Learn More
Invitrogen™ PiPer™ Pyrophosphate or Phosphate Assay Kit
PiPer Pyrophosphate Assay Kit provides reagents for fluorogenic or colorimetric detection as low as 0.4 mÎĽM (fluorescence) or 2 ÎĽM (absorbance) inorganic pyrophosphate (PPi) while Phosphate Assay kits can detect down to 0.2 ÎĽM Pi in purified enzyme systems. Each kit has enough for 1000 labelings.
6150.00 DKK - 6360.00 DKK
Specifications
Color | Red |
---|---|
Content And Storage | Store in freezer -5°C to -30°C and protect from light. |
Detection Method | Colorimetric, Fluorescence |
For Use With (Equipment) | Spectrophotometer, Microplate Reader |
Dye Type | Amplex™ Red |
Product Code | Brand | ProductTypeSpecs | Price | Quantity & Availability | |||||
---|---|---|---|---|---|---|---|---|---|
Product Code | Brand | ProductTypeSpecs | Price | Quantity & Availability | |||||
10504053
|
Invitrogen™
P22061 |
6360.00 DKK
Each |
Please sign in to purchase this item. Need a web account? Register with us today! | ||||||
10378442
|
Invitrogen™
P22062 |
6150.00 DKK
Each |
Please sign in to purchase this item. Need a web account? Register with us today! | ||||||
Description
PiPer Pyrophosphate and Phosphate Assay Kit provides an ultrasensitive assay to detect the fluorescent product resorufin. These kits can be used to detect inorganic pyrophosphate (PPi) or phosphate (Pi) in a variety of samples or can monitor the kinetics of their release by a variety of enzymes, including DNA/RNA polymerases, adenylate cyclase, S-acetyl coenzyme A synthetase, ATPases, GTPases, 5′-nucleotidase, protein phosphatases, acid/alkaline phosphatases and phosphorylase kinase. As resorufin has a strong absorption, this assay can be performed either fluorometrically or colorimetrically.
Key Features:
- High Sensitivity: Can detect low levels of inorganic phosphate
- Number of labelings per kit: ~1,000 of 100 ÎĽL per assay volume
- Detection method: Colorimetric, Fluorescence
- Compatibility: Can be used with both microplate readers and spectrophotometers
- Excitation/emission maxima: 563/587 nm
- Versatility: Suitable for a wide range of applications including enzyme activity assays
- Quantitative Results: Provides accurate and reproducible results.
The PiPer Pyrophosphate Assay Kit provides a sensitive fluorogenic or colorimetric method for detecting as little as 0.4 ÎĽM (fluorescence) or 2 ÎĽM (absorbance) inorganic pyrophosphate in a 100 ÎĽL assay volume. The PiPer Phosphate Assay Kit detects as little as 0.2 ÎĽM Pi (fluorescence) in purified enzyme systems with a 100 ÎĽL assay volume.
Use the PiPer Pyrophosphate and Phosphate Assay Kit for applications such as: Anion Detection, Biochemical Toxicology Assays, Cell Analysis, Cell Metabolism, Cell Viability, Cellular Toxicology Assays, Drug Discovery and Development, Enzyme and Protein Activity Assays, Ionic Homeostasis and Signaling, Phosphatase Activity, Phosphatase Activity Assays, Phosphatase Biology, Target and Lead Identification and Validation.
The absorption and fluorescence of resorufin are pH dependent. There is a pKa at ~6.0 where the absorption maximum shifts to ~480 nm and the fluorescence quantum yield is markedly lower. Given Amplex™ Red reagent is unstable at high pH (>8.5) and is sensitive to light, we recommend this reaction be performed using the provided reaction buffer (at optimal pH ~7.5) for best results and reagents kept protected from light. All kits should follow user manual guidelines.
Order Info
Shipping Condition: Room temperature
Specifications
Red | |
Colorimetric, Fluorescence | |
Amplex™ Red | |
PiPer | |
Room Temperature |
Store in freezer -5°C to -30°C and protect from light. | |
Spectrophotometer, Microplate Reader | |
96-well plate | |
1000 Assays | |
563/587 nm |
For Research Use Only. Not for use in diagnostic procedures.
Your input is important to us. Please complete this form to provide feedback related to the content on this product.