Invitrogen™ Dynabeads™ M-280 Sheep Anti-Mouse IgG

Description

Dynabeads M-280 Sheep anti-Mouse IgG beads are 2.8-μm monosized, monodispersed, superparamagnetic beads with affinity-purified polyclonal sheep anti-mouse IgG covalently bound to the bead surface. These beads are intended for use as a solid support for simple and efficient binding of mouse IgGs and their target proteins. The uniform, monodisperse, and non-porous Dynabeads magnetic beads make them an ideal choice for applications such as binding immunoglobulins, protein purification, sandwich immunoassays, immunoprecipitation (IP), Co-immunoprecipitation (Co-IP), and isolation of cells and microorganisms. The polyclonal sheep antibody binds mouse IgG1, IgG2a, and IgG2b, and, with a lower reactivity, mouse IgG3 and IgM. Human cross-reactivity is minimal.

Benefits of Dynabeads M-280 Sheep anti-Mouse IgG beads include:

• Speed—isolate protein in less than 40 minutes
• Selectivity—binds most mouse IgG subclasses, very low non-specific binding
• Flexibility—no columns, centrifugations, or time-consuming pre-clearing required
• Automation ready—automation protocols available with the KingFisher Sample Purification instruments

Great signal to noise ratio

Dynabeads M-280 Sheep anti-Mouse IgG beads covalently couple the secondary sheep antibodies to a hydrophobic surface, which means the non-specific hydrophobic interactions are reduced to a minimum. Since this interaction is the main driver of unwanted protein binding, Dynabeads magnetic beads help ensure a great signal-to-noise ratio. This Dynabeads magnetic bead platform binds any mouse IgG antibody in a solution and can therefore become a powerful tool in affinity purification.

Dynabeads immunoprecipitation protocol is fast and easy

Add the target specific primary mouse IgG antibody directly to the sample (indirect technique) or first pre-coat the target antibody onto the Dynabeads M-280 Sheep Anti-Mouse IgG beads (direct technique). Regardless of which technique is used, when the Dynabeads M-280 Sheep anti-Mouse IgG beads are mixed with the sample, they bind to the target. The manual IP protocol is simple and can be performed in less than 40 minutes with 3 easy bind-incubate-elute steps. The procedure does not require any pre-clearing due to the low non-specific binding and uses very little antibody while still obtaining a high IP yield, thus saving both time and cost per IP.

The antibody-coated beads can be used in a variety of IP applications such as Co-IP, chromatin IP (ChIP/ChIP sequencing), RNA IP (RIP, RIP sequencing), small-scale IgG purification, and protein purification. The isolated pure target protein can be used in downstream western blot, mass spectrometry analysis, sequencing, etc.

Automation-ready for use with KingFisher purification systems

The 2.8-μm magnetic beads are ideal for high-throughput enrichment and can be automated using any of our KingFisher sample purification systems.

Commercial supply

Our manufacturing sites are ISO 13485-certified. Bead characteristics and manufacturing conditions help ensure consistency and batch reproducibility making them an ideal choice for commercial supply. If you are in the process of customizing Dynabeads Sheep anti-Mouse IgG beads in a commercial product or service or need a larger bulk volume of this product, please contact us at oemdynal@lifetech.com or see our Dynabeads OEM page.

Learn more about Dynabeads magnetic beads and KingFisher Sample Purification Systems:

• See our immunoprecipitation selection table, data, and references
• Dynabeads magnetic beads selection guide
• See magnets for Dynabeads magnetic beads separations
• KingFisher automation protocols
• Watch a video about the KingFisher Flex instrument

Order Info

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