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Optimer kloning med Thermo Scientific™ pBR322 DNA, et 4361 bp dobbeltstrenget lukket cirkulært medium kopi plasmid DNA.
A fast way to purify transfection-grade plasmid DNA using mini-format technology to reduce endotoxins from bacterial lysates. Complete kit includes mini spin columns for an ultra-fast workflow.
Inkluderer | NucleoSpin Plasmid Transfection-grade Columns, Collection Tubes, Buffers, RNase A |
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Elueringsvolumen | Elution Volume: 30 - 50 μL |
Effektivitet | Binding capacity: 35 μg |
Til brug med (udstyr) | Centrifuge, Vacuum manifold (e.g. NucleoVac 96) |
Endelig produkttype | Transfection-grade plasmid DNA |
Test tid | 14 min/6 preps |
Udbytte | 15 - 30 μg |
Isoleringsteknologi | Silica Membrane and Endotoxin-reduction Technology |
Prøvetype | Bacterial Culture (E. coli, High-copy Plasmids) |
Størrelse | < 15 kbp |
Bakterielle endotoksiner | <50 EU/ng DNA |
Format | Mini spinsøjle |
Indhold og opbevaring | Room temperature (18 - 25 °C) |
Holdbarhed | At least 1 year |
Prøvevolumen | Up to 5 mL |
Renhed | A260/A280: 1.80 - 1.85 |
Til brug med (applikation) | Rapid purification of plasmids for transfection |
Mammalian expression vector construct, pCMV/hRUNX1, containing coding sequence of human Runt-related transcription factor 1
For expression of hRUNX1 protein in mammalian cells, induction of FOXP3 promoter activity
A fast way to purify transfection-grade plasmid DNA using mini-format technology to reduce endotoxins from bacterial lysates. Full and Core kits utilize a 96-well plate format for an efficient high-throughput process.
Elueringsvolumen | Elution Volume: 100 - 200 μL |
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Effektivitet | Binding capacity: 20 μg |
Til brug med (udstyr) | Centrifuge, Vacuum manifold (e.g. NucleoVac 96) |
Endelig produkttype | Transfection-grade plasmid DNA |
Test tid | 45 min/plate |
Udbytte | 5 - 20 μg |
Isoleringsteknologi | Silica Membrane and Endotoxin-reduction Technology |
Prøvetype | Bacterial Culture (E. coli, High-copy Plasmids) |
Størrelse | < 15 kbp |
Bakterielle endotoksiner | <50 EU/ng DNA |
Format | 96-brønds plade |
Indhold og opbevaring | Room temperature (18 - 25 °C) |
Holdbarhed | At least 1 year |
Prøvevolumen | Up to 5 mL |
Renhed | A260/A280: 1.80 - 1.85 |
Til brug med (applikation) | Rapid purification of plasmids for transfection |
For expression of hROR alpha protein in mammalian cells, induction of G6Pase promoter activity
Nuklear Actin-Chromobody™ fusioneret til TagGFP2.
For expression of hROR gamma protein in mammalian cells, induction of IL-17A promoter activity
Celle Cycle-Chromobody™ fusioneret til TagRFP.