IBA Lifesciences

IBA Lifesciences provides high performance research tools for cell and protein isolation based on their proprietary Strep-tag™ technology. Their product portfolio offers solutions for the entire production chain of recombinant proteins, from cloning, transfection, protein expression and purification to detection, immobilization and assays, as well as innovative cell isolation and expansion tools.

IBA's Cell Selection and Expansion

TACS - Traceless Affinity Cell Selection

Fab-TACS™ technology perfectly combines the benefits of positive and negative cell selection:

High purity, yield and viability
Label-free, non-activated cells due to fully reversible binding
From whole blood, buffy coat, PBMCs or murine samples
Affinity chromatographic, magnetic or fluorescent cell separation

TACS - Traceless Affinity Cell Selection

MHC I Streptamer™

Detection and isolation of antigen-specific T-cells with MHC I Streptamers™:

Label-free cell isolation according to antigen specificity
High specificity, sensitivity, and reproducibility
From single cell suspensions (e.g., PBMCs or murine samples)
Affinity chromatographic, magnetic or fluorescent cell separation

Cell Stimulation and Expansion

CD3: CD28 Streptamers™:

Completely reversible reagents
Termination of stimulation at any given point in time
Adjustable CD3: CD28 ratio
Bead-free

Peptides for T-Cell Stimulation:

Purity >85%
Endotoxin-free

Fluorescent Cell Staining

Detection of antigen-specific T-cells with MHC I Streptamers™ and of receptor-specific T-cells with Fab Streptamers™:

Fully reversible
High specificity, sensitivity, and reproducibility
No leftover staining reagents on the cells after FACS sorting
Optimal preservation of cell effector function
Improved viability of cells

Protein Production and Assay

Cloning

More than 150 Twin-Strep-tag™/Strep-tag™II expression vectors:

Variety of Twin-Strep-tag™/Strep-tag™ II combined with His, FLAG or GST
Different N-/C-terminal orientations
Expression hosts: E. coli, mammalia, insect cells, yeast
Easy cloning via StarGate vectors

Mammalian Expression

The MEXi mammalian expression system is optimised for efficient protein expression and Strep-tag™ purification in mammalian cells:

Perfectly matched HEK293E cell line and cell culture media
Transient expression for time and cost efficient protein production
Multiple expression vectors with different features

Protein Purification

Strep-tag™ technology from the original manufacturer:

High affinity in low pM range
Rapid one-step purification with unparalleled protein purity (>95%)
Mild and specific elution
Tolerates broad range of buffer conditions
Suitable for metalloproteins, membrane proteins, low abundant proteins, and protein complexes amongst others

Detection

For Western blot analysis, immunofluorescence, FACS and ELISA.

Two different detection systems are provided:

Strep-Tactin™ and Strep-Tactin™ XT conjugate for Twin-Strep-tag™/Strep-tag™ II proteins
Monoclonal antibodies against Twin-Strep-tag™/Strep-tag™ II

Immobilization

With near covalent binding affinity, – Immobilisation by Strep-Tactin XT: Twin-Strep-tag can be used for screenings, diagnostic assays, protein biochemistry, protein kinetics and interaction studies, surface plasmon resonance (SPR) experiments (Biacore) or bio-layer interferometry (BLI):

High affinity in low pM range enables measurements over a long time
Minimal non-specific binding
Minimal coefficients of variation (cv)
Chip for SPR experiments can be easily regenerated

Surface-Marker Specific Exosome Isolation

Quick and simple isolation of unlabeled exosomes using Fab-TACS™ for exosomes, which offers:

Highly specific and reproducible isolation
Minimal contamination by other EVs
Label-free exosomes with intact biological functions
Source-independent application
Reversible capture and release of exosomes
A quick and simple protocol, without magnetic beads or ultracentrifugation

Surface-marker Specific Exosome Isolation

Immobilization with Near Covalent Binding Affinity

Reversible capture and analysis of Twin-Strep-tag™ fusion proteins for bio-layer interferometry (BLI) or surface plasmon resonance (SPR) experiments:

Indirect immobilization of the ligand prevents it from being subject to inactivation or influences of the immobilization procedure
High specificity and affinity in low pM range of the Twin-Strep-tag™ - Strep-Tactin™ XT interaction
Ligands can be captured directly from complex samples
Strep-Tactin™ XT biosensors/chips can be easily regenerated by an established procedure, whereby further ligand-analyte interactions can be analyzed with the same biosensor/chip

Immobilization with Near Covalent Binding Affinity

Our Perfect Tool for Isolation of Proteins or Complexes

MagStrep 'Type 3' XT Magnetic Beads offer:

High specificity and low background resulting in high protein purity
High binding affinity and capacity for Strep-tag™ II or Twin-Strep-tag™ fusion proteins
Immunoprecipitation (IP) or co-immunoprecipitation (Co-IP) in batch format and/or high throughput screening for protein purification via pull-downs
Suitable for usage with (semi-)automated magnetic bead purification systems (e.g., Thermo Scientific™ KingFisher™) for automated workflows

Our Perfect Tool for Isolation of Proteins or Complexes

Efficient Purification Even of Large Proteins

The Strep-Tactin™ XT 4Flow™ Starter Kit consists of everything you need for a quick start:

Strep-Tactin™ XT 4Flow™ gravity flow column
All buffers required for washing, elution, and regeneration
Strep-Tactin™ conjugated with horseradish peroxidase (HRP; for Western blot detection)
Short protocol

Efficient Purification Even of Large Proteins

Strep-Tactin™ for Reversible Binding of Biotinylated Proteins

Applicable for purification, detection, and immobilization of biotinylated molecules:

Suitable for proximity-dependent biotin identification (BioID) experiments
Mild and highly specific elution facilitating analysis of protein complexes
Strep-Tactin™ Sepharose™ and Strep-Tactin™ Superflow™ for gravity flow column-based affinity purification
MagStrep 'Type 3' Strep-Tactin™ magnetic beads are suitable for batch purification as well as pull-down assays

Strep-Tactin™ for Reversible Binding of Biotinylated Proteins

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IBA Lifesciences Desthiobiotin

Desthiobiotin pulver til fremstilling af Strep-Tactin™ elueringsbuffer

Pris og tilgængelighed
Tekniske data

Kemisk navn eller materiale	Desthiobiotin
Til brug med (applikation)	Til eluering af Strep-Tag II-proteiner fra Strep-Tactin

IBA Lifesciences Strep-Tactin ™XT 4Flow™ High Capacity Spin Column Kit

Strep-™Tactin XT 4Flow™ høj kapacitet Spin Column Kit til hurtig oprensning af Strep-tag ™II og Twin-Strep-tag™ fusionsproteiner.

Pris og tilgængelighed

IBA Lifesciences Strep-Tactin ™XT 4Flow™ Harpiks

Strep-Tactin ™XT 4Flow™ harpiks er en 50% suspension til at rense Strep-™tag II eller Twin-Strep-tag™ fusionsproteiner

Pris og tilgængelighed

IBA Lifesciences Biotin

Pris og tilgængelighed

IBA Lifesciences pASK-IBA E. coli vektor

pASK-IBA E. coli-ekspressionsvektorer, der koder for en Strep-tag ™II og chloramphenicol-resistens

Pris og tilgængelighed
Tekniske data

Format	Affjedring
Opbevaringsbuffer	TE buffer
Antibiotikaresistens bakteriel	Kloramfenicol
Replikationsoprindelse	F1 og ColE1 oprindelse
Udtrykssystem	E. coli
Kloningsmetode	Direkte kloning
Størrelsesområde	ca. 3 kb
Promotor	Tet
Koncentration	250 ng/μ L
Protein tag	Strep-tag II

IBA Lifesciences Tyngdekraft flow Strep-Tactin™ 4Flow™ høj kapacitet kolonne

Strep-Tactin™ 4Flow™ høj kapacitet tyngdekraft flow kolonne til at rense Strep-™tag II eller Twin-Strep-tag™ fusionsproteiner

Pris og tilgængelighed
Tekniske data

Format	Gravity flow kolonne
Protein form	Tetramerisk protein
Stationær fase	Agarose
Endelig produkttype	Affinitetskromatografiudstyr
Mål	Proteiner mærket med Twin-Strep-tag™ ; Strep-™tag II; krydsreaktivitet: biotin
Form	Harpiksopslæmning
Partikelstørrelse	50 til 150μ m
Bindende Kapacitet	20 mg/ml harpiks
Oversigt	4% agarose, tværbundet
Ligand type	Twin-Strep-tag™ ; Strep-™tag II; krydsreaktivitet: biotin
Kolonnetype	Forpakket gravitationsstrømsøjle

IBA Lifesciences Strep-Tactin™ 4Flow™ høj kapacitet 50% affjedring

Strep-Tactin™ 4Flow™ høj kapacitet 50% Suspension til at rense Strep-™tag II el Twin-Strep-tag™ fusionsproteiner

Pris og tilgængelighed
Tekniske data

Type	Bulk harpiks
Format	Flaske
Protein form	Tetramerisk protein
Stationær fase	Agarose
Endelig produkttype	Affinitetskromatografiudstyr
Mål	Proteiner mærket med Twin-Strep-tag™ ; Strep-™tag II; krydsreaktivitet: biotin
Form	Harpiksopslæmning
Partikelstørrelse	50 til 150μ m
Bindende Kapacitet	20 mg/ml harpiks
Oversigt	4% agarose, tværbundet
Ligand type	Twin-Strep-tag™ ; Strep-™tag II; krydsreaktivitet: biotin
Kolonnetype	Affjedring
Koncentration	50% suspension

IBA Lifesciences 10x Buffer BXT

IBA Lifesciences GmbH 10x Buffer BXT; Strep-Tactin ™XT Elution B. er en klar til brug buffer til eluering af Strep-mærkede proteiner fra Strep-Tactin™ XT.

Pris og tilgængelighed

IBA Lifesciences MagStrep Strep-Tactin™ XT perler

MagStrep Strep-Tactin™ XT perler er magnetiske perler (5% suspension) til at rense Strep-tag™ II eller Twin-Strep-tag™ fusionsproteiner.

Pris og tilgængelighed
Tekniske data

Type	Magnetisk agarose perle
Mål	Twin-Strep-tag eller Strep-tag II
Form	Harpiksopslæmning
Partikelstørrelse	20 til 40μ m
Formulering	5 % (v/v) suspension i PBS med 0,1 % (v/v) natriumazid
Support Type	6 % agarose, tværbundet

IBA Lifesciences BioLock

BioLock er en biotinblokerende opløsning til lysater og kultursupernatanter

Pris og tilgængelighed
Tekniske data

Type	Blokerende løsning

IBA Lifesciences 10x Buffer W - Vaskebuffer

Strep-Tactin™ og Strep-™Tactin XT vaskebuffer

Pris og tilgængelighed

IBA Lifesciences Strep-Tactin ™XT 4Flow™ Høj kapacitet patron

Strep-™Tactin XT 4Flow™ højkapacitets patron er en HPLC/FPLC patron til at rense Strep-tag ™II eller Twin-Strep-tag™ fusionsproteiner

Pris og tilgængelighed
Tekniske data

Format	Færdigpakket patron
Mål	Twin-Strep-Tag™ og Strep-tag ™II
Partikelstørrelse	50 til 150μ m
Support Type	4% agarose, tværbundet
Til brug med (applikation)	HPLC/FPLC

IBA Lifesciences Strep-Tactin ™XT konjugat

Strep-Tactin ™XT konjugerer med fluorescerende mærke for at detektere Strep-™tag II og Twin-Strep-tag™ fusionsproteiner

Pris og tilgængelighed
Tekniske data

Sættets indhold	Opløsning i PBS; 0,01 % NaN3; 1 mg/ml BSA
Type	Konjugeret
Mængde	50μ g
Kapacitet (metrisk)	0.10 mL
Mål	Strep-tag ™II og Twin-Strep-tag™
Indhold og opbevaring	Ufortyndet: 2 °C til 8 °C, beskyttes mod lys; Fortyndet: -25 °C til -15 °C
Koncentration	0.5 mg/mL

IBA Lifesciences Strep-Tactin™ protein som frysetørret pulver

Strep-Tactin™ protein som lyofiliseret pulver kan bruges til reversibel immobilisering af biotinylerede analytter eller Strep-tag ™II og Twin-Strep-tag™ fusionsproteiner

Pris og tilgængelighed
Tekniske data

Bindende Kapacitet	>50 nmol/mg

IBA Lifesciences GFP-Strep-tag control protein

GFP-Strep-tag control protein for Strep-Tactin™ and Strep-Tactin™XT applications

Pris og tilgængelighed
Tekniske data

Inkluderer	500 μg of a 30 kDa protein

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Cell Selection and Expansion

Application Note Central memory T-cells isolated and stained with CD8α and CD62L Fab Streptamer reagents

Filename: ApplicationNote-Central-memory-T-cells-isolated-and-stained-with-CD8α-and-CD62L-Fab-Streptamer-reagents.pdf
Size: 662 KB
Format: application/pdf

Application Note-Combining-MHC-I-and-CD8+-Fab-Streptamer-reagents

Filename: Application_Note_Combining.pdf
Size: 803 KB
Format: application/pdf

Application Note Regulatory T-cells isolated and stained with CD4-CD25 and CD45RA Fab Streptamer reagents

Filename: ApplicationNote-Regulatory-T-cells-isolated-and-stained-with-CD4-CD25-and-CD45RA-Fab-Streptamer-reagents.pdf
Size: 737 KB
Format: application/pdf