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Beskrivelse
Isoamy alcohol is routinely used in molecular biology, especially in the purification of DNA. Isoamyl alcohol is also used in the extraction of ethidium bromide from DNA solutions and in the radiolabeling of RNA transcripts in nuclei that have been isolated from tissue.
- Widely used in conjunction with phenol and chloroform (25:24:1—phenol:chloroform:isoamyl alcohol) for the removal of proteins from the nucleic acid solutions by extraction
- Isoamyl alcohol is added to the phenol solution to help inhibit RNase activity and to help prevent the solubilization in the phenol phase of long RNA molecules with long poly(A) portions
- It will also help in reducing the foaming during the extraction process
Isoamy alcohol has various miscellaneous uses, including:
- HPLC analysis of various pharmaceuticals and metabolites
- Analysis of oxidized and reduced pyridine nucleotides and adenylates in organic phenol extracts from mitochondria
- Isolation of mRNA from a thermophilic cyanobacterium
- Solvent for fats, resins and alkaloids
- Manufacturing of isoamyl (amyl) compounds, isovaleric acid, mercury fulminate, pyroxylin, artificial silk, lacquers and smokeless powders
- For dehydrating celloidin solutions
- For determining fat in milk
Tekniske data
Tekniske data
| Smeltepunkt | -117.2°C (literature) |
| Tæthed | 0.81g/mL (water=1) at 25°C (Lit.) |
| Kogepunkt | 131°C (literature) |
| Brydningsindeks | n20/D 1.406 (Lit.) |
| Mængde | 500 mL |
| Opløselighedsinformation | Miscible with alcohol, ether, benzene, chloroform, petroleum ether, glacial acetic acid, oils. |
| Dampdensitet | 3.04 (air=1) (Lit.) |
| Formel vægt | 88.15 |
| Damptryk | 2.4mmHg at 20°C, 2.37mmHg at 25°C (Lit.) |
| Procent renhed | ≥99% |
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Produkttitel
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