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Beskrivelse
Strep-Tactin™XT Buffer Set contains all buffers necessary for purification of Strep-tag™II or Twin-Strep-tag™ fusion proteins with Strep-Tactin™XT as well as for subsequent regeneration of the resin. 10x Buffer W is a 10x concentrated wash buffer for Strep-Tactin™ and Strep-Tactin™XT resins. It removes unbound molecules from the affinity chromatography matrix during Strep-tag™II and Twin-Strep-tag™ fusion protein purification. 10x Buffer BXT is a 10x concentrated Strep-Tactin™XT elution buffer containing biotin. Biotin is a water-soluble B-complex vitamin, also known as vitamin B7 or vitamin H. It binds to Strep-Tactin™XT with very high affinity but reversibly and, thereby, allows the efficient elution of Strep-tag™II or Twin-Strep-tag™ fusion proteins from the resin. Please note that 10x Buffer BXT is not applicable for elution of Strep-tag™II or Twin-Strep-tag™ fusion proteins from Strep-Tactin™, since biotin irreversibly binds to these resins, and, thus, Strep-Tactin™ cannot be regenerated. Buffer XT-R is a ready-to-use Strep-Tactin™XT regeneration buffer containing magnesium chloride for competitive displacement of biotin from the modified biotin-binding pocket of Strep-Tactin™XT after the elution of Strep-tag™II or Twin-Strep-tag™ fusion proteins. Please note that the Strep-Tactin™XT Buffer Set is not applicable for Strep-Tactin™.
Tekniske data
Tekniske data
| Koncentration | 10 x concentrated |
| Sættets indhold | 100 mL 10 x Buffer W, 25 mL 10 x Buffer BXT, 250 mL Buffer XT-R |
| Forsendelsestilstand | room temperature |
| Form | Reagensopløsning |
| Mængde | 1 sæt |
Sikkerhed og håndtering
Produktidentifikator
- 1SET Strep-TactinXT Purification Buffer Set
- Advarsel
- Alvorlige øjenskader/øjenirritation Kategori 2
- Hudætsning/hudirritation Kategori 2
- H315-Forårsager hudirritation.
- H319-Forårsager alvorlig øjenirritation.
- P264-Vask grundigt efter brug.
- P280-Bær beskyttelseshandsker/beskyttelsestøj/øjenbeskyttelse/ ansigtsbeskyttelse.
- P302+P352-VED KONTAKT MED HUDEN: Vask med rigeligt vand/.
- P305+P351+P338-VED KONTAKT MED ØJNENE: Skyl forsigtigt med vand i flere minutter. Fjern eventuelle kontaktlinser, hvis dette kan gøres let. Fortsæt skylning.
- P332+P313-Ved hudirritation: Søg lægehjælp.
- P337+P313-Ved vedvarende øjenirritation: Søg lægehjælp.
Produkttitel
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