Buffere og standarder
Hydrogenionbuffere omfattende en blanding af en svag syre og dens konjugatbase eller omvendt og anvendt til at stabilisere pH; også fortyndingsmidler, vaskeopløsninger og standardværdiopløsninger, der anvendes til en bred vifte af videnskabelige kalibreringsformål.
De buffere, der bruges til at kalibrere pH-målere, kan være certificeret og/eller sporbare til National Institute of Standards and Technology (NIST). Disse buffere kan også være farvekodede for nem identifikation:
- Rød: pH 4,0 Gul:
- pH 7,0 Blå:
- pH 10,0
Filtrerede søgeresultater
TE-buffer, Tris-EDTA, 1X opløsning, pH 7,4, molekylærbiologi, Fisher BioReagents™
Tris-EDTA (TE) bruges rutinemæssigt til at suspendere nukleinsyreprøver.
| Kemisk navn eller materiale | Tris-EDTA |
|---|---|
| Sundhedsfare 3 | May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. |
| Sundhedsfare 2 | CAUTION! |
| DNase | DNase free |
| Fysisk form | Liquid |
| Farve | Colorless |
| Grad | Molecular Biology |
| Navn note | 1X Solution, pH 7.4 |
| Anbefalet opbevaring | RT |
| CAS | 7732-18-5 |
| Protease | Protease free |
| Gennemfiltreret | Filtered through a 5-micron filter. |
| pH | 7.4 to 8.1 |
| Synonym | TE |
| ChemAlert Opbevaringssymbol | Gray |
TE Buffer, 1X Solution, Molekylær Biology Grade, Ultrapure , Thermo Scientific Chemicals
CAS: 1185-53-1 Molekylær formel: C4H12ClNO3 Molekylvægt (g/mol): 157.594 InChI nøgle: QKNYBSVHEMOAJP-UHFFFAOYSA-N PubChem CID: 93573 IUPAC navn: 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride SMIL: C(C(CO)(CO)N)O.Cl
| PubChem CID | 93573 |
|---|---|
| Molekylvægt (g/mol) | 157.594 |
| CAS | 1185-53-1 |
| SMIL | C(C(CO)(CO)N)O.Cl |
| IUPAC navn | 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride |
| InChI nøgle | QKNYBSVHEMOAJP-UHFFFAOYSA-N |
| Molekylær formel | C4H12ClNO3 |
TBS, 20X Solution, pH 7.4, Molecular Biology Grade, Ultrapure
CAS: 7647-14-5 Molekylær formel: ClNa Molekylvægt (g/mol): 58.44 MDL nummer: MFCD00003477 InChI nøgle: FAPWRFPIFSIZLT-UHFFFAOYSA-M PubChem CID: 5234 ChEBI: CHEBI:26710 IUPAC navn: sodium;chloride SMIL: [Na+].[Cl-]
| MDL nummer | MFCD00003477 |
|---|---|
| PubChem CID | 5234 |
| Molekylvægt (g/mol) | 58.44 |
| CAS | 7647-14-5 |
| ChEBI | CHEBI:26710 |
| SMIL | [Na+].[Cl-] |
| IUPAC navn | sodium;chloride |
| InChI nøgle | FAPWRFPIFSIZLT-UHFFFAOYSA-M |
| Molekylær formel | ClNa |
| Navn note | 1X, pH 8.0 |
|---|---|
| Kemisk navn eller materiale | Sodium Chloride-Tris-EDTA Buffer |
| Anbefalet opbevaring | RT |
| CAS | 7732-18-5 |
| Protease | Protease free |
| Gennemfiltreret | Filtered through a 5-micron filter. |
| pH | 8 |
| DNase | DNase free |
| Kogepunkt | 100°C |
| Fysisk form | Liquid |
| Farve | Undesignated |
| ChemAlert Opbevaringssymbol | Gray |
MOPS 10X Solution, Fisher BioReagents™
CAS: 1132-61-2 Molekylær formel: C7H15NO4S Molekylvægt (g/mol): 209.26 MDL nummer: MFCD00006183 InChI nøgle: DVLFYONBTKHTER-UHFFFAOYSA-N Synonym: 3-morpholinopropane-1-sulfonic acid,morpholinopropanesulfonic acid,morpholinopropane sulfonic acid,4-morpholinepropane sulfonic acid,unii-273bp63nv3,3-morpholinopropanesulfonic acid,3-n-morpholinopropanesulfonic acid,3-n-morpholino propanesulfonic acid,4-morpholinepropanesulfonic acid,3-[N-Morpholino]propane sulfonic Acid PubChem CID: 70807 ChEBI: CHEBI:44115 IUPAC navn: 4-(3-sulfonatopropyl)morpholin-4-ium SMIL: [O-]S(=O)(=O)CCC[NH+]1CCOCC1
| MDL nummer | MFCD00006183 |
|---|---|
| PubChem CID | 70807 |
| Molekylvægt (g/mol) | 209.26 |
| CAS | 1132-61-2 |
| ChEBI | CHEBI:44115 |
| Synonym | 3-morpholinopropane-1-sulfonic acid,morpholinopropanesulfonic acid,morpholinopropane sulfonic acid,4-morpholinepropane sulfonic acid,unii-273bp63nv3,3-morpholinopropanesulfonic acid,3-n-morpholinopropanesulfonic acid,3-n-morpholino propanesulfonic acid,4-morpholinepropanesulfonic acid,3-[N-Morpholino]propane sulfonic Acid |
| SMIL | [O-]S(=O)(=O)CCC[NH+]1CCOCC1 |
| IUPAC navn | 4-(3-sulfonatopropyl)morpholin-4-ium |
| InChI nøgle | DVLFYONBTKHTER-UHFFFAOYSA-N |
| Molekylær formel | C7H15NO4S |
| Kemisk navn eller materiale | Tris-EDTA |
|---|---|
| Sundhedsfare 3 | Emergency Overview May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Remove from exposure, lie down. Move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. If not breathing, give artificial respiration. Obtain medical attention. NFPA Health:1 Flammability:0 Instability:0 |
| Sundhedsfare 2 | CAUTION! |
| DNase | DNase free |
| Fysisk form | Liquid |
| Farve | Colorless |
| Grad | Molecular Biology |
| Navn note | 1X Solution, pH 7.6 |
| Anbefalet opbevaring | RT |
| CAS | 7732-18-5 |
| Protease | Protease free |
| Gennemfiltreret | Filtered through a 5-micron filter. |
| pH | 7.4 to 8.1 |
| Synonym | TE |
| ChemAlert Opbevaringssymbol | Gray |
| Kemisk navn eller materiale | Tris-EDTA (TE), 10X Solution |
|---|---|
| Sundhedsfare 3 | Emergency Overview May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Remove from exposure, lie down. Move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. If not breathing, give artificial respiration. Obtain medical attention. NFPA Health:1 Flammability:0 Instability:0 |
| Sundhedsfare 2 | CAUTION! |
| DNase | DNase free |
| Fysisk form | Liquid |
| Farve | Colorless |
| Grad | Molecular Biology |
| Navn note | pH 7.4 |
| Anbefalet opbevaring | RT |
| CAS | 7732-18-5 |
| Protease | Protease free |
| Gennemfiltreret | Filtered through a 5-micron filter and autoclaved. |
| pH | 7.4 to 8.1 |
| Synonym | TE |
| ChemAlert Opbevaringssymbol | Gray |
Ethylenediamine Tetraacetate, DEPC-Treated Solution (0.5M, pH 8.0), Fisher BioReagents
CAS: 60-00-4 Molekylær formel: C10H16N2O8 Molekylvægt (g/mol): 292.24 MDL nummer: MFCD00003541 InChI nøgle: KCXVZYZYPLLWCC-UHFFFAOYSA-N Synonym: edta,edetic acid,ethylenediaminetetraacetic acid,edathamil,versene,endrate,havidote,titriplex,edta acid,sequestrol PubChem CID: 6049 ChEBI: CHEBI:42191 IUPAC navn: 2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid SMIL: OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
| MDL nummer | MFCD00003541 |
|---|---|
| PubChem CID | 6049 |
| Molekylvægt (g/mol) | 292.24 |
| CAS | 60-00-4 |
| ChEBI | CHEBI:42191 |
| Synonym | edta,edetic acid,ethylenediaminetetraacetic acid,edathamil,versene,endrate,havidote,titriplex,edta acid,sequestrol |
| SMIL | OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O |
| IUPAC navn | 2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid |
| InChI nøgle | KCXVZYZYPLLWCC-UHFFFAOYSA-N |
| Molekylær formel | C10H16N2O8 |
| Kemisk navn eller materiale | Tris-EDTA (TE), 10X Solution |
|---|---|
| Sundhedsfare 3 | Emergency Overview May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Remove from exposure, lie down. Move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. If not breathing, give artificial respiration. Obtain medical attention. NFPA Health:1 Flammability:0 Instability:0 |
| Sundhedsfare 2 | CAUTION! |
| DNase | DNase free |
| Fysisk form | Liquid |
| Farve | Colorless |
| Grad | Molecular Biology |
| Navn note | pH 7.6 |
| Anbefalet opbevaring | RT |
| CAS | 7732-18-5 |
| Protease | Protease free |
| Gennemfiltreret | Filtered through a 5-micron filter and autoclaved. |
| pH | 7.4 to 8.1 |
| Synonym | TE |
| ChemAlert Opbevaringssymbol | Gray |