolated from E.coli.

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Invitrogen™ GeneArt™ Gibson Assembly HiFi Master Mix

GeneArt Gibson Assembly HiFi Master Mix enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Content And Storage	Includes master mix, positive control, and water and accommodates the use of your own competent cells.
Workflow Step	DNA Assembly
Number of Fragments	Up to 6 fragments
Product Type	HiFi Master Mix
Control Type	Positive Control
Cloning Method	Seamless cloning
For Use With (Application)	Seamless Cloning & Genetic Assembly
Product Line	GENEART™

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Thermo Scientific™ pENTR™/D-TOPO™ Cloning Kit

Utilize a highly efficient, 5-minute cloning strategy (TOPO™ Cloning) to directionally clone a blunt-end PCR product into a vector for entry into the Gateway™ System or the MultiSite Gateway™ System

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Applied Biosystems™ Sequencing Standard, BigDye™ Terminator v3.1, for 3500/SeqStudio™ Flex

The DNA contained in the preparation has been lyophilized to maximize stability.The two hydrolytic enzymes used in this kit, shrimp alkaline phosphatase and exonuclease I, effectively remove excess dNTPs and primers.

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Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, without competent cells

Pricing and Availability
Specifications

Antibiotic Resistance Bacterial	Ampicillin (AmpR), Kanamycin (KanR)
Format	Kit
Product Type	Cloning Kit
Cloning Method	TOPO™-TA
Product Line	TOPO™

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Thermo Scientific™ pBR322 Plasmid DNA

Optimize cloning with Thermo Scientific™ pBR322 DNA, a 4361 bp double-stranded closed circular medium copy plasmid DNA.

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Thermo Scientific™ Rapid DNA Ligation Kit

Enable fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.

Pricing and Availability
Specifications

Product Type	Rapid DNA Ligation Kit
Enzyme	DNA Ligase

Applied Biosystems™ 3730 Running Buffer (10X)

The concentrated Running Buffer is used for electrophoresis on Applied Biosystems™3730 and 3730XL DNA Analyzers.

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Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ TOP10 E. coli

Delivers simple, 5-minute cloning and access to Gateway recombination cloning vector platform

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Invitrogen™ GeneArt™ Gibson Assembly HiFi Cloning Kit, chemically competent cells

The GeneArt Gibson Assembly HiFi Cloning Kit enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.

Pricing and Availability

Invitrogen™ TOPO™ XL-2 Complete PCR Cloning Kit, with One Shot™ OmniMAX™ 2 T1^R Chemically Competent E. coli Cells

Provides all the necessary elements for efficient cloning of extra-long PCR products (up to 13 kb)

Pricing and Availability
Specifications

Shipping Condition	Approved for shipment at Room Temperature or on Wet or Dry Ice
Promoter	T3, T7
Content And Storage	Box 1: Store at -5 to -30°C. • pCR XL-2-TOPO vector • Salt Solution • T3 Primer • T7 Primer • XL Control PCR Template • XL Control PCR Primers • Platinum SuperFi Green PCR Master Mix • SuperFi GC Enhancer • Nuclease-free water Box 2: Store at room temperature. • Gel Solubilization Buffer (L3) • Binding Buffer (B2) • Wash Buffer (W1) • Elution Buffer (E1) • PureLink Clean-up Spin Columns (in Wash Tubes) • PureLink Recovery/Elution Tubes Box 3: • One Shot OmniMAX 2 T1R chemically competent E. coli • S.O.C. Medium • pUC 19 Control DNA Store competent cells and pUC19 at -65 to -85°C.
Antibiotic Resistance Bacterial	Ampicillin (AmpR), Kanamycin (KanR)
Format	Kit
Product Type	Cloning Kit
Cloning Method	Zero Blunt TOPO
Bacterial or Yeast Strain	OmniMAX2
For Use With (Application)	PCR Cloning
Vector	pCR-XL-2-TOPO
Product Line	TOPO™
Cell Type	Chemically Competent E. coli

DNA Reagents

DNA Reagents

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Applied Biosystems™ 3730 Running Buffer (10X) Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

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Applied Biosystems™ 3730 Running Buffer (10X)