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Thermo Scientific™ Rapid DNA Ligation Kit Product Code.: 10775841

Thermo Scientific™ Rapid DNA Ligation Kit

Product Code. p-4530525 Shop All Thermo Scientific Products
50 Reactions, 50 reactions
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Quantity:
150 Reactions
50 Reactions
Unit Size:
150 reactions
50 reactions
This item is not returnable. View return policy

Product Code. 10775841

Brand: Thermo Scientific™ K1422

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This item is not returnable. View return policy

Enable fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.

Rapid DNA Ligation Kit enables fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.

The kit contains T4 DNA ligase and a specially-formulated 5X rapid ligation buffer optimized for fast and efficient DNA ligation. Fast ligation efficiency is equal to that obtained with T4 DNA ligase in a standard 1 hour ligation. The ligation reaction mixture can be used directly for bacterial transformation with the TransformAid Bacterial Transformation Kit or with other conventional transformation procedures.

The kit contains T4 DNA ligase and a specially formulated 5X rapid ligation buffer optimized for fast and efficient DNA ligation. Fast ligation efficiency is equal to that obtained with T4 DNA ligase in a standard 1 hour ligation. The ligation reaction mixture can be used directly for bacterial transformation with the TransformAid Bacterial Transformation Kit or with other conventional transformation procedures.
  • Fast—ligation of either sticky- or blunt-end DNA in only 5 minutes
  • Convenient—reaction mixture can be used directly for bacterial transformation

Recommended for:

Routine cloning experiments; Blunt-end cloning; Library construction; TA cloning.

Note:

Lyophilized bacterial strains GM2163 (†M0099) and JM107 (†M0109) can be provided free of charge upon request, with any purchase. Prior to electroporation it is necessary to inactivate T4 DNA ligase (1) by chloroform extraction.

TRUSTED_SUSTAINABILITY

Specifications

Buffer 5X Rapid Ligation Buffer
Enzyme DNA Ligase
Product Type Rapid DNA Ligation Kit
Quantity 50 Reactions

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Lot Number Certificate Type Date Product Code
Lot Number2822089 Certificate TypeCertificate of Analysis Date29/01/2025 Product CodeK1422
Lot Number2825198 Certificate TypeCertificate of Analysis Date29/01/2025 Product CodeK1422
Lot Number2792572 Certificate TypeCertificate of Analysis Date29/01/2025 Product CodeK1422
Lot Number2841211 Certificate TypeCertificate of Analysis Date29/01/2025 Product CodeK1422
Lot Number2866370 Certificate TypeCertificate of Analysis Date28/01/2025 Product CodeK1422

For Research Use Only. Not for use in diagnostic procedures.

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