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Invitrogen™ SuperScript™ One-Cycle cDNA Kit for use with Thermo Scientific™ One-Cycle Assays

Conveniently contains all of the reagents required for double-stranded cDNA synthesis within the GeneChip™ workflow

Brand: Invitrogen™ A10752030

Product Code. 10648524

21430.00 DKK / Each

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Includes: 50μM T7-Oligo(dT) Primer, 5X 1st Strand Reaction Mix, 0.1M DTT, 10mM dNTP, SuperScript™ II (200u/μL), 5X 2nd Strand Reaction Mix, E.Coli DNA Ligase, E.Coli DNA Polymerase I, RNase H, T4 DNA Polymerase, 0.5M EDTA, RNase-free Water

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Description

The kit includes Invitrogen's proprietary RNase H reduced activity SuperScript™ II Reverse Transcriptase and a qualified oligo (dt)-containing primer to perform reactions standard to Affymetrix– protocol. Designed to consistently produce higher yields of full-length cDNA for Affymetrix One-Cycle assays using either total RNA or poly A+ selected RNA (mRNA).

Order Info

Shipping Condition: Dry Ice

Specifications

Content And Storage	50 μM T7-Oligo(dT) Primer, 5X 1st Strand Reaction Mix, 0.1M DTT, 10 mM dNTP, SuperScript™ II (200u⁄ul), 5X 2nd Strand Reaction Mix, E.Coli DNA Ligase, E.Coli DNA Polymerase I, RNase H, T4 DNA Polymerase, 0.5M EDTA, RNase-free Water All components are guaranteed stable for 6 months when properly stored.
Format	Kit
Final Product Type	cDNA (Double-stranded)
Labeling Target	cDNA
Includes Label or Dye	No
Labeling Method	Direct Labeling
Product Type	cDNA Kit
Shipping Condition	Dry Ice

Detection Method	Biotin-based
Label or Dye	Biotin
Sample Type	Poly(A+) RNA, Total RNA
For Use With (Application)	For generating double-stranded cDNA
For Use With (Equipment)	Affymetrix GeneChip™
Product Line	SuperScript™
Quantity	30 Reactions

Product Suggestions Documents

Documents

Product Information

Manuals

SuperScript One-Cycle cDNA Kit Version: 5 Aug 2008 File size: 101 kB

Certificates

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Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number2599195	Certificate TypeCertificate of Analysis	Date01/05/2023	Catalog NumberA10752030
Lot Number1626951	Certificate TypeCertificate of Analysis	Date19/07/2017	Catalog NumberA10752030
Lot Number1362089	Certificate TypeCertificate of Analysis	Date15/04/2017	Catalog NumberA10752030
Lot Number1667544	Certificate TypeCertificate of Analysis	Date14/04/2017	Catalog NumberA10752030
Lot Number1207580	Certificate TypeCertificate of Analysis	Date13/04/2017	Catalog NumberA10752030

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Invitrogen™ SuperScript™ One-Cycle cDNA Kit for use with Thermo Scientific™ One-Cycle Assays > 30 reactions

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