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  5. IBA Lifesciences StarGate pLSG-IBA Vectors for Baculo

IBA Lifesciences StarGate pLSG-IBA Vectors for Baculo

Artikelnummer. 15673828 Shop alle IBA Lifesciences produkter
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Vektor:
pLSG-IBA 102
pLSG-IBA 103
pLSG-IBA 104
pLSG-IBA 105
pLSG-IBA 123
pLSG-IBA 142
pLSG-IBA 143
pLSG-IBA 144
pLSG-IBA 145
pLSG-IBA 162
pLSG-IBA 164
pLSG-IBA 167
pLSG-IBA 168
pLSG-IBA 23
pLSG-IBA 25
pLSG-IBA 3
pLSG-IBA 33
pLSG-IBA 35
pLSG-IBA 43
pLSG-IBA 45
pLSG-IBA 5
pLSG-IBA 62
pLSG-IBA 63
pLSG-IBA 64
pLSG-IBA 65
pLSG-IBA wt1
pLSG-IBA wt2
Pakningsstørrelse:
5µg
27 product options available for selection
Product selection table with 27 available options. Use arrow keys to navigate and Enter or Space to select.
Artikelnummer. Vektor unitSize
15673828 pLSG-IBA 25 5µg
15623828 pLSG-IBA wt1 5µg
15633828 pLSG-IBA wt2 5µg
15643828 pLSG-IBA 3 5µg
15653828 pLSG-IBA 5 5µg
15663828 pLSG-IBA 23 5µg
15683828 pLSG-IBA 33 5µg
15693828 pLSG-IBA 35 5µg
15603838 pLSG-IBA 43 5µg
15613838 pLSG-IBA 45 5µg
15623838 pLSG-IBA 62 5µg
15633838 pLSG-IBA 63 5µg
15643838 pLSG-IBA 64 5µg
15653838 pLSG-IBA 65 5µg
15663838 pLSG-IBA 102 5µg
15673838 pLSG-IBA 103 5µg
15683838 pLSG-IBA 104 5µg
15693838 pLSG-IBA 105 5µg
15603848 pLSG-IBA 123 5µg
15613848 pLSG-IBA 142 5µg
15623848 pLSG-IBA 143 5µg
15633848 pLSG-IBA 144 5µg
15643848 pLSG-IBA 145 5µg
15653848 pLSG-IBA 162 5µg
15663848 pLSG-IBA 164 5µg
15673848 pLSG-IBA 167 5µg
15683848 pLSG-IBA 168 5µg
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 27 options available.
27 options
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Artikelnummer. 15673828 Leverandør IBA Lifesciences Leverandørnr. 54825001

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Beskrivelse

Transfer vectors that allow for high-level expression in insect cells by introducing the gene of interest into the Polyhedrin gene locus of AcMNPV DNA by homologous recombination.

pLSG-IBA Baculo virus (insect cells) expression vectors

The pLSG-IBAwt1 vector carries the Polyhedrin promoter for high-level expression in insect cells.

  • Co-transfection with BacPAK6 linearized AcMNPV DNA (Clontech) or circular flashBAC modified AcMNPV DNA (Oxford Expression Technologies) allows the generation of recombinant baculovirus at very high efficiency through reconstitution of an essential gene (ORF 1629) and elimination of wild type virus to great extent.
  • Cloning and expression of recombinant proteins in insect cells
  • Polyhedrin promoter drives high-level expression of the insert
  • Ampicillin resistance and ColE1 origin of replication (pUC) support propagation in E. coli

Please note that cloning into pLSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pLSG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.

Tekniske data

Format Liquid
Til brug med (udstyr) StarGate™ System, Insect Host Cells
Inducerende middel BacPAK6 linearized A cmNPV DNA (Clontech) or circular flashBAC modified A cmNPV DNA (Oxford Expression Technologies)
Inkluderer pUC ori, Ampicillin resistance
pH 8
Promotor Polyhedrin
Produkttype Acceptor Vector for bacula (insect cell) expression
Opbevaringsbuffer 20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg
Indhold og opbevaring 2°C to 8°C for frequent usage, -20°C for long-term storage
Protein tag GST Tag for N-terminal
Koncentration 250 ng/μL
Mængde 5 μg
Vektor pLSG-IBA 25
Farve Clear
Til brug med (applikation) Cloning Genes of Interest for Use in Functional Expression, Purification, Detection, Immobilization or Separation of Recombinant Proteins
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