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Invitrogen™ RETROscript™ Reverse Transcription Kit

Includes reagents for reverse transcription of RNA into double-stranded cDNA products

Brand:  Invitrogen™ AM1710

Product Code. 10585595

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Includes:
  • 50ÎĽL Control Template RNA (0.5 mg/ml)
  • 200ÎĽL 10X RT Buffer
  • 400ÎĽL 10X PCR Buffer
  • 300ÎĽL dNTP mix (2.5 mM each dNTP)
  • 80ÎĽL Random Decamers (50 ÎĽM)
  • 80ÎĽL Oligo(dT) Primers (50ÎĽL)
  • 40ÎĽL MMLV-RT (100 units/ÎĽL)
  • 40ÎĽL RNase Inhibitor (10 units/ÎĽL)
  • 50ÎĽL Control PCR Primers (5 ÎĽM each)
  • 1.0mL High Resolution Gel Loading Solution (5X)
  • 3.5mL Nuclease-free Water. 40 reactions.
This item is not returnable. View return policy

Description

Description

  • Amplify specific RNA targets from complex mixtures (i.e., total cellular RNA)
  • Both random decamers and oligo(dT)18 primers provided
  • Perform either one- or two-step RT-PCR
  • Optimal reaction temperature: 44° to 55°C
  • Final product size: 7kb or less

Accessory products: SuperTaq Polymerase (Mfr. Nos. AM2054 and AM2056) and SuperTaq Plus Polymerase (Mfr. Nos. AM2054 and AM2056) are recommended for use with the RETROscript Kit. RETROscript Kit components that are available separately include dNTPS for cDNA Probe Synthesis (Mfr. No. AM8200), Oligo (dT) Primer (Mfr. No. AM5730G), and Random Decamers (Mfr. No. AM5722G). Also available are the T7-Oligo (dT)24 Promoter Primer (Mfr. No. AM5710) and the T7-Oligo (dT)24 Promoter Primer, Anchored (Mfr. No. AM5712).

PCR & Real-Time PCR, Reverse Transcription

Order Info

Shipping Condition: Dry ice

TRUSTED_SUSTAINABILITY
Specifications

Specifications

50ÎĽL Control Template RNA (0.5mg/mL); 200ÎĽL 10X RT Buffer; 400ÎĽL 10X PCR Buffer; 300ÎĽL dNTP mix (2.5mM each dNTP); 80ÎĽL Random Decamers (50ÎĽM); 80ÎĽL Oligo(dT) Primers (50ÎĽL); 40ÎĽL MMLV-RT (100 units/ÎĽL); 40ÎĽL RNase Inhibitor (10 units/ÎĽL); 50ÎĽL Control PCR Primers (5ÎĽM each); 1.0mL High Resolution Gel Loading Solution (5X); 3.5mL Nuclease-free Water. Store Control Template RNA below -70deg.C. Store all other reagents at -20deg.C.
40 reactions
RNA
Separate Components
cDNA Synthesis, RT-PCR
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Product Information

Manuals

Protocol: RETROscript® Kit (English ) Version: 09 Sep 2010 File size: 432.1 kB
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For Research Use Only. Not for use in diagnostic procedures.