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Beskrivelse
- Strep-Tactin™XT Spin Column Kit contains all essential reagents required for purification of Strep-tag™II and Twin-Strep-tag™ fusion proteins from small samples.
- It provides Buffer W, Buffer BXT, 25 spin columns and receiver tubes.
- Spin columns are filled with dried polymethacrylate, a synthetic material coupled with the streptavidin variant Strep-Tactin™XT and are compatible with conventional microcentrifuges, allowing a rapid, parallel processing of multiple samples.
- With one column up to 400 μg of mCherry-Strep-tag™II (28 kDa) can be purified under physiological conditions in less than 10 minutes.
- Prior to application, the spin columns need to be re-hydrated and activated with Buffer W.
- In contrast to agarose resins, plant cell components such as chlorophyll are not unspecifically bound by the resin, making the Strep-Tactin™XT Spin Column Kit a favorable alternative.
- Please note that the spin columns are not reusable.
Tekniske data
Tekniske data
| Sættets indhold | Spin columns, reviever tubes, wash and elution buffer, purification protocol |
| Mængde | 1 Kit |
For Research Use Only.
Produkttitel
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