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Invitrogen™ BioPrime™ DNA Labeling System

Specifically designed for use in the preparation of biotinylated probes

Brand: Invitrogen™ 18094011

4607.50 DKK valid until 2025-12-31
Use promo code "24344" to get your promotional price.

Product Code. 10553853

5330.00 DKK / 30 reactions

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Includes: 700μL 2.5X Random Primers Solution; 175μL 10X dNTP Mixture; 100μg Control DNA; 35μL Klenow Fragment (40U/μL); 500μL Stop Buffer; 1.25mL Distilled Water

This item is not returnable. View return policy

Description

Random primers (octamers) are annealed to the denatured DNA template and extended by Klenow fragment in the presence of biotin-14-dCTP to produce sensitive biotinylated-DNA probes for use in the nonradioactive detection of DNA and RNA. With the BioPrime™ DNA Labeling System, considerable net DNA synthesis occurs resulting in a 10–40 fold amplification of the probe. Probes made with this product have been used for hybridization to Southern and northern blots, plaque and colony lifts, and for in situ hybridization to chromosome spreads.

Particularly well suited for situations in which the DNA is limited
Requires less template DNA than nick translation
Amplifies template to provide an increased amount of biotinylated probe
Labels 50 to 500ng of template DNA in one reaction

Array CGH, DNA and RNA Purification and Analysis, Gene Expression Analysis and Genotyping, Genotyping and Genomic Profiling, Northern Blotting, Nucleic Acid Gel Electrophoresis and Blotting, Southern Blotting

Order Info

Shipping Condition: Approved for shipment on Wet or Dry Ice

Specifications

Content And Storage	• 700 μl 2.5X Random Primers Solution • 175 μl 10X dNTP Mixture • 100 μg Control DNA • 35 μl Klenow Fragment (40 U/μl) • 500 μl Stop Buffer • 1.25 ml Distilled Water Store at -20°C in a non-frost-free freezer.
Label or Dye	Biotin
Labeling Target	DNA (General), Genomic DNA
Labeling Method	Direct Labeling
Product Type	DNA Labeling System
Shipping Condition	Approved for shipment on Wet or Dry Ice

Detection Method	Biotin-based
Final Product Type	Probes (Labeled DNA)
Includes Label or Dye	Yes
Product Line	BioPrime™
Quantity	30 Reactions

Product Suggestions Documents

Documents

Product Information

Manuals

BioPrime DNA Labeling System, 30 rxn Version: 7 February 2006 File size: 53.2 kB

Certificates

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Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number3048080	Certificate TypeCertificate of Analysis	Date21/01/2025	Catalog Number18094011
Lot Number3026229	Certificate TypeCertificate of Analysis	Date25/10/2024	Catalog Number18094011
Lot Number2649744	Certificate TypeCertificate of Analysis	Date26/02/2024	Catalog Number18094011
Lot Number2553520	Certificate TypeCertificate of Analysis	Date10/01/2023	Catalog Number18094011
Lot Number2500631	Certificate TypeCertificate of Analysis	Date21/07/2022	Catalog Number18094011

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