Alle primære antistoffer
Primære antistoffer, immunoglobuliner, der binder til specifikke proteiner eller andre biomolekyler, bruges i mange forskningsapplikationer og protokoller til at påvise mål af interesse. De er udviklet ved hjælp af forskellige dyreværter, herunder mus, rotte, kanin, ged, får og mange andre.
Monoklonale
og polyklonale antistoffer En type primære antistoffer, monoklonale antistoffer, giver høj reproducerbarhed og lav krydsreaktivitet og baggrundsstøj. En anden type, polyklonale antistoffer, koster ofte mindre og giver større affinitet og hurtigere binding. Begge er produceret ved hjælp af plasma B-celler, men førstnævnte bruger den samme klon, og sidstnævnte bruger forskellige kloner. Monoklonale antistoffer kræver hybridomcellelinjer, og polyklonale antistoffer gør det ikke.
Der er også rekombinante monoklonale antistoffer med lignende fordele, såsom høj affinitet, skalerbarhed og specificitet. De er produceret vha in vitro kloning af plasma B-celler og ekspressionsværter.
Konjugerede primære
antistoffer Antistoffer kan mærkes med forskellige fluoroforer eller detektionsmidler eller anvendes uden mærker. Mærkede primære antistoffer, også kendt som konjugerede primære antistoffer, hjælper forskere med at forenkle og strømline deres applikationer. De er koblet med almindelige enzymer og farvestoffer såsom Alexa Fluor og bruges ofte i protein- og celleanalyse.
Anvendelser Antistoffer til biovidenskabsapplikationer bruges i flowcytometri, western blotting, ELISA, immunhistokemi og immuncytokemi. Sekundære antistoffer kan tilsættes for at understøtte påvisning og oprensning af visse antigener. De binder til det primære antistof, som binder til antigenet af interesse. At finde den rigtige kombination af antistoffer kan resultere i større antigenspecificitet og et stærkt, detekterbart signal.
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Filtrerede søgeresultater
| Værtsarter | Rabbit |
|---|---|
| Form | Purified |
| Konjugeret | Unconjugated |
| Isotype | IgG |
| Koncentration | 1 mg/mL |
| Fortynding | Western Blot 1-3 ug/ml, Immunohistochemistry-Paraffin 10 ug/ml |
| Primær eller sekundær | Primary |
| Genadgangsnr. | Q96RJ0 |
| Klassifikation | Polyclonal |
| Antigen | Trace Amine Receptor 1 |
| Gene Alias | MGC126874, MGC138399, TA1RP11-295F4.9, TaR-1, TAR1trace amine-associated receptor 1, trace amine associated receptor 1, Trace amine receptor 1taR-1, TRAR1 |
| Gensymboler | TAAR1 |
| Applikationer | Western Blot,Immunohistochemistry (Paraffin) |
| Indhold og opbevaring | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Immunogen | A portion of amino acids 225-250 of human TAAR1 was used as the immunogen |
| Formulering | 0.2 ml PBS and 0.05% BSA with 0.05% Sodium Azide |
| Målarter | Human,Primate |
| Regulatorisk status | RUO |
| Gen-id (Entrez) | 134864 |
| Testspecificitet | The amino acid sequence used as immunogen is 100% homologous in human and chimpanzee, 86% homologous in mouse and 80% homologous in dog. |
| Oprensningsmetode | Protein G purified |
| Forskningsdisciplin | GPCR |
| Værtsarter | Rabbit |
|---|---|
| Konjugeret | Unconjugated |
| Isotype | IgG |
| Fortynding | Immunohistochemistry, Immunohistochemistry-Paraffin 1:200 - 1:500 |
| Primær eller sekundær | Primary |
| Genadgangsnr. | Q96RJ0 |
| Klassifikation | Polyclonal |
| Antigen | Trace Amine Receptor 1 |
| Gene Alias | MGC126874, MGC138399, TA1RP11-295F4.9, TaR-1, TAR1trace amine-associated receptor 1, trace amine associated receptor 1, Trace amine receptor 1taR-1, TRAR1 |
| Gensymboler | TAAR1 |
| Applikationer | Immunohistochemistry,Immunohistochemistry (Paraffin) |
| Immunogen | This antibody was developed against a recombinant protein corresponding to amino acids: KEQARLISDANQKLQIGLEMKNGISQSKER |
| Formulering | PBS (pH 7.2) and 40% Glycerol with 0.02% Sodium Azide |
| Målarter | Human |
| Regulatorisk status | RUO |
| Gen-id (Entrez) | 134864 |
| Testspecificitet | Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins. |
| Oprensningsmetode | Affinity Purified |
| Forskningsdisciplin | GPCR |
Trace Amine Receptor 1 Antibody, Novus Biologicals™
Rabbit Polyclonal Antibody has been used in 1 publication
| Værtsarter | Rabbit |
|---|---|
| Form | Purified |
| Konjugeret | Unconjugated |
| Isotype | IgG |
| Koncentration | 1.0 mg/mL |
| Fortynding | Western Blot 1-3 ug/ml, Immunohistochemistry-Paraffin |
| Primær eller sekundær | Primary |
| Genadgangsnr. | Q96RJ0 |
| Klassifikation | Polyclonal |
| Antigen | Trace Amine Receptor 1 |
| Gene Alias | MGC126874, MGC138399, TA1RP11-295F4.9, TaR-1, TAR1trace amine-associated receptor 1, trace amine associated receptor 1, Trace amine receptor 1taR-1, TRAR1 |
| Gensymboler | TAAR1 |
| Applikationer | Western Blot,Immunohistochemistry (Paraffin) |
| Indhold og opbevaring | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Immunogen | A portion of amino acids 200-230 of human TAAR1 was used as the immunogen |
| Formulering | PBS with 0.05% Sodium Azide |
| Målarter | Human,Mouse,Rat,Primate |
| Regulatorisk status | RUO |
| Gen-id (Entrez) | 134864 |
| Testspecificitet | The amino acid sequence used as immunogen is 100% homologous in human and chimpanzee, 93% homologous in rhesus monkey, 86% homologous in dog and 50% homologous in mouse and rat. |
| Oprensningsmetode | Protein G purified |
| Forskningsdisciplin | GPCR |
Invitrogen™ TAAR6 Polyclonal Antibody
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Rabbit Polyclonal Antibody
Invitrogen™ TAAR8 Polyclonal Antibody
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Rabbit Polyclonal Antibody
Invitrogen™ TAAR1 Polyclonal Antibody
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Rabbit Polyclonal Antibody
Invitrogen™ TAAR8 Polyclonal Antibody
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Rabbit Polyclonal Antibody
Invitrogen™ TAAR6 Polyclonal Antibody
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Rabbit Polyclonal Antibody
Invitrogen™ TAAR6 Polyclonal Antibody
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Rabbit Polyclonal Antibody
Invitrogen™ TAAR6 Polyclonal Antibody
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Rabbit Polyclonal Antibody