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Optimize cloning with Thermo Scientific™ pBR322 DNA, a 4361 bp double-stranded closed circular medium copy plasmid DNA.
For expression of hROR gamma (t) [del. 5-8] protein, which is a novel hROR gamma (t) isoform
A fast way to purify transfection-grade plasmid DNA using mini-format technology to reduce endotoxins from bacterial lysates. Complete kit includes mini spin columns for an ultra-fast workflow.
Content And Storage | Room temperature (18 - 25 °C) |
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Final Product Type | Transfection-grade plasmid DNA |
Size | < 15 kbp |
Isolation Technology | Silica Membrane and Endotoxin-reduction Technology |
Purity | A260/A280: 1.80 - 1.85 |
Sample Volume | Up to 5 mL |
For Use With (Application) | Rapid purification of plasmids for transfection |
Test Time | 14 min/6 preps |
Includes | NucleoSpin Plasmid Transfection-grade Columns, Collection Tubes, Buffers, RNase A |
Format | Mini Spin Column |
For Use With (Equipment) | Centrifuge, Vacuum manifold (e.g. NucleoVac 96) |
Efficiency | Binding capacity: 35 ÎĽg |
Sample Type | Bacterial Culture (E. coli, High-copy Plasmids) |
Yield | 15 - 30 ÎĽg |
Shelf Life | At least 1 year |
Elution Volume | Elution Volume: 30 - 50 ÎĽL |
Bacterial Endotoxins | <50 EU/ng DNA |
For expression of hRUNX1 protein in mammalian cells, induction of FOXP3 promoter activity
Mammalian expression vector construct, pCMV/hRUNX1, containing coding sequence of human Runt-related transcription factor 1
A fast way to purify transfection-grade plasmid DNA using mini-format technology to reduce endotoxins from bacterial lysates. Full and Core kits utilize a 96-well plate format for an efficient high-throughput process.
Content And Storage | Room temperature (18 - 25 °C) |
---|---|
Final Product Type | Transfection-grade plasmid DNA |
Size | < 15 kbp |
Isolation Technology | Silica Membrane and Endotoxin-reduction Technology |
Purity | A260/A280: 1.80 - 1.85 |
Sample Volume | Up to 5 mL |
For Use With (Application) | Rapid purification of plasmids for transfection |
Test Time | 45 min/plate |
Format | 96-well Plate |
For Use With (Equipment) | Centrifuge, Vacuum manifold (e.g. NucleoVac 96) |
Efficiency | Binding capacity: 20 ÎĽg |
Sample Type | Bacterial Culture (E. coli, High-copy Plasmids) |
Yield | 5 - 20 ÎĽg |
Shelf Life | At least 1 year |
Elution Volume | Elution Volume: 100 - 200 ÎĽL |
Bacterial Endotoxins | <50 EU/ng DNA |
For expression of hROR alpha protein in mammalian cells, induction of G6Pase promoter activity
For expression of hROR gamma protein in mammalian cells, induction of IL-17A promoter activity
Vimentin-Chromobody™ fused to TagGFP2.
Nuclear Actin-Chromobody™ fused to TagGFP2.