Thermo Scientific™ CaptureSelect™ Biotin Anti-IgG-CH1 Conjugate

Description

CaptureSelect Biotin Anti-IgG-CH1 Conjugate consists of a 14 kDa recombinant single domain antibody fragment (V_HH affinity ligand) that specifically binds to the CH1 domain of all four human IgG subclasses. The affinity ligand is chemically conjugated to biotin via an appropriate spacer that retains the binding reactivity of the ligand when used in combination with streptavidin-based conjugates or streptavidin pre-coated surfaces.

The CaptureSelect Biotin Anti-IgG-CH1 Conjugate allows you to

• Detect, quantitate, and characterize all human IgG antibodies (subclasses 1 to 4) and Fab fragments thereof, independent of light chain type, and total IgG in human plasma and/or serum samples
• Avoid cross-binding with other isotypes, such as IgA, IgM, IgD, and IgE, free light chains, or IgG from mouse sources or bovine sources such as FCS
• Screen antibody-antigen interactions—biotin anti-IgG-CH1 forms a stable [low k(diss)] complex with human IgG antibodies through bivalent binding

Applications for CaptureSelect Biotin Anti-IgG-CH1 Conjugate include ELISA, Western blot, Gyrolab™-based immunoassays, and label-free detection platforms such as those based on surface plasmon resonance (Biacore™ and IBIS-MX96 systems) and bio-layer interferometry (ForteBio™ Octet™ systems).

Animal origin statement

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (V_HH) created by a proprietary technology. The V_HH affinity ligand is a 12–15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).