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![Invitrogen™ Human RAF1 (cRAF) [Y340D, Y341D], GST Tag Recombinant Protein 1mg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/default.jpg-650.jpg)
Invitrogen™ Human RAF1 (cRAF) [Y340D, Y341D], GST Tag Recombinant Protein
Human RAF1 (cRAF) [Y340D, Y341D], GST Tag Recombinant Protein
Brand: Invitrogen™ PV4046
Additional Details : Weight : 0.00900kg
Description
RAF1 (cRAF) is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated RAF1 can phosphorylate and activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylates and activates the serine⁄threonine specific protein kinases ERK1 and ERK2. The RAF1 Y340D Y341D mutant is constitutively active.Specifications
| NP_002871, Y340D, Y341D | |
| Protein Assays and Analysis, Kinase Assay | |
| 66.4kDa | |
| 1mg | |
| For research use only. Not for use in diagnostic procedures | |
| RAF1 (cRAF) Y340D Y341D | |
| RAF1 (cRAF) Y340D Y341D was pre-diluted in enzyme dilution buffer and assayed in 25mM Tris (pH 7.5), 10mM MgCl2, 0.5mM EGTA, 0.5mM Na3VO4, 5mM β-glycerophosphate, 2.5mM DTT, 0.01% Triton™ X-100, 100μM ATP, 200μg/mL MAP2K1, inactive (P3093) and trace [32P]-γ-ATP for 10 minutes at 30°C | |
| Amino acids 306-648 | |
| 220nmoles of phosphate transferred to MAP2K1, inactive (P3093) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 0.25μg/mL | |
| GST Tag | |
| Frozen |
| 0.31mg/mL | |
| Frozen | |
| RAF1 (cRAF) [Y340D] [Y341D] Recombinant Human Protein | |
| –80°C | |
| 1 Each | |
| RAF1 (cRAF) [Y340D] [Y341D] Recombinant Human Protein | |
| RAF | |
| RAF1 (cRAF) [Y340D] [Y341D] Recombinant Human Protein | |
| Human | |
| Baculovirus | |
| 80% by SDS-PAGE |
For Research Use Only. Not for use in diagnostic procedures.