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Gibco™ pOptiVEC™-TOPO™ TA Cloning™ Kit
Express Kit is designed for efficient growth and transfection of dihydrofolate reductase- deficient (DHFR) Chinese hamster ovary (CHO) DG44 cells in suspension culture
Brand: Gibco™ 12744017
Additional Details : Weight : 0.00450kg
Description
- The OptiCHO™ Express Kit is designed for efficient growth and transfection of dihydrofolate reductase- deficient (DHFR) Chinese hamster ovary (CHO) DG44 cells in suspension culture.
- The kit provides a complete workflow protocol as well as the necessary components for efficient transfection of DHFR DG44 cells in a serum-free environment and subsequent stable cell line development, including:
- pOptiVEC™-TOPO™ vector - a bicistronic cloning vector containing a DHFR gene for stable cell line development in CHO-DG44 cells (Figure 1); the vector is TOPO™ adapted and enables cloning of a PCR product containing your gene of interest with >85% efficiency
- FreeStyle™ MAX Transfection Reagent - an animal origin-free transfection reagent offering high transfection efficiencies and consistent results
- CD OptiCHO™ Medium - a chemically defined, protein-free medium (without hypoxanthine or thymidine) specifically designed for high-density suspension cell culture of recombinant CHO-DG44 cells and secreted protein yield
- CD DG44 Medium - a chemically defined, protein-free medium supplemented with hypoxanthine and thymidine to support optimal growth of DG44 cells in suspension culture
- DG44 cells - preadapted to CD DG44 medium and selected for superior cell growth and transfection efficiencies
- In addition, the OptiCHO™ Express Kit provides GIBCO™ OptiPRO™ SFM for improved DNA-lipid complex formation, L-glutamine (provided separately for increased media stability), and Pluronic™ F-68 to control shear forces in suspension cultures.
- Storage: Store Pluronic™ F-68 at 15°C to 30°C. Store CD DG44 Medium, OptiPRO™ SFM, CD OptiCHO™ Medium, and FreeStyle™ MAX Transfection Reagent at 2°C to 8°C in the dark. Store L-glutamine, pOptiVEC™-TOPO™ vector, CMV Forward Sequencing Primer, and EMCV IRES Reverse Sequencing Primer at -5°C to -20°C. Store One Shot™ TOP10 Chemically Competent E. coli cells at -80°C. Store DG44 cells in liquid nitrogen.
Specifications
TOPO™-TA | |
Transfection | |
Cloning | |
Gibco™ | |
CMV | |
1 Kit | |
TOPO-TA Vectors |
Constitutive | |
Kit | |
Protein Production, Stable Cell Line Development | |
Cloning Kit | |
Untagged | |
HT (-) Media, MTX (Methotrexate) |
For Research Use Only. Not for use in diagnostic procedures.