Modifying Enzymes
Modifying Enzymes
Proteins that have the ability to structurally alter macromolecules without being altered themselves. Useful for a variety of molecular biology procedures; products include DNA and RNA polymerases, phosphatases, kinases, nucleases, etc.
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brands:
Fisher Bioreagents
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Content And Storage | -20°C |
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Form | Solid |
Product Type | Proteinase K |
Color | White |
For Use With (Application) | Isolation of DNA and RNA from cell and tissue preparations, inactivating many Enzymes, including Endogenous Nucleases |
Source | Tritirachium album |
Content And Storage | -20°C |
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Form | Liquid |
Product Type | T4 DNA Polymerase |
Storage Buffer | OPTIZYME™ T4 DNA Pol Buffer 5X: Tris HCl 335mM (8.8 pH at 25°C), MgCl2 33mM, DTT 5mM, (NH4)2SO4 84mM |
Color | Colorless |
pH | 7.5 |
Concentration | 5 U/μL |
For Use With (Application) | Synthesis of labeled DNA probes, creation of blunt PCR products with 3'-dA overhangs, ligation-independent cloning of PCR products, Restriction Enzymes, reverse transcriptases and T4 DNA ligase |
Source | E. coli cells with a cloned gene 43 of bacteriophage T4 |
Fisher BioReagents™ Optizyme™ Ribonuclease Inhibitor, Recombinant
Protects RNA from degradation by RNases
Content And Storage | <0°C |
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Form | Crystalline Powder |
Product Type | Lysozyme |
Color | White |
For Use With (Application) | Used to lyse E. coli for preparation of plasmid DNA from transformed cell cultures |
Source | Egg white |
Content And Storage | -20°C |
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Form | Liquid |
Product Type | DNA Polymerase I |
Storage Buffer | OPTIZYME™ DNA Pol I Buffer 10X : Tris HCl 500mM (7.5 pH at 25°C), MgCl2 100mM, DTT 10mM |
Color | Colorless |
pH | 7.5 |
Concentration | 10 U/μL |
For Use With (Application) | DNA Polymerase I labels DNA by nick translation in conjunction with DNase I, second-strand cDNA synthesis in conjunction with RNase H, incorporation of modified Nucleotides, Reverse Transcriptases |
Source | E. coli cells with a cloned polA gene |
Content And Storage | -20°C |
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Form | Liquid |
Product Type | OPTIZYME™ M-MLV Reverse Transcriptase |
Storage Buffer | OPTIZYME™ M-MLV RT Buffer 5X : Tris HCl 250mM (8.3 pH at 25°C), KCl 250mM, MgCl2 20mM, DTT 50mM |
Color | Colorless |
pH | 8.3 |
Concentration | 200 U/μL |
For Use With (Application) | Generation of labeled cDNA probes for microarrays, synthesis of cDNA for cloning and expression, analysis of RNA by primer extension, DNA labeling, optimum activity at 42°C, incorporates modified Nucleotides |
Source | E. coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus Reverse Transcriptase |
Content And Storage | -20°C |
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Form | Chunks, Powder |
Product Type | Leupeptin Hemisulfate |
Molecular Weight (g/mol) | 475.60 |
Color | White |
For Use With (Application) | Inhibitor for Trypsin, Plasmin, Papain, Cathepsin B |
Content And Storage | -20°C |
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Form | Liquid |
Product Type | T4 DNA Ligase |
Storage Buffer | OPTIZYME™ T4 DNA Ligase Buffer 10X: Tris HCl 400mM, MgCl2 100mM, DTT 100mM, ATP 5mM (7.8 pH at 25°C), OPTIZYME™ PEG 4000 Solution 50%: Polyethylene Glycol 50% (w/v) |
Color | Colorless |
pH | 7.5 |
Concentration | 5 U/μL |
For Use With (Application) | Used to clone restriction Enzyme generated DNA fragments, cloning PCR products, ligation of double-stranded Oligonucleotide linkers or adaptors to DNA, site-directed mutagenesis, amplified fragment length polymorphism, ligase-mediated RNA detection, nick |
Source | E. coli cells with a cloned gene 30 of bacteriophage T4 |
Content And Storage | -20°C |
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Form | Solid |
Product Type | Alkaline Phosphatase |
Storage Buffer | OPTIZYME™ AP Buffer 10X : Tris HCl 100mM (8 pH at 37°C), MgCl2 50mM, KCl 1M, Triton™ X-100 0.2%, BSA 1mg/mL |
Color | Colorless |
pH | 7.4 |
Concentration | 1 U/μL |
For Use With (Application) | DePhosphorylates cloning vector DNA (10 min. at 37°C), fast thermo-inactivation (5 min. at 75°C), Nucleotide degradation prior to sequencing PCR products, active on Nucleotides, Protein dephosphorylation, restriction En |
Source | E. coli cells with a cloned bacterial Alkaline Phosphatase gene |