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Invitrogen™ Platinum™ PCR SuperMix High Fidelity
Platinum™ PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification.
4055.00 DKK - 121700.00 DKK
Specifications
GC-Rich PCR Performance | Low |
---|---|
Polymerase | Platinum Taq DNA Polymerase High Fidelity |
Reaction Speed | Standard |
Shipping Condition | Wet or Dry Ice |
For Use With (Application) | Hot-start PCR, High-fidelity PCR |
Includes: Platinum PCR SuperMix High Fidelity is supplied at 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 100 amplification reactions of 50μL each are provided (4 x 1.125mL). Platinum PCR SuperMix High Fidelity Components: 22U/mL complexed recombinant Taq DNA polymerase, Pyrococcus species GB-D thermostable polymerase, and Platinum Taq Antibody; 66mM Tris-SO4 (pH 8.9); 19.8mM (NH4)2SO4; 2.4mM MgSO4; 220μM dNTPs; and stabilizers.
Product Code | Brand | No. of Reactions | Price | Quantity & Availability | |||||
---|---|---|---|---|---|---|---|---|---|
Product Code | Brand | No. of Reactions | Price | Quantity & Availability | |||||
10545013
|
Invitrogen™
12532016 |
100 Reactions |
4055.00 DKK
100 reactions |
In Stock Log in to see stock. |
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10175622
|
Invitrogen™
12532024 |
5000 Reactions |
121700.00 DKK
5000 reactions |
Estimated Shipment: 12-07-2024 Log in to see stock. |
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Description
Platinum™ PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. You need only add template and primers, reducing set-up time by half (see figure).
With Platinum™ PCR SuperMix, High Fidelity, you will:
• Achieve greater than six-times higher fidelity than Taq DNA polymerase
• Amplify fragments up to 15 kb
• Produce products that are mixed blunt and 3-A' ends; however, the majority of ends will have a 3-A' overhangs
• Minimize PCR optimization
Using Platinum™ PCR SuperMix, High Fidelity
Platinum™ PCR SuperMix, High Fidelity, is for high-specificity, high-fidelity DNA amplification applications, such as cloning or mutagenesis. High fidelity is provided by a mixture of Taq DNA polymerase and the proofreading Pyrococcus species GB-D polymerase. High specificity is achieved by anti-Taq antibodies, allowing for automatic 'hot-start' PCR. This hot-start capability increases specificity and yield and allows for room-temperature reaction assembly.
Order Info
Approved for shipment on Wet or Dry Ice
Specifications
Low | |
Standard | |
Hot-start PCR, High-fidelity PCR | |
6X | |
Mixed | |
15 kb or less |
Platinum Taq DNA Polymerase High Fidelity | |
Wet or Dry Ice | |
1.1X | |
Built-In Hot Start | |
SuperMix or Master Mix |
For Research Use Only. Not for use in diagnostic procedures.