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Invitrogen™ Platinum™ PCR SuperMix High Fidelity
Platinum™ PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification.
Brand: Invitrogen™ 12532024
Additional Details : Weight : 0.03000kg
Description
Platinum™ PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. You need only add template and primers, reducing set-up time by half (see figure).
With Platinum™ PCR SuperMix, High Fidelity, you will:
• Achieve greater than six-times higher fidelity than Taq DNA polymerase
• Amplify fragments up to 15 kb
• Produce products that are mixed blunt and 3-A' ends; however, the majority of ends will have a 3-A' overhangs
• Minimize PCR optimization
Using Platinum™ PCR SuperMix, High Fidelity
Platinum™ PCR SuperMix, High Fidelity, is for high-specificity, high-fidelity DNA amplification applications, such as cloning or mutagenesis. High fidelity is provided by a mixture of Taq DNA polymerase and the proofreading Pyrococcus species GB-D polymerase. High specificity is achieved by anti-Taq antibodies, allowing for automatic 'hot-start' PCR. This hot-start capability increases specificity and yield and allows for room-temperature reaction assembly.
Specifications
| Platinum PCR SuperMix High Fidelity (4 x 56.25 mL) Store at -20°C in a non-frost-free freezer. |
|
| Platinum Taq DNA Polymerase High Fidelity | |
| Wet or Dry Ice | |
| 1.1X | |
| Built-In Hot Start | |
| Mixed | |
| 15 kb or less |
| Low | |
| Standard | |
| Hot-start PCR, High-fidelity PCR | |
| 6X | |
| 5000 Reactions | |
| SuperMix or Master Mix |
For Research Use Only. Not for use in diagnostic procedures.