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  7. Applied Biosystems™ Power SYBR™ Green RNA-to-CT™ 1-Step Kit

Applied Biosystems™ Power SYBR™ Green RNA-to-CT™ 1-Step Kit

Product Code. 10229194 Shop All Applied Biosystems Products
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Product Code. No. of Reactions Unit Size
10229194 500 Reactions 200 reactions
10734227 100 Reactions Each
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Product Code. 10229194 Supplier Applied Biosystems™ Supplier No. 4389986
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Descripción

The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.

The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.

Get sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction. The Power SYBR™ Green RNA-to-CT™ 1-Step kit has been formulated to ensure maximum sensitivity and reliability. The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets. It includes:

  • AmpliTaq™ Gold DNA Polymerase UP for hot start qPCR, minimizing non-specific product formation
  • ArrayScript™ UP Reverse Transcriptase, an engineered RT that produces high yields of full-length cDNA
  • An additive that reduces primer dimer formation, reducing non-specific products that can cause false positive signals
  • RNase inhibitor, to prevent degradation of RNA templates
  • A proprietary ROX™ dye that serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations for superb precision on Applied Biosystems™ real-time PCR instruments.

Información de órdenes

Shipping Conditions: Wet Ice

TRUSTED_SUSTAINABILITY

Especificaciones

Content And Storage Sufficient for 500 reactions at 20 μL or 200 reactions at 50 μL reaction volume and includes:
• 1 x 5 mL tube of 2X master mix containing SYBR™Green 1 Dye, AmpliTaq Gold™ DNA Polymerase UP, dNTPs, Passive Reference 1,and optimized buffer components. Store at 2-8°C after opening.
• 1 x 80 μL tube of 125X RT enzyme mix containing ArrayScript™ UP Reverse Transcriptase, RNase Inhibitor. Store at -15 to -30°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Detection Method SYBR
PCR Method 1-step RT-qPCR
Polymerase AmpliTaq Gold DNA Polymerase
Reaction Speed Standard
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
Label or Dye SYBR Green
Passive Reference Dye ROX (Pre-mixed)
Product Line RNA-to-CT, SYBR
Product Type One-Step qRT-PCR Kit
Purity or Quality Grade UP (Ultra Pure)
Quantity 500 Reactions
Reverse Transcriptase ArrayScript™ UP
Run Time Standard
Shipping Condition Wet Ice
Sufficient For 500 Reactions at 20 μL
For Use With (Application) Gene Expression
No. of Reactions 500 Reactions
Sample Type RNA
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For Research Use Only. Not for use in diagnostic procedures.

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