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IBA Lifesciences pCSG-IBA Mammalian Expression Vector with No Secretion

Artikelnummer. 15663858 Shop alle IBA Lifesciences produkter
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Mængde:
5 μg
Pakningsstørrelse:
5µg
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Artikelnummer. 15663858

Brand: IBA Lifesciences 55035001

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Beskrivelse

Designed for high-level episomal expression in mammalian hosts under G418 selection. IBA Lifesciences GmbH Mammalian Expression Vector with No Secretion is a large expression vector with universal features for transient expression as well as for generation of stable cell lines (NeoR).

pCSG-IBA mammalian expression vectors

  • Designed for high-level episomal expression in mammalian hosts under G418 selection containing the following elements:
    • Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells
    • Episomal replication through Epstein Barr Virus replication origin (oriP) and nuclear antigen encoded by EBNA-1 in human, primate and canine cells and through SV40 replication origin in cells latently infected with SV40 or that express the SV40 large T antigen (e.g. HEK293T, COS-1, COS-7)
  • Stable cell lines can be selected by the neomycin resistance gene (NeoR).
  • In addition, the human cytomegalovirus (CMV) immediate-early promoter enables a high-level expression in a wide range of mammalian cells.

Please note that cloning into pCSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. Besides to the direct cloning of the gene of interest into pCSG-IBA vectors with Esp3I, another option via a so-called entry-vector is possible.

Tekniske data

Konstitutivt eller inducerbart system Constitutive
Markør Gene Ampicillin
Promotor CMV
Produkttype Vector
Udvælgelsesagent (eukaryot) Geneticin™ (G-418)
Indhold og opbevaring Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage
Protein tag His Tag (6x)
Mængde 5 μg
Vektor pCSG-IBA35
Til brug med (applikation) Episomal expression (oriP, EBNA-1)
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