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Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ MAX Efficiency™ DH5α-T1R E. coli
Designed for cloning PCR products directly from a PCR reaction in just 5 minutes
6080.00 DKK - 10870.00 DKK
Specifications
| Bacterial or Yeast Strain | DH5α |
|---|---|
| Cloning Method | TOPO™-TA |
| Content And Storage | Box 1: • Topoisomerase I-activated pCR™II-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid. Store at -68 to -85°C. |
| Format | Kit |
| Product Line | One Shot™ |
Includes: Topoisomerase I-activated pCR™II-TOPO™ vector; PCR buffer; Salt solution; dNTPs; Control template; T3, T7, M13 forward, and M13 reverse primers; Control PCR primers; Sterile water; One Shot™ Chemically Competent or Electrocomp™ E. coli; S.O.C. medium; Supercoiled pUC19 control plasmid
| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
|
10053172
|
Invitrogen™
K462040 |
50 Reactions |
10870.00 DKK
Each |
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|
10738414
|
Invitrogen™
K462001 |
25 Reactions |
6080.00 DKK
Each |
Please sign in to purchase this item. Need a web account? Register with us today! | |||||
Description
Uses a pCR™-TOPO™ Vector with covalently bound topoisomerase I for fast cloning and recombinants.
- 3'-T overhangs for direct ligation of Taq-amplified PCR products
- Choice of T7 (pCR2.1-TOPO) or T7 and SP6 (pCRII-TOPO) promoters for in vitro RNA transcription and sequencing. Each vector also contains M13 forward and reverse primer sites for sequencing.
- EcoR I sites flanking the PCR product insertion site for easy excision of inserts
- Kanamycin and ampicillin resistance genes for your choice of selection in E. coli
- Easy blue/white colony screening for selection of recombinants
Cloning, PCR Cloning
Specifications
| DH5α | |
| Box 1: • Topoisomerase I-activated pCR™II-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid. Store at -68 to -85°C. |
|
| One Shot™ | |
| T7, SP6 |
| TOPO™-TA | |
| Kit | |
| Cloning Kit | |
| TOPO-TA Cloning Vectors |
For Research Use Only. Not for use in diagnostic procedures.
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Product Title
Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ MAX Efficiency™ DH5α-T1R E. coli