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IBA Lifesciences Gravity flow Strep-Tactin™ 4Flow™ high capacity mini- column
Strep-Tactin™ 4Flow™ high capacity gravity flow mini- column to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins
Brand: IBA Lifesciences 2-1251-005
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Description
- Strep-Tactin™ 4Flow™ high capacity columns are designed for purifying Strep-tag™II and Twin-Strep-tag™ proteins via affinity chromatography in gravity flow
- Recommended for all protein classes due to the broad pH compatibility, excellent pressure stability, outstanding target purity (>95%), and high binding affinity
- 4% crosslinked agarose beads coupled to Strep-Tactin™ for straightforward purification of proteins with Strep-tag™II or a Twin-Strep-tag™
- Targeted elution: no tedious titration steps required and multiple regeneration cycles possible using 100 mM NaOH
- Ideal alternative for proteins that are difficult to elute from Strep-Tactin™XT 4Flow™ resins
- Biotinylated proteins also bind to Strep-Tactin™ 4Flow™ high capacity and need to be blocked using BioLock
Specifications
20 mg/mL resin | |
4% agarose, crosslinked | |
Resin Slurry | |
Tetrameric protein | |
Gravity Flow Column | |
Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin | |
Gravity flow column |
0.2 mL | |
50 to 150 ÎĽm | |
0.2 mL | |
Proteins tagged with Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin | |
Affinity chromatography equipment | |
Agarose | |
Pre-packed gravity flow column |
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Product Title
IBA Lifesciences Gravity flow Strep-Tactin™ 4Flow™ high capacity mini- column > Gravity flow 0.2 ml
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